Colletotrichum acutatum M11 can suppress the defence response in strawberry plants

Biological control of strawberry grey mold disease caused by Botrytis cinerea mediated by Colletotrichum acutatum extracts

“…Figure 6 shows that the genes FaPR1 (p = 0.1719), FaChi2-2 (p = 0.1227), FaCHI23 (p = 0.3431) and FaOGBG (p = 0.1829) that are regulated by SA (Graham et al 2003;van Loon et al 2006;Hael-Conrad et al 2018;Martinez-Zamora et al 2012) remained unchanged whereas genes associated to the ET signaling pathway FaETR1 (p = 0.0171), FaERS1 (p = 0.0427), and FaERF1 (p = 0.0321) (Hua and Meyerowitz 1998;Elias et al 2018;Merchante et al 2013) exhibited an up-regulation with respect to control plants. In the figure, we can observe that the genes associated to ROS scavenging FaCAT (p = 0.2993) (encoding catalase isozyme 1, Guerrero-Molina et al 2015) and FaPRX (p = 0.084) (encoding a class III plant peroxidase, Hael-Conrad et al 2018) did not change the level of expression confirming previous reports about the ability of C. acutatum M11 to suppress the oxidative burst (Tomas-Grau et al 2019). Evaluation of ROS accumulation confirmed that FEs did not bring about the accumulation of H 2 O 2 and O 2…”

“…Vascular bundles of central leaflets vein were observed under BF, blue light (U-MWB2) and UV light (329 nm). For the observation of collenchyma associated to vascular bundles, samples were prepared according to Tomas-Grau et al (2019) and incubated for 2 min in an aqueous solution of Astral Blue dye 0.05%. Samples were mounted on slides with 30% glycerol and examined with a fluorescence microscope (BX51 U-LH 100HG, Olympus, Germany).…”

“…Recently we have reported that a cell extract obtained from the local isolate M11 of the pathogenic fungus Colletotrichum acutatum caused the suppression of the oxidative burst making strawberry plants more sensitive to that pathogen (Tomas-Grau et al 2019). Taking into account that it has been reported that the oxidative burst sensitizes plants toward B. cinerea (Govrin and Levine 2000;Segal and Wilson 2017), we hypothesize that the fungal extract (FE) of the hemibiotroph C. acutatum M11 confers protection against the necrotrophic pathogen B. cinerea.…”

The fungal elicitor AsES requires a functional ethylene pathway to activate the innate immunity in strawberry

“…Production of H 2 O 2 , determined using a peroxidase‐dependant histochemical stain with 3,3´‐ diaminobenzidine (DAB) (Thordal‐Christensen et al 1997), was performed in foliar tissue as described by Tomas‐Grau et al (2019). Five plants were used per treatment (as described above), and three central leaflets were cut at 1, 2 or 3 dpp and immediately dipped into DAB 0.1% (p/v) solution in 10 m m MES buffer (pH 3.8).…”

Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina

“…Based on antecedents, we confirmed that M11 inhibits the plant's innate mechanism of defence and rapidly propagates throughout the plant, killing it as fast as 10dpi [65] . Moreover, M11 produces a diffusible compound that suppresses the biochemical, physiological, molecular and anatomical events associated with the defence response induced by an elicitor [66] . Unlike M11, isolate F7 induces a mechanism of defence in cv.…”