Collembase: a repository for springtail genomics and soil quality assessment

Timmermans, Martijn J. T. N.; Boer, M.E. de; Nota, Benjamin; Boer, Tjalf E. de; Mariën, Janine; Klein-Lankhorst, R. M.; Straalen, Nico M. van; Roelofs, Dick

doi:10.1186/1471-2164-8-341

Cited by 44 publications

(59 citation statements)

References 43 publications

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“…The F. candida strain used in our study may be infected by RLO as well. Bioinformatic analysis of a recently published EST dataset of the same strain (Timmermans et al 2007) revealed two sequences (GenBank: EV480534 and GE647879) which each harbored a fragment that showed high similarity to the published RLO sequence. Still, in our experiments with F. candida we can be reasonably sure that the effect of rifampicin and heat treatment can be attributed to Wolbachia, because these RLO seem to be restricted to the gut and the fatbody of F. candida (they are not associated with gonads (Czarnetzki and Tebbe 2004a)).…”

Section: Discussionmentioning

confidence: 98%

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Wolbachia endosymbiont is essential for egg hatching in a parthenogenetic arthropod

Timmermans

Ellers²

2008

Evol Ecol

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Wolbachia pipientis can induce a range of sex ratio distortions including parthenogenesis. Recently Wolbachia has been detected in the diploid, parthenogenetic, collembolan species Folsomia candida. In this paper we address the effect of Wolbachia on reproduction in F. candida. Wolbachia infection was removed by antibiotic and heat treatment, and quantitative PCR techniques confirmed the success of the treatments. Complete loss of Wolbachia-infection led to the production of normal clutch sizes, but was associated with full egg hatching failure. Our results demonstrate that F. candida is strictly dependent on Wolbachia to produce viable offspring. This is one of the few cases of obligatory Wolbachia infection in arthropods. Our data suggest a unique mechanism underlying Wolbachia-dependence of egg development. One of our more salient results is that hatching success increased in consecutive egg clutches of antibiotic-treated individuals, probably due to restoration of bacterial densities over time. These observations suggest that reproduction in F. candida is a threshold effect requiring a critical Wolbachia density as is hypothesized by the bacterial dosage model. Quantitative PCR analysis showed that heat or antibiotic treated individuals with egg hatching failure had low average bacterial densities, but bacterial densities were not significantly different from those of treated individuals with successfully eclosing eggs. Additional experiments with partially cured F. candida are needed to prove the dosage model.

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Section: Discussionmentioning

confidence: 98%

“…However, the recent establishment of an EST database for F. candida (Timmermans et al 2007) will greatly facilitate a more detailed molecular analysis of the genetic changes produced by Wolbachia infection.…”

Section: Discussionmentioning

confidence: 99%

Wolbachia endosymbiont is essential for egg hatching in a parthenogenetic arthropod

Timmermans

Ellers²

2008

Evol Ecol

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“…cDNA was synthesized from 1μg total RNA using the Omniscript Reverse Transcriptase Kit (Qiagen) and an Anchored Oligo(dT)20 primer (Invitrogen A/S, Naerum, Denmark) in a total volume of 13μl according to the manufacturer's instructions. Sequences for genes analysed were obtained from http://www.collembase.org (Timmermans et al, 2007). Primers were designed using Primer3 (Rozen and Skaletsky, 2000) and synthesized by MWG Eurofins (Ebersberg, Germany; Table1).…”

Section: Gene Expressionmentioning

confidence: 99%

Induced cold tolerance mechanisms depend on duration of acclimation in the chill sensitiveFolsomia candida(Collembola)

Waagner

Holmstrup

Bayley

et al. 2013

Journal of Experimental Biology

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SUMMARYDuring cold periods ectotherms may improve low temperature tolerance via rapid cold hardening (RCH) over a period of hours and/or long-term cold acclimation (LTCA) during days, weeks or months. However, the effect of duration and the major underlying mechanisms of these processes are still not fully understood. In the present study, the molecular and biochemical responses to RCH (1-3h) and LTCA (1-3days) and the corresponding benefits to survival were investigated using the chill-sensitive collembolan Folsomia candida. We investigated osmolyte accumulation, membrane restructuring and transcription of candidate genes as well as survival benefits in response to RCH and LTCA. RCH induced significant upregulation of targeted genes encoding enzymes related to carbohydrate metabolic pathways and genes encoding small and constitutively expressed heat shock proteins (Hsps), indicating that the animals rely on protein protection from a subset of Hsps during RCH and probably also LTCA. The upregulation of genes involved in carbohydrate metabolic processes initiated during RCH was likely responsible for a transient accumulation of myoinositol during LTCA, which may support the protection of protein and membrane function and structure. Membrane restructuring, composed especially of a significantly increased ratio of unsaturated to saturated phospholipid fatty acids seems to be a mechanism supplementary to activation of Hsps and myoinositol accumulation in LTCA. Thus, the moderate increase in cold shock tolerance conferred by RCH seems to be dominated by effects of Hsps, whereas the substantially better cold tolerance achieved after LTCA is dominated by post-transcriptional processes increasing membrane fluidity and cryoprotectant concentration.

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“…The first strand cDNA was synthesized using reverse transcriptase MMLV (Promega) and oligod(T) 15 according to the manufacturer's instructions. The b-actin gene was chosen as an internal control for hsp70 gene quantification (Timmermans et al 2007). The two genes were quantified by SYBR Green I assays using the primer set ACT1-F (GCGACGTTGAT ATCCGAAAG) and ACT1-R (AGGCAGTGATTTCCTT TTGC) for b-actin gene, and the primer set H7-1F (GG ACTTTGACAACCGAATGG) and H7-1R (CCACGGA AAAGATCAGCATT) for hsp70 gene.…”

Section: Transcriptional Responses Of Hsp70 In F Candidamentioning

confidence: 99%

Toxicity of profenofos to the springtail, Folsomia candida, and ammonia-oxidizers in two agricultural soils

Liu

Zheng

2012

Ecotoxicology

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Extensive use of organophosphorus insecticide profenofos (PFF) for agricultural and house-hold purposes has led to serious environmental pollution, with potential risk to organisms in the ecosystem. This study examined the toxicity of PFF to the soil springtail Folsomia candida and ammonia-oxidizers through a series of toxicity tests conducted on two agricultural soils. It was found that the survival, reproduction, hsp70 gene expression of F. candida and the soil potential nitrification rate (PNR) were sensitive to the PFF, whereas no apparent change was observed in the abundance of ammonia-oxidizers. The reproduction of F. candida was the most sensitive endpoint (mean 0.10 mg/kg of EC 50 value) for PFF, although the test was more time-consuming. The results of the acute toxicity tests suggested that the survival of F. candida could be considered as the most suitable bioindicator for fast screening of PFF toxicity because of its fast and easy test procedure. In addition, the hsp70 gene expression in F. candida and the PNR could be used as important parameters for assessment of PFF toxicity. The threshold concentration based on the obtained endpoints differed in the two soils, and consequently the soil property should be considered in toxicity assessments of contaminated soils.

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Collembase: a repository for springtail genomics and soil quality assessment

Cited by 44 publications

References 43 publications

Wolbachia endosymbiont is essential for egg hatching in a parthenogenetic arthropod

Wolbachia endosymbiont is essential for egg hatching in a parthenogenetic arthropod

Induced cold tolerance mechanisms depend on duration of acclimation in the chill sensitiveFolsomia candida(Collembola)

Toxicity of profenofos to the springtail, Folsomia candida, and ammonia-oxidizers in two agricultural soils

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