Collective Migrations of <i>Drosophila</i> Embryonic Trunk and Caudal Mesoderm-Derived Muscle Precursor Cells

Sun, Jui-Hung; Macabenta, Frank; Ákos, Zsuzsa; Stathopoulos, Angelike

doi:10.1534/genetics.120.303258

Cited by 12 publications

(13 citation statements)

References 188 publications

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“…These include mesoderm tube collapse, cell protrusions, suppression of adherens junction number, and promotion of mesoderm cell division (J. Sun, Macabenta, et al, 2020). Fog elicits cell‐shape change during gastrulation through the G‐protein‐coupled receptors Mist and Smog.…”

Section: Selected Topics In Genetics Development Regeneration and Dis...mentioning

confidence: 99%

New developments in the biology of fibroblast growth factors

Ornitz

Itoh

2022

WIREs Mechanisms of Disease

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The fibroblast growth factor (FGF) family is composed of 18 secreted signaling proteins consisting of canonical FGFs and endocrine FGFs that activate four receptor tyrosine kinases (FGFRs 1-4) and four intracellular proteins (intracellular FGFs or iFGFs) that primarily function to regulate the activity of voltagegated sodium channels and other molecules. The canonical FGFs, endocrine FGFs, and iFGFs have been reviewed extensively by us and others. In this review, we briefly summarize past reviews and then focus on new developments in the FGF field since our last review in 2015. Some of the highlights in the past 6 years include the use of optogenetic tools, viral vectors, and inducible transgenes to experimentally modulate FGF signaling, the clinical use of small molecule FGFR inhibitors, an expanded understanding of endocrine FGF signaling, functions for FGF signaling in stem cell pluripotency and differentiation, roles for FGF signaling in tissue homeostasis and regeneration, a continuing elaboration of mechanisms of FGF signaling in development, and an expanding appreciation of roles for FGF signaling in neuropsychiatric diseases.

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Section: Selected Topics In Genetics Development Regeneration and Dis...mentioning

confidence: 99%

New developments in the biology of fibroblast growth factors

Ornitz

Itoh

2022

WIREs Mechanisms of Disease

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“…Many developmental processes are very fast, e.g. mesoderm spreading, which takes only about 1 h to complete (Sun et al, 2020). As live imaging is ideal for such quick, dynamic processes, the ability to rapidly increase fluorescent signal while labelling specific cell groups is crucial.…”

Section: Results and Discussion Nanutrap Allows Early Labelling Of Nuclei In Vivomentioning

confidence: 99%

“…For example, mesoderm spreading in Drosophila takes only about 1 h (see Fig. 2A; Clark et al, 2011;Leptin, 2005;Sun et al, 2020) and even with a strong mesodermspecific zygotic driver, such as the twist (twi) enhancer, GFP expression is not detectable early enough to illuminate the process from its beginning (Clark et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

NaNuTrap: a technique for in vivo cell nucleus labelling using nanobodies

2021

Self Cite

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In vivo cell labelling is challenging in fast developmental processes because many cell types differentiate more quickly than the maturation time of fluorescent proteins making visualization of these tissues impossible with standard techniques. Here we present a nanobody-based method, Nanobody Nuclear Trap (NaNuTrap), which works with the existing Gal4/UAS system in Drosophila and allows for early in vivo cell nuclei labelling independent of the fluorescent protein's maturation time. This restores the utility of fluorescent proteins that have longer maturation times, such as those used in two-photon imaging, for live imaging of fast or very early developmental processes. We also present a more general application of this system, whereby NaNuTrap can convert cytoplasmic GFP expressed in any existing transgenic fly line into a nuclear label. This nuclear re-localization of the fluorescent signal can improve the utility of the GFP label, for example in cell counting, as well as resulting in a general increase in intensity of the live fluorescent signal. We demonstrate these capabilities of NaNuTrap by effectively tracking subsets of cells during the fast movements associated with gastrulation.

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“…Through in vivo imaging, we observed that fast-proliferating adult fat body precursors migrate from the thorax into the abdomen, accumulate at the abdominal ventral midline and spread laterally and dorsally on the inner surface of the abdominal epidermis. The migration of these precursors is, therefore, strikingly reminiscent of mesoderm migration during gastrulation in the embryo (Leptin and Grunewald, 1990; McMahon et al, 2008; Sun et al, 2020). During gastrulation, the cells that give rise to the mesoderm invaginate at the ventral midline, undergo epithelial-to-mesenchymal transition and migrate dorsally along the ectoderm.…”

Section: Discussionmentioning

confidence: 99%

“…In the embryo, FGF signaling mutants fail to spread their mesoderm from the ventral midline. Different roles have been attributed to FGF to explain this defect, such as promoting epithelial-to-mesenchymal transition, regulating proliferation and guiding migration as a chemoattractant cue (Muha and Müller, 2013; Sun et al, 2020). According to our results, FGF may not affect precursor proliferation or differentiation during adult adipogenesis.…”

Section: Discussionmentioning

confidence: 99%

FGF signaling promotes precursor spreading for adult adipogenesis in Drosophila

Lei

Huang

Yang

et al. 2022

Preprint

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Knowledge of adipogenetic mechanisms is essential to understand and treat conditions affecting organismal metabolism and adipose tissue health. In Drosophila, mature adipose tissue (fat body) exists in larvae and adults. In contrast to the well-known development of the larval fat body from the embryonic mesoderm, adult adipogenesis has remained mysterious. Furthermore, conclusive proof of its physiological significance is lacking. Here, we show that the adult fat body originates from a pool of undifferentiated mesodermal precursors that migrate from the thorax into the abdomen during metamorphosis. Through in vivo imaging, we found that these precursors spread from the ventral midline and cover the inner surface of the abdomen in a process strikingly reminiscent of embryonic mesoderm migration, requiring FGF signaling as well. FGF signaling guides migration dorsally and regulates adhesion to the substrate. After spreading is complete, precursor differentiation involves fat accumulation and cell fusion that produces mature binucleate and tetranucleate adipocytes. Finally, we show that flies where adult adipogenesis is impaired by knock down of FGF receptor Heartless or transcription factor Serpent display ectopic fat accumulation in oenocytes and decreased resistance to starvation. Our results reveal that adult adipogenesis occurs de novo during metamorphosis and demonstrate its crucial physiological role.

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Collective Migrations of Drosophila Embryonic Trunk and Caudal Mesoderm-Derived Muscle Precursor Cells

Cited by 12 publications

References 188 publications

New developments in the biology of fibroblast growth factors

New developments in the biology of fibroblast growth factors

NaNuTrap: a technique for in vivo cell nucleus labelling using nanobodies

FGF signaling promotes precursor spreading for adult adipogenesis in Drosophila

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