Collective diffusion coefficient of proteins with hydrodynamic, electrostatic, and adhesive interactions

Abstract

A theory is presented for C , the coefficient of the first-order correction in the density of the collective diffusion coefficient, for protein spheres interacting by electrostatic and adhesive forces. An extensive numerical analysis of the Stokesian hydrodynamics of two moving spheres is given so as to gauge the precise impact of lubrication forces. An effective stickiness is introduced and a simple formula for C in terms of this variable is put forward. A precise though more elaborate approximation for C is also developed. These and numerically exact expressions for C are compared with experimental data on lysozyme at pH 4.5 and a range of ionic strengths between 0.05M and 2M.

Colloidal interactions between monoclonal antibodies in aqueous solutions

“…For small volume fractions, one can relate the collective diffusion constant to the solute properties as [37][38][39] …”

Short-time diffusion of charge-stabilized colloidal particles: generic features

“…The enlargement of the latter is overcompensated by the former, at least at smaller ' (van den Broeck et al, 1981). Thus, in dispersions of moderately charged particles, such as bovine serum albumin or lysozyme proteins with sufficiently strong short-range attraction, the collective diffusion coefficient, d c ¼ d 0 K=Sð0Þ, can attain values smaller than d 0 (Cichocki & Felderhof, 1990;Bowen & Mongruel, 1998;Bowen et al, 2000;Prinsen & Odijk, 2007).…”

“…Dispersions of charged colloidal particles undergoing correlated Brownian motion form a particularly important class of soft-matter systems ubiquitously encountered in the chemical industry, food science and biology (Pusey, 1991;Bowen & Mongruel, 1998;Retailleau et al, 1999;Bowen et al, 2000;Riese et al, 2000;Koenderink et al, 2003;Gapinski et al, 2005;Prinsen & Odijk, 2007). The calculation of diffusion transport properties for these systems is challenging since one needs to cope, in addition to direct electrosteric and van der Waals interparticle forces, with the solvent-mediated hydrodynamic interactions (HIs).…”

Light Scattering and SAXS Study of AOT Microemulsion at Low Size Droplet

“…In this work, the microemulsion system is located in the L 2 region at studied temperatures and compositions investigated. Most works on collective diffusion coefficient of droplets and interactions of droplets have been done mainly on charged macromolecules, [3][4][5][6][7][8][9][10][11][12][13][14][15], for example bimolecular, [3][4][5][6][7][8][9][10], as DNA, proteins and amino acids, micelles, [11], and polymer latexes, [15], in water (as polar solvent) at low ionic strength. The strong, long range electrostatic interactions between the particles or droplets in these systems lead to remarkable structure effects.…”