Collective curvature sensing and fluidity in three-dimensional multicellular systems

Tang, Wenhui; Das, Amit; Pegoraro, Adrian F.; Han, Yu; Huang, Jessie; Roberts, David A.; Yang, Haiqian; Fredberg, Jeffrey J.; Kotton, Darrell N.; Bi, Dapeng; Guo, Ming

doi:10.1038/s41567-022-01747-0

Cited by 20 publications

(17 citation statements)

References 48 publications

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“…14 Due to the difficulty in controlling the pore at a cellular scale with current fabrication methods, mechanobiology has been studied mostly with computer-in silico models 15 or evaluated in single blocks and curved substrates, 16,17 using wavy landscapes, 18 microcurved channels, 19 or semispherical wells with different radii of curvature. 13,20 There are 524 articles on lens.org that relate scaffolds and mechanobiology in the past five years, and only 138 also include the term porous. There are 1,057 that relate scaffold and curvature, but only 225 include porous.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Due to the difficulty in controlling the pore at a cellular scale with current fabrication methods, mechanobiology has been studied mostly with computer- in silico models or evaluated in single blocks and curved substrates, , using wavy landscapes, microcurved channels, or semispherical wells with different radii of curvature. , …”

Section: Introductionmentioning

confidence: 99%

“…A single pore, in terms of pore size, geometry, curvature, and wall roughness, can influence different cell processes studied within the field of mechanobiology and curvotaxis. 10 These concepts are starting to gain attention since they explain certain phenomena that can affect the complete scaffold, like a failure in cell attachment due to high curved struts 11 or due to high shear stress at the wall of pore size, 12 different rates of migration in curved surfaces, 13 and differentiation due to mechanical cues. 14 Due to the difficulty in controlling the pore at a cellular scale with current fabrication methods, mechanobiology has been studied mostly with computer-in silico models 15 or evaluated in single blocks and curved substrates, 16,17 using wavy landscapes, 18 microcurved channels, 19 or semispherical wells with different radii of curvature.…”

Section: Introductionmentioning

confidence: 99%

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Review on Porous Scaffolds Generation Process: A Tissue Engineering Approach

Flores-Jiménez

García-González

Fuentes-Aguilar

2023

ACS Appl. Bio Mater.

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Porous scaffolds have been widely explored for tissue regeneration and engineering in vitro three-dimensional models. In this review, a comprehensive literature analysis is conducted to identify the steps involved in their generation. The advantages and disadvantages of the available techniques are discussed, highlighting the importance of considering pore geometrical parameters such as curvature and size, and summarizing the requirements to generate the porous scaffold according to the desired application. This paper considers the available design tools, mathematical models, materials, fabrication techniques, cell seeding methodologies, assessment methods, and the status of pore scaffolds in clinical applications. This review compiles the relevant research in the field in the past years. The trends, challenges, and future research directions are discussed in the search for the generation of a porous scaffold with improved mechanical and biological properties that can be reproducible, viable for long-term studies, and closer to being used in the clinical field.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Review on Porous Scaffolds Generation Process: A Tissue Engineering Approach

Flores-Jiménez

García-González

Fuentes-Aguilar

2023

ACS Appl. Bio Mater.

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“…Our findings and the generic minimal model of single-cell curvotaxis could also provide insights into the orientation and migration of cells in aggregates such as confluent cell monolayers on curved surfaces [44][45][46][47] . The competitions among cell-cell junction, cortical actin bending, and cell contractility could result in even richer emergent collective behaviors 44 .…”

Section: Implications To Curvotaxis On More Complex Curved Surfacesmentioning

confidence: 70%

Biphasic curvature-dependence of cell migration inside microcylinders: persistent randomness versus directionality

Wang

et al. 2022

Preprint

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Cell-scale curvature plays important roles in controlling cell and tissue behaviors. However, these roles have not been well quantified, and the underlying mechanisms remain elusive. We combine experiments with theory to study systematically the curvature-dependence of cell migration inside PDMS microcylinders. We find that persistence is positively correlated with speed, following the universal speed-persistence coupling relation, i.e., faster cells turn less. Cell migration inside microcylinders is anisotropic and depends on curvature in a biphasic manner. At small curvatures, as curvature increases, the average speed and anisotropy both increase, but surprisingly, the average persistence decreases. Whereas as the curvature increases over some threshold, cells detach from the surface, the average speed and anisotropy both decrease sharply but the average persistence increases. Moreover, interestingly, cells are found to leave paxillins along their trajectories (on curved but not planar surfaces), facilitating the assembly of focal adhesions of following cells. We propose a minimal model for the biphasic curvotaxis based on three mechanisms: the persistent random noise, the bending penalty of stress fibers, and the cell-surface adhesion. The findings provide a novel and general perspective on directed cell migration in the widely existing curved microenvironment of cells in vivo.

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Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales

et al. 2023

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Surface curvature both emerges from, and influences the behavior of, living objects at length scales ranging from cell membranes to single cells to tissues and organs. The relevance of surface curvature in biology has been supported by numerous recent experimental and theoretical investigations in recent years. In this review, we first give a brief introduction to the key ideas of surface curvature in the context of biological systems and discuss the challenges that arise when measuring surface curvature. Giving an overview of the emergence of curvature in biological systems, its significance at different length scales becomes apparent. On the other hand, summarizing current findings also shows that both single cells and entire cell sheets, tissues or organisms respond to curvature by modulating their shape and their migration behavior. Finally, we address the interplay between the distribution of morphogens or micro-organisms and the emergence of curvature across length scales with examples demonstrating these key mechanistic principles of morphogenesis. Overall, this review highlights that curved interfaces are not merely a passive by-product of the chemical, biological and mechanical processes but that curvature acts also as a signal that co-determines these processes.

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Collective curvature sensing and fluidity in three-dimensional multicellular systems

Cited by 20 publications

References 48 publications

Review on Porous Scaffolds Generation Process: A Tissue Engineering Approach

Review on Porous Scaffolds Generation Process: A Tissue Engineering Approach

Biphasic curvature-dependence of cell migration inside microcylinders: persistent randomness versus directionality

Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales

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