Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials

Abstract

Strategies to replace retinal photoreceptors lost to damage or disease rely upon the migration of replacement cells transplanted into sub-retinal spaces. A significant obstacle to the advancement of cell transplantation for retinal repair is the limited migration of transplanted cells into host retina. In this work, we examine the adhesion and displacement responses of retinal progenitor cells on extracellular matrix substrates found in retina as well as widely used in the design and preparation of transplantable scaffolds. The data illustrate that retinal progenitor cells exhibit unique adhesive and displacement dynamics in response to poly-l-lysine, fibronectin, laminin, hyaluronic acid, and Matrigel. These findings suggest that transplantable biomaterials can be designed to improve cell integration by incorporating extracellular matrix substrates that affect the migratory behaviors of replacement cells.

Neuronal substrates alter the migratory responses of nonmyelinating Schwann cells to controlled brain‐derived neurotrophic factor gradients

“…Regenerative strategies rely strongly upon the local migration of replacement and/or supplemental cells (Mishra, Pena, Redenti, & Vazquez, ; Thakur et al, ), which requires detailed knowledge of cell migratory capacity and response(s). Although cell replacement therapies using terminal SCs remain elusive (Couve, Lovera, Suzuki, & Schmachtenberg, ), SC migration has been reported to aid axonal regeneration and synaptic SC–MN connections during transplantation of SCs, individually and in combination with different stem‐like cells and biomaterials (Ban et al, ; Hyung et al, ; Jones et al, ; Xia et al, ; Xie et al, ; Yang et al, ; Zeng et al, ; Zhang et al, ).…”

“…Average nmSC morphology was represented using the cell shape index (CSI), a dimensionless parameter commonly used to denote the relative roundness of a cell: where A and P represent the surface area and perimeter of an individual cell, respectively. Average CSI values of 1 correspond to a perfectly round cell, whereas average values approaching 0 denote a purely elongated cell (Pena et al, ; Thakur et al, ). At least 25 cells ( n = 25 per testing condition) were analyzed to determine the average CSI values reported.…”

Performance of a glucose-reactive enzyme-based biofuel cell system for biomedical applications

“…Migration of HDFs with EBFC. HDF migration, that is, the movement behaviors of cells in a specific direction, is one of the critical characteristics for measuring the cellular motility, which is helpful for tissue or organ wound healing after device implantation 60 . Therefore, a cell migration study was performed by using a wound scratch assay, which is a well-known method to mimic an in vivo environment where an electrical device is implanted in the human body 61 .…”

Development of High-Resolution Three-Dimensional-Printed Extracellular Matrix Scaffolds and Their Compatibility with Pluripotent Stem Cells and Early Retinal Cells

“…In fact, one recent study that examined retinal progenitor cell migration and displacement on various ECM substrates suggests that incorporating these molecules into scaffolds could improve photoreceptor cell integration. 15 Further, one group claims to have shown evidence that matching the culture substrate to composition of the interphotoreceptor matrix (IPM) enhances pluripotent stem cell differentiation and maturation toward photoreceptors, 16 but this work has not yet been published in a peer-reviewed journal (only a conference abstract is available). Although naturally occurring polymers such as chitosan, 17 hyaluronic acid (HA) and methylcellulose, 18,19 and even decellularized retinal ECM 20,21 have been used for retinal tissue engineering applications, the fabrication approaches used in these studies did not allow precise control of the scaffold structure at scales that are relevant to photoreceptor cell organization and alignment.…”