Collection of Pancreatic Juice from Rats and Consequences of its Continued Loss

Colwell, Arthur R.

doi:10.1152/ajplegacy.1951.164.3.812

Cited by 31 publications

(13 citation statements)

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“…It may fill a gap in the armamentarium available for pancreas research. Thus, the values for juice and bicar bonate secretion, as well as bicarbonate con centration, correspond with those observed in partially restrained rats with ligated bile duct and continuous drainage of pancreatic secretion [19], This latter procedure, in com mon with the original method [6], induced jaundice. The original method [6] also gave rise to other metabolic disturbances.…”

Section: Discussionsupporting

confidence: 73%

“…Diversion of bile by ductal reimplanta tion into the duodenum is possible over a period of several months. It may be doubted whether the same is possible with implanta tion of artificial tubings, which are prone to obstruction already at a rather early postop erative stage [6,14,16]. Moreover, our expe rience shows that enzyme replacement ther apy is not necessary.…”

Section: Discussionmentioning

confidence: 73%

“…Consequently, proce dures that stop the flow of pancreatic juice also induce jaundice and liver damage that subsequently kills the animal [6,14,19], The problem of separation of the bile flow from pancreatic juice flow has been partly over come by the implantation of artificial cannu las for the diversion of bile [6,14,16].…”

Section: Introductionmentioning

confidence: 99%

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A Method for Inducing Exocrine Atrophy and Collecting Juice from the Nonatrophied Pancreas in the Rat

1985

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A method for the long-term diversion of bile in the rat is described. It was used to induce complete and uniform pancreatic exocrine atrophy, as confirmed by a marked loss of weight of the gland, very low pancreatic tissue amylase, and by histological examination. The technique induces changes in endocrinal function of the pancreas, since it increases pancreas insulin by a factor of 2.7, decreases glucagon by a factor of 0.19, and somatostatin by a factor of 0.6. The method was also used for collecting juice from the nonatrophied pancreas in the conscious rat, and may facilitate studies on pancreas physiology and pathophysiology.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 73%

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A Method for Inducing Exocrine Atrophy and Collecting Juice from the Nonatrophied Pancreas in the Rat

1985

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“…Among the most evident, the follow ing may be cited. Pancreatic juice usually is collected by the method of C o lw ell [5], Most authors ligate the proximal part of the bile duct, thereby producing an obstructive jaundice. Some authors, however, prevent this * With the support of the 'Nationaal Fonds voor Wetenschappelijk Geneeskundig Onderzoek' of Belgium.…”

mentioning

confidence: 99%

Exogenous and Endogenous Secretin Stimulation in the Conscious Rat

A¹,

Waele²,

Wissoco³

et al. 1974

Digestion

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The actions of intravenous secretin injection and of intraduodenal acid infusion were studied in conscious rats who had recovered from the operative procedure and were maintained at a normal body temperature. The basal secretion calculated per unit weight of pancreas was higher in the rat than in the dog, even when the basal endogenous secretin stimulation induced by gastric acid was suppressed by ligation of the pylorus. Intravenous injection of GIH secretin induced a dose-related increase in volume and bicarbonate outputs. A brief washing out of proteins occurred initially. The maximal secretory response to endogenous stimulation was less than after intravenous secretin injection. The rate of secretin release was the limiting factor of the endogenously stimulated secretion. Calculated per unit weight of gland, the maximal secretory response of the pancreas to secretin is smaller in the rat than in the dog.

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“…The initial slow rate of secretion in anaesthetized rats may be due to two factors. Firstly, it seems likely that anaesthesia and the immediate effects of operation may depress pancreatic secretion [Colwell, 1951;Scott, Graham and McCartney, 1940;Zucker, Newburger and Berg, 1932]. Secondly, in order to collect pure pancreatic juice and estimate flow rate, most workers insert a cannula into the most distal portion of the common bile duct, then either ligate the bile duct at the hilus of the liver [Dockray, 1972], drain bile to the exterior [Debray, de la Tour, Vaille, Roze and Souchard, 1962] or redivert bile to the duodenum [Love, 1957].…”

mentioning

confidence: 99%

Basal and Postprandial Pancreatic Secretion in Rats

Shaw¹,

Heath²

1973

Exp Physiol

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When pancreatic juice which was collected separate from bile was diverted from the duodenum of rats for 24 hr, the basal flow rate of juice and its output of protein increased greatly over values recorded immediately after operation. Similar increases were recorded when pancreatic juice was recirculated back into the duodenum for 24 hr. Infusion of bicarbonate or enzyme into the duodenum failed to affect these increases in secretion. Thus the hypersecretion of juice did not appear to be the result of loss of juice from the duodenum. Attempts were then made to estimate rates of pancreatic secretion without diverting bile from the common bile duct. Immediately after operation, rates of pancreatic secretion estimated indirectly from the differences between combined bile-pancreatic juice and bile alone were higher than in those rats in which pancreatic juice was collected by diverting bile from the common bile duct. This suggested that bile in some way assisted the passage of viscous pancreatic juice down the common bile duct. Indirect estimates of pancreatic secretion made 24 hr after operation were greater than the estimates made immediately after operation but were similar to those values obtained after 24 hr in rats from which pure pancreatic juice was collected. This was taken as evidence to support the view that the trauma associated with anaesthesia and surgery may depress the rate of pancreatic secretion and that hypersecretion of juice may indeed be a normal phenomenon. In contrast to the situation reported to exist in dogs, indirect estimates of the effects of feeding on pancreatic secretion were similar irrespective ofwhether secretions were diverted from or returned to the duodenum.

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Collection of Pancreatic Juice from Rats and Consequences of its Continued Loss

Cited by 31 publications

References 0 publications

A Method for Inducing Exocrine Atrophy and Collecting Juice from the Nonatrophied Pancreas in the Rat

A Method for Inducing Exocrine Atrophy and Collecting Juice from the Nonatrophied Pancreas in the Rat

Exogenous and Endogenous Secretin Stimulation in the Conscious Rat

Basal and Postprandial Pancreatic Secretion in Rats

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