Collection of highly germinative pseudochain conidia of Oidium neolycopersici from conidiophores by electrostatic attraction

Nonomura, Teruo; Matsuda, Yoshinori; Xu, Lingfei; Kakutani, Koji; Takikawa, Yuichi; Toyoda, Hideyoshi

doi:10.1016/j.mycres.2008.11.012

Cited by 37 publications

(51 citation statements)

References 24 publications

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“…Excised leaves were kept in a Petri dish to ensure windless conditions. These conditions were essential for the pathogens to produce conidial pseudochains of a maximum length (Oichi et al 2006;Nonomura et al 2009). In fact, pseudochain conidia of the present isolates fell down from the conidiophores when they were exposed to a wind stronger than 0.4 m/s.…”

Section: Resultsmentioning

confidence: 99%

“…Mature conidia were collected from pseudochains of conidiophores under digital microscopy using an electrostatic spore collection probe (Nonomura et al 2009) and transferred to healthy leaves of 14-day-old tomato seedlings that had been placed in an electrostatic screen chamber (ES-chamber) (an apparatus for preventing airborne pathogens from entering the chamber) to prepare pathogen-free seedlings. After repeating this inoculation procedure three times, the obtained cultures were maintained as a new isolate (KTP-05).…”

Section: Methodsmentioning

confidence: 99%

“…We targeted conidiophores for microscopic observations, because of the easy and rapid detection of conidial pseudochains on them (Oichi et al 2004(Oichi et al , 2006. Pseudochain formation is common in the tomato powdery mildew pathogens (Jones et al 2001;Kiss et al 2001), and the number of conidia in the pseudochain is inherited (Oichi et al 2006;Nonomura et al 2009). …”

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confidence: 99%

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Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Nonomura¹,

Matsuda²,

Yamashita³

et al. 2013

Plant Prot. Sci.

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Nonomura T., Matsuda Y., Yamashita S., Akahoshi H., Takikawa Y., Kakutani K., Toyoda H. (2013): Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes. Plant Protect. Sci., 49 (Special Issue): S33-S40.In our routine surveys for the powdery mildew disease in greenhouse tomatoes, we detected a new pathogen that forms pseudochains consisting of 12 conidia. To identify the original plant that dispersed this pathogen, wild plants infected with powdery mildew were monitored. The pathogen on Japanese mallotus, Mallotus japonicus, produced a similar type of pseudochain, and conidia were infectious to tomatoes. Inversely, the conidia on the tomato leaves infected M. japonicus. Infectivity assays and internal transcribed spacer (ITS)-based phylogenetic analyses indicated that the two pathogens on the tomato and M. japonicus were identical. These results suggest that the conidia on M. japonicus can be transmitted to greenhouse tomatoes. This work documents the ecological transmission of conidia between wild plants and greenhouse tomatoes.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Nonomura¹,

Matsuda²,

Yamashita³

et al. 2013

Plant Prot. Sci.

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“…A physical method of excluding airborne fungal pathogens and flying pests from greenhouses with good air penetration involves forming an electrostatic barrier; this method reduces the use of agrochemicals such as fungicides and insecticides. The electrostatic method was initially devised to collect mature conidia on powdery mildew conidiophores (Moriura et al, 2006a(Moriura et al, , 2006bNonomura et al, 2009), and the first electrostatic spore precipitator was a screen that created a nonuniform electric field around insulated copper conductor wires arranged in parallel (Matsuda et al, 2006;Shimizu et al, 2007). This field generated an electrostatic force that attracted fungal conidia.…”

Section: Introductionmentioning

confidence: 99%

Prevention of Whitefly Entry from a Greenhouse Entrance by Furnishing an Airflow-Oriented Pre-Entrance Room Guarded with Electric Field Screens

Nonomura¹,

Matsuda²,

Kakutani³

et al. 2014

JAS

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Two types of an electric field screen were used to exclude whiteflies from a greenhouse. Singly charged dipolar electric field screen had insulated conductor iron wires arrayed in parallel (ICW-layer), two earthed metal nets on both sides of the ICW-layer, and a direct current voltage generator. Screens were attached to the lateral windows of the greenhouse to repel whiteflies that approached the nets. To electrostatically guard the greenhouse entrance, doubly charged dipolar electric field screens (DD-screens) were used to capture whiteflies entering through the door. The ICWs, oppositely charged with equal voltages, were arrayed one after the other, and an insulator board or net was placed on one side of the ICW-layer. ICWs captured whiteflies entering an electric field of DD-screens. A small pre-entrance room was constructed at the entrance area, and three DD-screens (with yellow and gray board or gray net) were installed in the pre-entrance room taking into consideration the airflow inside the room, as most whiteflies were brought in by the air when the door was opened. Two DD-screens with the board were useful for directing the wind toward the wall into which the netted DD-screen was integrated. Insects were eliminated by this screen, and the pest-free air was circulated inside the greenhouse. The yellow-boarded DD-screen was highly attractive because of the photo-selective movement of the whiteflies and was effective for trapping the whiteflies when there was no wind. Our electrostatic method is effective at keeping the greenhouse pest-free throughout the entire period of tomato cultivation.

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“…We targeted conidiophores for digital microscopic specification because conidial pseudochains on conidiophores can easily be detected (Oichi et al, 2006). Pseudochain formation was common in the tomato powdery mildew pathogens (Jones et al, 2001;Kiss et al, 2001), and the number of constituent conidial cells in the pseudochains were inherited by the progenies (Nonomura et al, 2009;Oichi et al, 2006). …”

Section: 関西病虫研報（56）：17-20（2014）原著論文mentioning

confidence: 99%

Successional Changes in Powdery Mildew Pathogens Prevailing in Common and Wild Tomato Plants Rotation-cultivated in a Greenhouse

Nonomura

Matsuda

Takikawa

et al. 2014

Kansai Byochugai Kenkyukaiho

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Collection of highly germinative pseudochain conidia of Oidium neolycopersici from conidiophores by electrostatic attraction

Cited by 37 publications

References 24 publications

Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Prevention of Whitefly Entry from a Greenhouse Entrance by Furnishing an Airflow-Oriented Pre-Entrance Room Guarded with Electric Field Screens

Successional Changes in Powdery Mildew Pathogens Prevailing in Common and Wild Tomato Plants Rotation-cultivated in a Greenhouse

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