Collection and characterization of semen in Mithun (Bos frontalis) bulls

Semen Quality Parameters of Freezable and Non-Freezable Ejaculates of Mithun (Bos frontalis) Bulls

“…Mass activity of the ejaculates was positively correlated (p< 0.05) with sperm motility, liveability, intactness of acrosome, nuclear integrity and vanguard distance travelled by sperm and negatively correlated (p< 0.05) with total morphological abnormality in freezable ejaculates (Table 2) whereas in non-freezable ejaculates it was positively correlated (p< 0.05) with sperm viability and integrity of plasma membrane (Table 3). The present observation was similar with report submitted by Karunakaran et al (2007) and Bhattacharyya et al (2009). However, Bhattacharyya et al (2005) reported lower than present value.…”

“…But average values of concentrations in both the groups were well within the range of overall sperm concentration in the present experiment. Similar observation was also reported by Bhattacharyya et al (2005) and Karunakaran et al (2007) in massage method of semen collection, but higher value was observed in artificial vagina method of semen collection (Bhattacharyya et al, 2009;Kumar and Bhattacharyya, 2009;Mondal et al, 2010) in the Mithun bulls. Similar reports as freezable ejaculates have higher sperm concentration than non-freezable ejaculates as reported in bovine species (Loyi, 2008;Gebreselassie, 2009;Gebreselassie et al, 2012).…”

“…Volume of ejaculate in the present study was higher than earlier reports by Bhattacharyya et al (2005) and Karunakaran et al (2007). But volume was lower than semen was collected through artificial vagina method of semen collection in Mithun (Bhattacharyya et al, 2009;Kumar and Bhattacharyya, 2009;Mondal et al, 2010). The results of present study were lower than to those reported by Belorkar et al (1988), Verma (1997), Mohanty (1999) and Loyi (2008) in cattle.…”

“…But some reports were available in Mithun, as forward progressive motility of sperm was 67.74 ± 2.44 (Bhattacharyya et al, 2005) and 75.30 ± 3.50 (Karunakaran et al, 2007) through rectal massage method and through artificial vagina method, it was 78.60 ± 2.60%, (Bhattacharyya et al, 2009), 55.00 ± 8.83% (Kumar and Bhattacharyya, 2009), 82% (Mondal et al, 2010). Similar observation was reported in cattle as freezable ejaculates have higher individual motility than non-freezable ejaculates (Mohanty, 1999;Gebreselassie et al, 2012).…”

“…But the viability percent difference between these two groups has so far not been studied in this bovine species. In Mithun species, percentage of sperm viability through rectal massage method was reported as 68.53 ± 1.85 (Bhattacharyya et al, 2005), 80.6 ± 4.1 (Karunakaran et al, 2007) and through artificial vagina method, it was 80.7 ± 2.2, (Bhattacharyya et al, 2009), 71.73 ± 3.45 (Kumar andBhattacharyya, 2009) and 98 ± 9 (Mondal et al, 2010). Similar observation as freezable ejaculates has significantly higher live sperm percentage than in non-freezable ejaculates as reported in cattle (Gebreselassie et al, 2012;Loyi, 2008).…”