Collecting Nodules and Isolating Rhizobia

Somasegaran, Padma; Hoben, Heinz J.

doi:10.1007/978-1-4613-8375-8_1

Cited by 16 publications

(11 citation statements)

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“…The rhizobia were extracted from the root nodules and cultured in yeast mannitol agar (YEMA) plates containing Congo red dye using the method of Somasegaran and Hoben, (1994). Repeated streaking and a Gram test were also carried out on the isolates to ascertain them as pure colonies (Somasegaran and Hoben, 1994).…”

Section: Extraction Of Rhizobiamentioning

confidence: 99%

“…Repeated streaking and a Gram test were also carried out on the isolates to ascertain them as pure colonies (Somasegaran and Hoben, 1994).…”

Section: Extraction Of Rhizobiamentioning

confidence: 99%

“…Optical density was measured at 600 nm after every three hours using a spectrophotometer. The logarithmic phase of growth was then used to calculate the growth rate (Somasegaran and Hoben, 1994). Isolates were also grown in bromothymol blue (BTB) media.…”

Section: Growth Characteristicsmentioning

confidence: 99%

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Phenotypic characterisation of rhizobia nodulating differentmorphotypes of Vachellia karroo

Ngwenya

Zharare

Basson

et al. 2016

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Vachellia karroo is an important pasture legume which fixes nitrogen through its symbiotic relationship with rhizobia. Thirty two indigenous rhizobia were isolated from four different morphotypes of V. karroo obtained from four agroecologically diverse areas of South Africa. Phenotypic characterization of isolates revealed that they have the same colony morphology. Numeric analysis of the isolates' phenotypic characteristics produced six clusters at 83% level of relative similarity. High variation of the isolates growth in different temperatures, salt concentration, pH, antibiotic resistance and heavy metal resistance was also noted. Carbon source utilization was generally the same for all isolates. Isolates that tolerated a wide range of test conditions are recommended as potentially highly competitive V. karroo inoculants for use in various climatic and soil conditions.

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Section: Extraction Of Rhizobiamentioning

confidence: 99%

“…Repeated streaking and a Gram test were also carried out on the isolates to ascertain them as pure colonies (Somasegaran and Hoben, 1994).…”

Section: Extraction Of Rhizobiamentioning

confidence: 99%

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Phenotypic characterisation of rhizobia nodulating differentmorphotypes of Vachellia karroo

Ngwenya

Zharare

Basson

et al. 2016

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“…Following serial dilution agar plate techniquebacterial isolation was carried out 5,6 .After that these plates were incubated at 28±1ºC and observed daily. Bacterial colonies appeared after 2-3 days were picked up and streaked on YEMA plates.…”

Section: Isolation Of Rhizobiamentioning

confidence: 99%

Biodiversity of Rhizospheric Soil Bacteria and Arbuscular Mycorrhizal (AM) Fungi in Some of The Wild Medicinal Legumes of Barak Valley

Singha¹,

Sharma²,

BilaspurVishwavidyalaya³

2013

Curr World Environ

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“…The nodules were repeatedly washed with sterilized distilled water to get rid of the sterilizing agent. Following serial dilution agar plate technique as described by Somasegaran and Hoben (1994) using yeast extract mannitol agar (YEMA) medium containing 0·0025% Congo red dye (Vincent 1970), bacterial isolations was carried out. After that these plates were incubated at 28 ± 1°C and observed daily.…”

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confidence: 99%

Novel root nodule bacteria belonging to the genus Caulobacter

Edulamudi

Johnson

Divi

et al. 2011

Letters in Applied Microbiology

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Aims: Aim of this study is to determine the genetic variation of rhizobia associated with horse gram [Macrotyloma uniflorum (Lam.) Verdc.] plants grown in different regions of Andhra Pradesh, India. Methods and Results: Four representative isolates having most representative characters from the previous characterization were selected for 16S rRNA sequence. The sequences were submitted to the NCBI GenBank and Ribosomal Database Project (RDP). The isolates HGR‐4, 6 and 13 showed more than 99% homology between them and they were grouped with Rhizobium reference strains where as the isolate HGR‐25 showed 87·1, 87·4 and 87·2% homology with the isolates HGR‐4, 6 and 13, respectively, and were grouped with reference strains for Caulobacter. The nodulation ability of these isolates on horse gram was confirmed by inoculation tests. Conclusions: The isolate HGR‐25 was identified as Caulobacter isolated from the plants growing in soil samples collected from Khareemnagar district, Andhra Pradesh, India. Inoculation tests revealed that Caulobacter formed nodules on horse gram. It was also confirmed by RDP. Significance and Impact of the Study: This is the first report that a legume was nodulated by a member of the genus Caulobacter, which belongs to the family Caulobacteriaceae in the order Caulobacterales of Alphaproteobacteria.

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Collecting Nodules and Isolating Rhizobia

Cited by 16 publications

References 1 publication

Phenotypic characterisation of rhizobia nodulating differentmorphotypes of Vachellia karroo

Phenotypic characterisation of rhizobia nodulating differentmorphotypes of Vachellia karroo

Biodiversity of Rhizospheric Soil Bacteria and Arbuscular Mycorrhizal (AM) Fungi in Some of The Wild Medicinal Legumes of Barak Valley

Novel root nodule bacteria belonging to the genus Caulobacter

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