Collecting and Processing Cacti into Herbarium Specimens, Using Ethanol and Other Methods

Groot, Sarah J. De

doi:10.1600/036364411x604985

Cited by 10 publications

(9 citation statements)

References 4 publications

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“…The collection sites include deserted dry desert areas of district Attock and Mianwali (Figure 1). The plant were dried by leaving the stem sections in shade or slicing the specimen to removing the inner parenchyma and then mounted on herbarium sheets (Groot, 2011).…”

Section: Methodsmentioning

confidence: 99%

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Morpho‐palynological and anatomical studies in desert cacti (Opuntia dillenii and Opuntia monacantha) using light and scanning electron microscopy

Majeed

Zafar

Ahmad

et al. 2022

Microscopy Res & Technique

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Opuntia is the most diverse and widely distributed drought resistant promising genus of family Cactaceae. The cladodes were utilized to quantify the chemical composition of these plants helpful in lignocellulose conversion and their application towards biofuel production. The present study was aimed to evaluate and compare the taxonomic relationship based on morphology, stem anatomy and palynology of important desert cacti including Opuntia dillenii and Opuntia monacantha. This study also evaluates the potential usefulness of morphological, anatomical and pollen traits using light and scanning electron microscopy. The obtained microcharacters of stem and flowers are considered diagnostic at the generic and specific levels. Some distinguishing morphological features observed were elliptical to obovate cladodes, 1-7 spines per areole and presence of glochidia in O. dillenii. Pollen and stem anatomical characters of the studied taxa are considered highly diagnostic at the generic and species levels. The epidermis has irregular and wavy cells, with straight to sinuate wall pattern and paracytic stomata. Pollen grains appear as pantoporate and prolate spheroidal having reticulate to perforate-reticulate sculpturing while exine semi-tectate to tectate. The taxonomic features studied could be valuable to elaborate and helpful in correctly identification of Opuntia species. The methods of diverse microscopic examination also providing sufficient evidence about the taxonomy of the Opuntia species. Research Highlights• Description and illustration of desert cacti Opuntia.• Morpho-anatomy and palynology were studied with LM and SEM.• Highly variation in taxonomic qualitative and quantitative features.• Systematic significance based on taxonomic characters was presented.

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Section: Methodsmentioning

confidence: 99%

“…(Figure 1). The plant were dried by leaving the stem sections in shade or slicing the specimen to removing the inner parenchyma and then mounted on herbarium sheets (Groot, 2011).…”

Section: The Anatomy Of Different Genera Of Cactaceae Such As Arrojad...mentioning

confidence: 99%

Morpho‐palynological and anatomical studies in desert cacti (Opuntia dillenii and Opuntia monacantha) using light and scanning electron microscopy

Majeed

Zafar

Ahmad

et al. 2022

Microscopy Res & Technique

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“…O material foi herborizado segundo Groot (2011) e depositados no Herbário VIES, da Universidade Federal do Espírito Santo. As identificações foram realizadas mediante literatura especializada (Barthlott;Taylor, 1995;Taylor;Zappi, 2004;Calvente, 2010).…”

Section: Methodsunclassified

Cactaceae em um fragmento florestal urbano de Vila Velha, Espírito Santo, Brasil

Cardoso¹,

Valadares

Thomaz

et al. 2020

Paubrasilia

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Cactaceae é uma família quase que exclusivamente endêmica do Novo Mundo. No estado do Espírito Santo, Brasil, a família é pouco amostrada e inventários florísticos de seus representantes são escassos. O objetivo deste trabalho foi realizar a flora das espécies de Cactaceae ocorrentes em um fragmento urbano antropizado na Floresta Atlântica no Espírito Santo. Foram realizadas excursões quinzenais no período entre agosto de 2011 e julho de 2012. Nove espécies de Cactaceae foram encontradas, pertencentes a sete gêneros. Três das espécies são endêmicas da Floresta Atlântica e duas são citadas como ameaçadas de extinção, uma delas registrada para o Espírito Santo pela primeira vez, após uma lacuna aproximada de um século. Nossos dados ressaltam a importância da conservação do fragmento florestal e a necessidade de estudos acerca da família em outras áreas do estado. Apresentamos uma chave taxo-nômica ilustrada, descrições, comentários sobre a taxonomia, distribuição geográfica e ecologia das espécies, além da conservação de suas populações na área.

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“…Field trips to the CMR were carried out between November 2013 and February 2016, and some additional collections were made after this time. The material collected was processed according to the methodology in Groot (2011) and deposited in the R and VIES herbaria (acronyms according to Thiers, continuously updated). The CVRD, MBML, R, RB, SAMES, SPF, UPCB and VIES collections were also visited to examine specimens relevant to this research.…”

Section: Biological Materialsmentioning

confidence: 99%

Cactaceae in a priority area for conservation in Espírito Santo state

et al. 2022

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The Central Mountain Region of Espírito Santo state is located in the Atlantic Forest domain in eastern Brazil, which is the third largest center of diversity and endemism for Cactaceae. The state has 42 native species and 13 genera of this family. The aim of this study was to conduct a floristic-taxonomic study of Cactaceae in the Central Mountain Region of Espírito Santo, an area of extreme priority for conservation of Atlantic Forest biodiversity. Field trips were carried out between November 2013 and February 2016, with some additional collections and herbarium specimens were assessed. Twenty-two native species of Cactaceae were recorded for the region, which belong to the genera Coleocephalocereus (2), Epiphyllum (1), Hatiora (2), Lepismium (2), Pereskia (1), Rhipsalis (12), Schlumbergera (1) and Selenicereus (1). Of these species, 18 are epiphytes, 12 are rupicolous and 2 are climbers, some of them exhibiting more than one life-form. Two species are endemic to the state and eleven are assessed as threatened species regionally. This study contributes to improve what is known about Cactaceae in Espírito Santo state.

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Collecting and Processing Cacti into Herbarium Specimens, Using Ethanol and Other Methods

Cited by 10 publications

References 4 publications

Morpho‐palynological and anatomical studies in desert cacti (Opuntia dillenii and Opuntia monacantha) using light and scanning electron microscopy

Morpho‐palynological and anatomical studies in desert cacti (Opuntia dillenii and Opuntia monacantha) using light and scanning electron microscopy

Cactaceae em um fragmento florestal urbano de Vila Velha, Espírito Santo, Brasil

Cactaceae in a priority area for conservation in Espírito Santo state

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