Collecting and Culturing Lineus sanguineus to Study Nemertea WBR

Zattara, Eduardo E.; Fernández-Álvarez, Fernando Ángel

doi:10.1007/978-1-0716-2172-1_12

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…Within Spiralia, larval regeneration has been examined in one nemertean species. Similar to annelids, nemertean adult regeneration is variable (Zattara et al, 2019; Zattara & Fernández‐Alvarez, 2022). In early stage larvae of Maculaura alaskensis , there appears to be high regenerative potential, which progressively decreases with maturation until it stabilizes in adults capable of posterior regeneration (Hiebert & Maslakova, 2015).…”

Section: Discussionmentioning

confidence: 99%

Investigating the developmental onset of regenerative potential in the annelid Capitella teleta

Boyd,

Seaver

2023

Invertebrate Biology

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An animal's ability to regrow lost tissues or structures can vary greatly during its life cycle. The annelid Capitella teleta exhibits posterior, but not anterior, regeneration as juveniles and adults. In contrast, embryos display only limited replacement of specific tissues. To investigate when during development individuals of C. teleta become capable of regeneration, we assessed the extent to which larvae can regenerate. We hypothesized that larvae exhibit intermediate regeneration potential and demonstrate some features of juvenile regeneration, but do not successfully replace all lost structures. Both anterior and posterior regeneration potential of larvae were evaluated following amputation. We used several methods to analyze wound sites: EdU incorporation to assess cell proliferation; in situ hybridization to assess stem cell and differentiation marker expression; immunohistochemistry and phalloidin staining to determine presence of neurites and muscle fibers, respectively; and observation to assess re‐epithelialization and determine regrowth of structures. Wound healing occurred within 6 h of amputation for both anterior and posterior amputations. Cell proliferation at both wound sites was observed for up to 7 days following amputation. In addition, the stem cell marker vasa was expressed at anterior and posterior wound sites. However, growth of new tissue was observed only in posterior amputations. Neurites from the ventral nerve cord were also observed at posterior wound sites. De novo ash expression in the ectoderm of anterior wound sites indicated neuronal cell specification, although the absence of elav expression indicated an inability to progress to neuronal differentiation. In rare instances, cilia and eyes re‐formed. Both amputations induced expanded expression of the myogenesis gene MyoD in preexisting tissues. Our results indicate that amputated larvae complete early, but not late, stages of regeneration, which indicates a gradual acquisition of regenerative ability in C. teleta. Furthermore, amputated larvae can metamorphose into burrowing juveniles, including those missing brain and anterior sensory structures. To our knowledge, this is the first study to assess regenerative potential of annelid larvae.

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Section: Discussionmentioning

confidence: 99%

Investigating the developmental onset of regenerative potential in the annelid Capitella teleta

Boyd,

Seaver

2023

Invertebrate Biology

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Investigating the developmental onset of regenerative potential in the annelidCapitella teleta

Boyd

Seaver

2023

Preprint

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An animal's ability to regrow lost tissues or structures can vary greatly during its life cycle. The annelid Capitella teleta has robust posterior, but not anterior, regeneration as a juvenile and an adult. In contrast, embryos display only limited replacement of specific tissues. To investigate when during development C. teleta becomes capable of regeneration, we assessed the extent to which larvae can regenerate. We hypothesized that larvae exhibit intermediate regeneration potential and demonstrate some features of juvenile regeneration, but do not successfully replace all lost structures. Both anterior and posterior regeneration potential of larvae were evaluated following amputation. Responses were analyzed by EdU incorporation, immunohistochemistry, phalloidin staining, and in situ hybridization. Wound sites were evaluated for re-epithelialization, cell proliferation, stem cell marker expression, the presence of neurites and regrowth of structures. Wound healing occurred within 6 hours of amputation. Cell proliferation at the wound site was observed for up to 7 days following amputation. In addition, the stem cell marker vasa was expressed at anterior and posterior wound sites. However, growth of new tissue was observed only in posterior amputations. Neurites from the ventral nerve cord were also observed at posterior wound sites. De novo ash expression in the ectoderm of anterior wound sites indicated neuronal cell specification, although the absence of elav expression indicated an inability to progress to neuronal differentiation. In rare instances, cilia and eyes reformed. Both amputations induced ectopic expression of the myogenesis gene myoD. Our results indicate that amputated larvae complete early, but not late, stages of regeneration, indicating a gradual acquisition of regenerative ability in C. teleta. Furthermore, amputated larvae can metamorphose into burrowing juveniles, including those missing brain and anterior sensory structures. To our knowledge, this is the first study to assess regenerative potential of annelid larvae.

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Collecting and Culturing Lineus sanguineus to Study Nemertea WBR

Cited by 2 publications

References 21 publications

Investigating the developmental onset of regenerative potential in the annelid Capitella teleta

Investigating the developmental onset of regenerative potential in the annelid Capitella teleta

Investigating the developmental onset of regenerative potential in the annelidCapitella teleta

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