Collapsin Response Mediator Protein-1 and the Invasion and Metastasis of Cancer Cells

Shih, Jin‐Yuan; Yang, Shuenn Chen; Hong, Tse-Ming; Yuan, Ang; Chen, Jeremy J.W.; Yu, Chong Jen; Chang, Yih‐Leong; Lee, Yung Chie; Peck, Konan; Wu, Cheng Wen; Yang, Pan Chyr

doi:10.1093/jnci/93.18.1392

Cited by 145 publications

(149 citation statements)

References 58 publications

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…17,18 As shown in Figure 1A, expression of caveolin-1 protein in the low-invasive CL1-0 and CL1-1 cells was undetectable. In contrast, it was abundantly expressed in the highly invasive CL1-5 and CL1-5F4 cells.…”

Section: Elevated Caveolin-1 Expression In CL Cells Correlated With Ementioning

confidence: 94%

Up-Regulated Caveolin-1 Accentuates the Metastasis Capability of Lung Adenocarcinoma by Inducing Filopodia Formation

Huang

et al. 2002

The American Journal of Pathology

207

154

View full text Add to dashboard Cite

Caveolin-1, a 21-to 24-kd protein, is the principal component of caveolae, which are special invaginated microdomains of the plasma membrane present in most mammalian cells. 1 It is well established that caveolin-1 is a tumor suppressor gene. Caveolin-1 mRNA and protein expression are frequently lost in human cancer cell lines. Re-expression of caveolin-1 in oncogenically transformed cell lines inhibits tumor cell growth and reduces tumorigenicity. [2][3][4][5][6] Several mechanisms have been proposed for caveolin-1 to function as a tumor suppressor. Caveolin-1 may exert its tumor-growth inhibition by contact inactivation of signaling molecules such as v-src, Ha-Ras, protein kinase A, PKC, and p42/44 MAP kinase within caveolae. [7][8][9][10] In addition, down-regulation of caveolin-1 in colon carcinoma cells has been shown to prevent the degradation of inducible nitric oxide synthase via the proteosome pathway, which, in turn, increases the local nitric oxide concentration to facilitate tumorigenesis. 11 Caveolin-1 can also function as a tumor metastasispromoting molecule, which is unrelated to its obvious function of cell growth inhibition. 12 Elevated expression of caveolin-1 is found to be associated with progression of prostate, colon, and breast carcinoma. 13,14 Inhibition of c-myc-induced apoptosis by caveolin-1 was recently proposed to promote progression of prostate cancer, 15 and it may serve as a prognostic indicator for these patients. 16 Nonetheless, it still remains unclear whether and how caveolin-1 can potentiate tumor progression in other types of human cancer.In the present study, we investigated the expression pattern of caveolin-1 both in a series of lung carcinoma cell lines (CLs) with varying invasive/metastatic ability 17

show abstract

Section: Elevated Caveolin-1 Expression In CL Cells Correlated With Ementioning

confidence: 94%

Up-Regulated Caveolin-1 Accentuates the Metastasis Capability of Lung Adenocarcinoma by Inducing Filopodia Formation

Huang

et al. 2002

The American Journal of Pathology

207

154

View full text Add to dashboard Cite

show abstract

“…The specific rabbit anti-human anti-LCRMP-1 polyclonal antibody (C2) for western blotting and IHC was produced by the authors using a synthetic peptide derived from the unique N-terminal region of LCRMP-1, as described previously (6). Formalin-fixed, paraffin-embedded sections (5-µm thick) were processed for IHC.…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

“…CRMP-1 has been observed to be a cancer invasion suppressor (6). In addition, a negative association between CRMP-1 expression and cancer cell invasiveness has been observed in non-small cell lung cancer (NSCLC) patients (7).…”

Section: Introductionmentioning

confidence: 99%

Long form collapsin response mediator protein-1 promotes the migration and invasion of osteosarcoma cells

et al. 2016

View full text Add to dashboard Cite

Abstract. It has been reported that long form collapsin response mediator protein-1 (LCRMP-1) promotes the metastasis of non-small cell lung cancer. Osteosarcoma (OS) is a human cancer with a high potential for metastasis. The present study aimed to investigate the role of LCRMP-1 in OS metastasis. The expression of LCRMP-1 in OS specimens and cell lines was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. Furthermore, the migration and invasion of OS cells with LCRMP-1-knockdown was investigated to examine the role of LCRMP-1 in OS metastasis. In addition, the expression of N-cadherin and matrix metalloproteinases (MMPs), which are involved in cell migration, was evaluated using RT-qPCR. Increased expression of LCRMP-1 was observed in the OS tissues and cell lines, accompanied by the enhanced migration and invasion of the OS cells. LCRMP-1-knockdown resulted in a significant decrease in the expression of N-cadherin and MMPs, as well as inhibition of the migration and invasion of the OS cells. Overexpression of LCRMP-1 promoted OS metastasis. Therefore, LCRMP-1 may be a promising target for the effective treatment of OS.

show abstract

“…77,79 Lung cancer cell line A549 was purchased from Bioresource Collection and Research Center (BCRC), Taiwan. A complete medium composed of Dulbecco's Modified Eagle's medium (DMEM, Gibco) and 10% fetal bovine serum (FBS, Invitrogen) was used for all cells.…”

Section: Cell Preparationmentioning

confidence: 99%

“…The size of the chamber and thus the medium consumption are largely reduced. The electrotaxis effect of human lung cell lines, A549, CL1-0, and CL1-5, [77][78][79] in 3D scaffolds was observed and compared with that studied in 2D gelatin-coated and bare substrates. 9 …”

Section: Introductionmentioning

confidence: 99%

Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

et al. 2012

View full text Add to dashboard Cite

In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338 mV/mm through the scaffold for the study of cells' migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate

show abstract

Collapsin Response Mediator Protein-1 and the Invasion and Metastasis of Cancer Cells

Abstract: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

Cited by 145 publications

References 58 publications

Up-Regulated Caveolin-1 Accentuates the Metastasis Capability of Lung Adenocarcinoma by Inducing Filopodia Formation

Up-Regulated Caveolin-1 Accentuates the Metastasis Capability of Lung Adenocarcinoma by Inducing Filopodia Formation

Long form collapsin response mediator protein-1 promotes the migration and invasion of osteosarcoma cells

Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

Contact Info

Product

Resources

About