Collapsed monomolecular thin films as surface nanomodification techniques for bioorganic MALDI analysis

Silyavka, Elena S.; Selyutin, A. A.; Суходолов, Н. Г.; Shilovskikh, Vladimir V.; Oleneva, P. A.; Mitrofanov, Andrei; Keltsieva, Olga A.; Dubakova, P. S.; Babakov, V. N.; Alexandrova, Marina L.; Krasnov, N. V.; Podolskaya, Е. P.

doi:10.1063/1.5087677

Cited by 2 publications

(1 citation statement)

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“…The main advantages of optical polymer fibers in comparison with glass are low weight, low price, and ease of use. , Apropos, recent works have appeared in which monolayers containing ions of transition metals and lanthanides are used as sorption materials. In particular, these works investigate similar materials as adsorbents for bioorganic analysis. − Thin films transferred onto a substrate can act as metal-affinity sorbents. Such sorbents have an advantage because metals with an increased affinity for sites of biologically active macromolecules are accessible.…”

Section: Introductionmentioning

confidence: 99%

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

et al. 2022

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This article presents new possibilities of using thin films of lanthanide stearates as sorbent materials. Modification of the Q-sense device resonator with monolayers of lanthanide stearates by the Langmuir–Schaeffer method made it possible to study the process of insulin protein adsorption on the surface of new thin-film sorbents. The resulting films were also characterized by compression isotherms, chemical analysis, scanning electron microscopy, and mass spectrometry. The transition of stearic acid to salt was recorded by IR spectroscopy. Using the LDI MS method, the main component of thin films, lanthanide distearate, was established. The presence of Eu 2+ in thin films was revealed. In the case of europium stearate, the maximum value of insulin adsorption was obtained, −1.67·10 −10 mole/cm 2 . The findings suggest the possibility of using thin films of lanthanide stearates as a sorption material for the proteomics determination of the quantitative protein content in complex fluid systems by specific adsorption on modified surfaces and isolation of such proteins from complex mixtures.

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Section: Introductionmentioning

confidence: 99%

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

et al. 2022

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Identification of phosphonylated peptides using a MALDI target functionalized with lanthanum stearate

Babakov

Gorbunov

Gladchuk³

et al. 2023

MES

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As markers of intoxication, adducts of blood proteins with organophosphorus compounds (OPs) allow establishing the fact of poisoning and, furthermore, enable identification of the OPs by the attached residue. This study aimed to develop a method of specific and selective extraction of blood protein adducts carrying OPs on the surface of a matrix-assisted laser desorption/ionization (MALDI) target functionalized with multimolecular structures based on lanthanum stearate using metal affinity chromatography. We have shown the ability of adsorbent to retain both full-size and dealkylated adducts of blood proteins with OPs. The developed method allowed extraction and identification of peptides of human serum albumin and human butyrylcholinesterase modified with various OPs (after incubation of human blood plasma with OPs in concentrations from 1 to 100 ng/mL), which makes this approach applicable for the purposes of OPs identification in the context of investigation of real cases of intoxication.

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Collapsed monomolecular thin films as surface nanomodification techniques for bioorganic MALDI analysis

Cited by 2 publications

References 8 publications

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

Thin Films of Lanthanide Stearates as Modifiers of the Q-Sense Device Sensor for Studying Insulin Adsorption

Identification of phosphonylated peptides using a MALDI target functionalized with lanthanum stearate

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