Collapse pressure measurement of single hollow glass microsphere using single-beam acoustic tweezer

Yoo, Jinhee; Kim, Yeong-Geun; Lim, Hae Gyun; Kim, Hyunhee

doi:10.1016/j.ultsonch.2021.105844

Cited by 7 publications

(4 citation statements)

References 87 publications

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“…However, these complex experimental procedures also cause difficulties in obtaining large data sets. A small data set is an intrinsic limitation of the domain of single-cell analysis using acoustic tweezers 56 – 62 , which may lead to overfitting issues and data reliability of experimental results. To address possible issues, we initially conducted 3-fold cross-validation to examine whether the proposed model could deal with the diversity of live cells.…”

Section: Resultsmentioning

confidence: 99%

Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing

Jeon

Lim

Shung

et al. 2022

Sci Rep

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This study aimed to automatically classify live cells based on their cell type by analyzing the patterns of backscattered signals of cells with minimal effect on normal cell physiology and activity. Our previous studies have demonstrated that label-free acoustic sensing using high-frequency ultrasound at a high pulse repetition frequency (PRF) can capture and analyze a single object from a heterogeneous sample. However, eliminating possible errors in the manual setting and time-consuming processes when postprocessing integrated backscattering (IB) coefficients of backscattered signals is crucial. In this study, an automated cell-type classification system that combines a label-free acoustic sensing technique with deep learning-empowered artificial intelligence models is proposed. We applied an one-dimensional (1D) convolutional autoencoder to denoise the signals and conducted data augmentation based on Gaussian noise injection to enhance the robustness of the proposed classification system to noise. Subsequently, denoised backscattered signals were classified into specific cell types using convolutional neural network (CNN) models for three types of signal data representations, including 1D CNN models for waveform and frequency spectrum analysis and two-dimensional (2D) CNN models for spectrogram analysis. We evaluated the proposed system by classifying two types of cells (e.g., RBC and PNT1A) and two types of polystyrene microspheres by analyzing their backscattered signal patterns. We attempted to discover cell physical properties reflected on backscattered signals by controlling experimental variables, such as diameter and structure material. We further evaluated the effectiveness of the neural network models and efficacy of data representations by comparing their accuracy with that of baseline methods. Therefore, the proposed system can be used to classify reliably and precisely several cell types with different intrinsic physical properties for personalized cancer medicine development.

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Section: Resultsmentioning

confidence: 99%

Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing

Jeon

Lim

Shung

et al. 2022

Sci Rep

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“…Additionally, the same UHF transducer was used to demonstrate its capability for trapping single PC-3 cells using an acoustic tweezer technique, without damaging it. Acoustic tweezers are an emerging tool in the field of biomedical and physical research [ 45 , 46 , 47 , 48 ] due to their non-contact and strong radiation forces [ 49 , 50 ]. They have been widely used for single-cell analysis, such as measuring calcium elevation and mechanical properties of cells [ 51 , 52 , 53 , 54 , 55 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis

Jung

Shung

Lim

2023

Sensors

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Ultrasound imaging is a highly valuable tool in imaging human tissues due to its non-invasive and easily accessible nature. Despite advances in the field of ultrasound research, conventional transducers with frequencies lower than 20 MHz face limitations in resolution for cellular applications. To address this challenge, we employed ultrahigh frequency (UHF) transducers and demonstrated their potential applications in the field of biomedical engineering, specifically for cell imaging and acoustic tweezers. The lateral resolution achieved with a 110 MHz UHF transducer was 20 μm, and 6.5 μm with a 410 MHz transducer, which is capable of imaging single cells. The results of our experiments demonstrated the successful imaging of a single PC-3 cell and a 15 μm bead using an acoustic scanning microscope equipped with UHF transducers. Additionally, the dual-mode multifunctional UHF transducer was used to trap and manipulate single cells and beads, highlighting its potential for single-cell studies in areas such as cell deformability and mechanotransduction.

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“…Therefore, they are suitable for in vitro studies and have been used to measure the physical and biological properties of a single particle. 27 , 33 , 34 , 35 SBATs in the Rayleigh regime work when the wavelength is greater than the object’s size. 29 , 32 They work even with longer focal depths and wider beam widths than those in the Mie regime.…”

Section: Introductionmentioning

confidence: 99%

Red blood cell trapping using single-beam acoustic tweezers in the Rayleigh regime

Yoo,

Kim,

Lee

et al. 2023

iScience

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Collapse pressure measurement of single hollow glass microsphere using single-beam acoustic tweezer

Abstract: Graphical abstract

Cited by 7 publications

References 87 publications

Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing

Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing

Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis

Red blood cell trapping using single-beam acoustic tweezers in the Rayleigh regime

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