2009
DOI: 10.1016/j.cell.2009.04.027
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Collapse of Germline piRNAs in the Absence of Argonaute3 Reveals Somatic piRNAs in Flies

Abstract: Summary Piwi-interacting RNAs (piRNAs) silence transposons in the germ line of animals. They are thought to derive from long primary transcripts spanning transposon-rich genomic loci, “piRNA clusters.” piRNAs are proposed to direct an auto-amplification loop in which an antisense piRNA, bound to Aubergine or Piwi protein, directs the cleavage of sense RNA, triggering production of a sense piRNA bound to the PIWI protein Argonaute3 (Ago3). In turn, the new piRNA is envisioned to direct cleavage of a cluster tra… Show more

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Cited by 498 publications
(800 citation statements)
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“…S2), we next looked at the impact of germ-line Piwi depletion on the localization pattern of Aub. Mutations disrupting the ping-pong amplification process can lead to mislocalization of Aub from the peri-nuclear structure nuage (13,34), the proposed site of secondary piRNA production (34). Similar to earlier observations using mutants that disrupt Piwi in both germ line and soma (14), we found no impact on Aub localization to the nuage (Fig.…”
Section: Resultssupporting
confidence: 90%
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“…S2), we next looked at the impact of germ-line Piwi depletion on the localization pattern of Aub. Mutations disrupting the ping-pong amplification process can lead to mislocalization of Aub from the peri-nuclear structure nuage (13,34), the proposed site of secondary piRNA production (34). Similar to earlier observations using mutants that disrupt Piwi in both germ line and soma (14), we found no impact on Aub localization to the nuage (Fig.…”
Section: Resultssupporting
confidence: 90%
“…Telomeric retrotransposon HeT-A and LTR retrotransposon Burdock show the most dramatic effects. In general, transposons that show increased expression in germ-line Piwi knockdown lines were the same as those that showed increased expression in an ago3 mutant line as reported in Li et al (13) (Table 1). This correlation argues that germ-line Piwi functions in the same pathway as Aub and AGO3 (8,9).…”
Section: Resultssupporting
confidence: 73%
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