Collagenous Microbeads as a Scaffold for Tissue Engineering with Adipose-Derived Stem Cells

Abstract

Collagenous microbeads are a favorable scaffold for adipose-derived stem cells, allowing ex vivo proliferation and differentiation on particles that are small enough to be injected.

A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells

“…Similarly to 2D culture, EADSC grown and differentiated in 3D culture retained high cell viability ([94 %), as seen previously (Cheng et al 2012;Rubin et al 2007). In 2D culture, a decrease in cell viability was observed following osteogenic differentiation, potentially due to the formation of the cells into calcified nodules, however in 3D culture, cell viability remained constant.…”

Regenerative Therapy After Cancer: What Are the Risks?

“…The rationale for combining whole fat or lipoaspirate with SVF cells or ASC is that the organized fat tissue may serve as a scaffold upon which more concentrated stem/progenitor cells can organize and differentiate. Another attractive approach for guiding the three-dimensional organization of engineered tissue reconstruction is through the use of scaffolding, [22][23][24] which may be mineral, synthetic polymers, or biological and may incorporate growth factors naturally 25 or by design.…”

Tissue Engineering Generation of adipose tissue: an overview of current standards and possibilities

“…Isolation of the cells from adipose tissue is achieved by digestion supported by a collagen solution, repeated filtration, and centrifugation. The cell fraction in the remaining cell pellet is subsequently cultivated, then proliferation or differentiation is stimulated [61][62][63]. It must be considered though that depending on age, sex, and localization, adipose tissue can show different endocrinologic activity, which can affect the proliferation and differentiation performance of the harvested stem cells [64,65].…”