Collageneous matrix coatings on titanium implants modified with decorin and chondroitin sulfate: Characterization and influence on osteoblastic cells

Abstract

Studies in developmental and cell biology have established the fact that responses of cells are influenced to a large degree by morphology and composition of the extracellular matrix. Goal of this work is to use this basic principle to improve the biological acceptance of implants by modifying the surfaces with components of the extracellular matrix (ECM), utilizing the natural self-assembly potential of collagen in combination with further ECM components in close analogy to the situation in vivo. Aiming at load-bearing applications in bone contact, collagen type I in combination with the proteoglycan decorin and the glycosaminoglycan chondroitin sulfate (CS) was used; fibrillogenesis, fibril morphology, and adsorption of differently composed fibrils onto titanium were assessed. Both decorin and CS could be integrated into the fibrils during fibrillogenesis, the amount bound respectively desorbed depending on the ionic strength of fibrillogenesis buffer. Including decorin always resulted in a significant decrease of fibril diameter, CS in only a slight decrease or even increase, depending on the collagen preparation used. No significant changes in adsorption to titanium could be detected. Osteoblastic cells showed different reactions for cytoskeletal arrangement and osteopontin expression depending on the composition of the ECM, with CS enhancing the osteoblast phenotype.

Regenerative potential of glycosaminoglycans for skin and bone

“…Immobilized monosulfated HA facilitated the proliferation and TNAP activity of human osteoblasts in the same manner as a coating with collagen [97]. Implant coating with collagen fibrils and CS or CS-PGs promotes adhesion and differentiation of osteoblasts and osseointegration [87,[107][108][109][110][111][112].…”

Expression of Versican Isoform V3 in the Absence of Ascorbate Improves Elastogenesis in Engineered Vascular Constructs

“…29 There is recent evidence that the inhibiting effect of GAGs on collagen fibril thickness is mediated by proteoglycan-bound GAG (e.g., decorin) and not by free GAG. 32 GAGs and CSproteoglycans may also increase matrix metalloproteinase activity. 33 Correspondingly, the increased concentration of GAGs that we observed in the control LX vascular constructs relative to the LV3 constructs was associated with thinner collagen fibril bundles and a lower content of elastin and elastin-associated proteins which may have contributed to the LX constructs being structurally weaker than the LV3 constructs.…”

Biocompatibility and Anti-Microbiological Activity Characterization of Novel Coatings for Dental Implants: A Primer for Non-Biologists

“…The establishment of This makes them difficult and expensive to work with; however, the results are much closer to the in vivo situation. Often used cell types are immortalized neonatal mouse osteoblasts (MC3T3E1), human osteosarcoma (SaOS-2 or MG-63), primary rat calvaria cells, or L929 mouse fibroblasts (Meyer et al, 2005;Monsees et al, 2005;Bierbaum et al, 2006;Subramanian et al, 2015).…”