Collagenase production by early and late passage cultures of human fibroblasts

Millis, Albert J.T.; Sottile, Jane; Hoyle, Marian; Mann, David; Diemer, Verena

doi:10.1016/0531-5565(89)90060-0

Cited by 45 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Collagen, the major structural component of the skin in the dermis has been suggested to be the cause of the clinical changes observed in naturally aged and photo-aged skin (Millis et al, 1989;Chung et al, 2001). Collagen synthesis has been studied by serial cultures of human dermal fibroblasts (Millis et al, 1989). The effects on skin collagen synthesis for anti-aging evaluation can be determined from the proliferation activity of the human fibroblasts by the sulforhodamine B (SRB) assay.…”

Section: Introductionmentioning

confidence: 99%

“…Fibroblasts which produce collagens, glycosaminoglycans, reticular and elastic fibers, and glycoproteins are found in the extracellular matrix. Collagen, the major structural component of the skin in the dermis has been suggested to be the cause of the clinical changes observed in naturally aged and photo-aged skin (Millis et al, 1989;Chung et al, 2001). Collagen synthesis has been studied by serial cultures of human dermal fibroblasts (Millis et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitroanti-aging activities ofTerminalia chebulagall extract

Manosroi¹,

Jantrawut²,

Akihisa³

et al. 2010

Pharmaceutical Biology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitroanti-aging activities ofTerminalia chebulagall extract

Manosroi¹,

Jantrawut²,

Akihisa³

et al. 2010

Pharmaceutical Biology

View full text Add to dashboard Cite

“…Fibroblasts from skin that is at risk for the development of nonhealing ulcers demonstrate impaired proliferative capacity in vitro (11)(12)(13). Reduced fibroblast growth is associated with reduced collagen synthesis (14 -16) and increased matrix metalloproteinase (MMP) production (14,(17)(18)(19).…”

mentioning

confidence: 99%

All-trans Retinoic Acid Improves Structure and Function of Diabetic Rat Skin in Organ Culture

et al. 2002

View full text Add to dashboard Cite

Diabetes increases susceptibility to chronic ulceration. The cause of chronic wound formation in diabetic individuals is multifactorial but may be accelerated by changes in the structure and function of the skin secondary to impaired fibroblast proliferation, decreased collagen synthesis, and increased matrix metalloproteinase (MMP) expression. This study explored cellular and biochemical changes in organ cultures of skin from streptozotocin-diabetic (STZ-D) rats and the effects of all-trans retinoic acid (RA) on these changes. STZ-D rats were killed after 6 weeks. The skin was cut into 2-mm pieces and incubated in organ culture for 3 or 6 days in the absence or presence of 3 mol/l RA. After organ culture incubation, control and RA-treated tissue was examined histologically after staining with hematoxylin and eosin. In parallel, organ culture-conditioned medium was assayed for MMPs. Additional organ cultures were examined for collagen synthesis using 3 H-proline incorporation into trichloroacetic acid-precipitable material and for glycosaminoglycan production based on interaction with the cationic dye 1,9-dimethylmethylene blue and by staining of tissue sections with periodic acid Schiff reagents. Skin from 6-week STZ-D rats demonstrated features of dermal atrophy including thinning and disorganization of connective tissue bundles and increased space between bundles. The addition of RA resulted in cellular reactivation and partially reversed the histological features of dermal atrophy. Levels of latent and active MMP-9 and MMP-13 were elevated 4-and 10-fold, respectively, in STZ-D skin and reduced by 50 -75% (P < 0.05) by RA. Collagen synthesis was increased by 30% (P < 0.05) by RA, whereas glycosaminoglycan expression was increased by only 9% (NS). RA also increased proliferation of STZ-D skin fibroblasts (approximately threefold over control; P < 0.05). Together, these data suggest that RA has the capacity to improve structure and function of diabetic skin. Diabetes 51: 3510 -3516, 2002

show abstract

“…Examples of genes whose expression is elevated in senescent cells are those for p16 INK4 , p21 CIP1 , cyclins D1 and E, APP, endothelin-1, fibronectin, interleukin-1, Cu, ZnSOD, GADD45, PAI-1, PAI-2, SPARC, IGFBP-3, collagenase, macrophage colony-stimulating factor, p53, bcl-2, and p33 ING1 . Examples of genes whose expression is reduced in senescent cells are those for cyclin A, cyclin B1, cyclin H, CAK, cdc2, MnSOD, c-fos, catalase, EPC-1, E2F-1, E2F-2, DP-1, elastin, thymidine kinase, IGF-II, egr-1, granulocyte-macrophage colony-stimulating factor, dihydrofolate reductase, PCNA, ribonucleotide reductase, and histones (2,16,21,22,26,31,33; for reviews, see references 4, 5, 14, 32, and 37).…”

mentioning

confidence: 99%

Loss of HuR Is Linked to Reduced Expression of Proliferative Genes during Replicative Senescence

Wang

Yang

Cristofalo

et al. 2001

Molecular and Cellular Biology

168

160

View full text Add to dashboard Cite

Cellular aging is accompanied by alterations in gene expression patterns. Here, using two models of replicative senescence, we describe the influence of the RNA-binding protein HuR in regulating the expression of several genes whose expression decreases during senescence. We demonstrate that HuR levels, HuR binding to target mRNAs encoding proliferative genes, and the half-lives of such mRNAs are lower in senescent cells. Importantly, overexpression of HuR in senescent cells restored a "younger" phenotype, while a reduction in HuR expression accentuated the senescent phenotype. Our studies highlight a critical role for HuR during the process of replicative senescence.

show abstract

Collagenase production by early and late passage cultures of human fibroblasts

Cited by 45 publications

References 35 publications

In vitroanti-aging activities ofTerminalia chebulagall extract

In vitroanti-aging activities ofTerminalia chebulagall extract

All-trans Retinoic Acid Improves Structure and Function of Diabetic Rat Skin in Organ Culture

Loss of HuR Is Linked to Reduced Expression of Proliferative Genes during Replicative Senescence

Contact Info

Product

Resources

About