Collagenase-I, stromelysin-I, and matrilysin are expressed within theplacenta during multiple stages of human pregnancy

Vettraino, Ivana M.; Roby, Jill D.; Tolley, Timothy; Parks, William C.

doi:10.1016/s0143-4004(96)80072-5

Cited by 86 publications

(51 citation statements)

References 22 publications

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“…[21][22][23][24][25] Similarly, Wnt3A-dependent induction of cyclin D1 could provoke elevated proliferation of cytotrophoblasts, whereas Wnt-inducible genes promoting trophoblast invasion have yet to be elucidated. However, MMP-7 and MT1-MMP are expressed in invasive, extravillous trophoblasts, 75,76 supporting the idea that these enzymes could also be Wnt-targets in the human placenta.…”

Section: Discussionmentioning

confidence: 79%

Activation of the Canonical Wingless/T-Cell Factor Signaling Pathway Promotes Invasive Differentiation of Human Trophoblast

Pollheimer

Loregger

Sonderegger

et al. 2006

The American Journal of Pathology

169

192

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The molecular mechanisms governing invasive differentiation of human trophoblasts remain largely elusive. Here, we investigated the role of Wnt-␤-catenin-T-cell factor (TCF) signaling in this process. Reverse transcriptase-polymerase chain reaction and Western blot analyses demonstrated expression of Wnt ligands, frizzled receptors, LRP-6, and TCF-3/4 transcription factors in total placenta and different trophoblast cell models. Immunohistochemistry of placental tissues and differentiating villous explant cultures showed that expression of TCF-3/4 strongly increased in invading trophoblasts. Some of these cells also accumulated dephosphorylated ␤-catenin in the nucleus. Wnt3A treatment of primary cytotrophoblasts and SGHPL-5 cells induced activity of TCF-luciferase reporters. Accordingly, the ligand provoked interaction of TCF-3/4 with ␤-catenin as assessed in electrophoretic mobility shift assays (EMSAs) and upregulation of Wnt/TCF target genes as observed by Western blot analyses. Wnt3A stimulated trophoblast migration and invasion through Matrigel, which could be blocked by addition of Dickkopf-1, mediating inhibition of canonical Wnt signaling. Dickkopf-1 also reduced basal migration, invasion, and proliferation of cytotrophoblasts, suggesting expression of endogenous Wnt ligand(s). Immunohistochemistry revealed that the percentage of extravillous trophoblasts containing nuclear ␤-catenin was significantly higher in placentas of complete hydatidiform mole pregnancies as compared to normal placentas. Thus, canonical Wnt signaling may promote invasive trophoblast differentiation, and exaggerated activation of the pathway could contribute to trophoblastic hyperplasia and local invasion.

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Section: Discussionmentioning

confidence: 79%

Activation of the Canonical Wingless/T-Cell Factor Signaling Pathway Promotes Invasive Differentiation of Human Trophoblast

Pollheimer

Loregger

Sonderegger

et al. 2006

The American Journal of Pathology

169

192

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“…Nevertheless, the possibility that Mcol-A and Mcol-B are involved in other processes distinct from blastocyst invasion, including angiogenesis regulation or collagen turnover accompanying decidualization, cannot be ruled out. It is also noteworthy in the context of the putative relationships between these novel murine MMPs and human MMP-1, that this interstitial collagenase has been also found to be produced by trophoblastic cells during human pregnancy (43,44). These data suggest that the parallelisms between human MMP-1, and murine Mcol-A and Mcol-B, could be extended to their respective expression patterns.…”

Section: Analysis Of Mcol-a and Mcol-b Expression During Murine Embrymentioning

confidence: 82%

Yeast Nuclear Extract Contains Two Major Forms of RNA Polymerase II Mediator Complexes

Liu

Ranish

Aebersold

et al. 2001

Journal of Biological Chemistry

178

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Remodeling of fibrillar collagen in mouse tissues has been widely attributed to the activity of collagenase-3 (matrix metalloproteinase-13 (MMP-13)), the main collagenase identified in this species. This proposal has been largely based on the repeatedly unproductive attempts to detect the presence in murine tissues of interstitial collagenase (MMP-1), a major collagenase in many species, including humans. In this work, we have performed an extensive screening of murine genomic and cDNA libraries using as probe the full-length cDNA for human MMP-1. We report the identification of two novel mem-

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“…The fetal trophoblast is one likely candidate, suggested by our recent observation that trophoblasts in PIH have enhanced FasL expression (24). FasL is an integral membrane protein that can be cleaved into its soluble form by the MMP matrilysin (32), which is overexpressed by trophoblasts in preeclamptic placentas (33). Continuous exposure of the fetal trophoblast to maternal blood would allow FasL, cleaved from the trophoblast into soluble form, access to both the maternal and fetal circulations, and is consistent with the similar maternal and CB concentrations of sFasL that we showed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Fas and Fas Ligand Expression in Maternal Blood and in Umbilical Cord Blood in Preeclampsia

et al. 2001

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The Fas-Fas ligand (FasL) pathway of apoptosis is abnormally activated in diseases associated with impaired immune tolerance or chronic inflammation. Pregnancy-related hypertension is a spectrum of disease that commonly causes significant morbidity in women and in their newborn infants, is associated with generalized inflammation, and may be causally related to impaired maternal-fetal tolerance. Our recent observation of enhanced trophoblast expression of FasL in one form of pregnancy-related hypertension led us to hypothesize that this group of disorders might be associated with abnormal activation of the Fas-FasL pathway. To test this hypothesis, we prospectively quantified soluble and leukocyte-associated Fas receptor and FasL in the maternal and umbilical cord blood (CB) sera of 20 gestations complicated by preeclampsia and of 18 normal control gestations, using ELISA and flow cytometric analyses. We determined higher soluble FasL levels in paired maternal and CB sera of hypertensive gestations compared with control gestations (p Ͻ 0.01); in contrast, soluble Fas levels were similar between groups. Surface expression of FasL was lower on maternal (p Ͻ 0.01) and CB (p Ͻ 0.05) neutrophils from affected gestations, whereas surface Fas expression was lower on maternal (p Ͻ 0.02), but not CB, neutrophils and lymphocytes. We conclude that expression of Fas and FasL in sera and on leukocytes is altered in gestations complicated by preeclampsia, and speculate that activation of the Fas-FasL pathway mediates associated pathologic processes in affected women and in their neonates. Activation of the Fas-Fas Ligand pathway involves cellular interactions between the Fas receptor and FasL, and can result in apoptosis or in an inflammatory response (1). The Fas receptor (CD95, Apo-1, FasR), a type I integral membrane protein belonging to the tumor necrosis factor receptor family, is expressed on numerous cell types including hematopoietic cells, and its expression increases during inflammation (1). In contrast, expression of FasL (CD95L), a type II transmembrane molecule belonging to the tumor necrosis factor receptor superfamily, is limited to certain leukocytes and tissues with immune privilege (2). Fas-FasL interactions leading to clonal deletion of antigen-presenting cells have been implicated in the establishment of graft tolerance (3), and provide one explanation for the immune privilege status of certain tissues, including the placenta, cornea, and testis (2). In contrast, abnormal function of the Fas-FasL pathway is involved in the pathogenesis of a variety of disorders, including cytopenias, autoimmune diseases, graft-versus-host disease, and graft rejection (1,(3)(4)(5)(6).Mice with the gld mutation are deficient in FasL function and have smaller litters with decreased viability (7). Studies in these mice suggest that the Fas-FasL pathway contributes to preservation of the fetal allograft by preventing the trafficking of activated leukocytes between the mother and fetus during normal gestation, observations also ...

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Collagenase-I, stromelysin-I, and matrilysin are expressed within theplacenta during multiple stages of human pregnancy

Cited by 86 publications

References 22 publications

Activation of the Canonical Wingless/T-Cell Factor Signaling Pathway Promotes Invasive Differentiation of Human Trophoblast

Activation of the Canonical Wingless/T-Cell Factor Signaling Pathway Promotes Invasive Differentiation of Human Trophoblast

Yeast Nuclear Extract Contains Two Major Forms of RNA Polymerase II Mediator Complexes

Fas and Fas Ligand Expression in Maternal Blood and in Umbilical Cord Blood in Preeclampsia

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