Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications

Brito, Adrianne M. M.; Oliveira, Vitor; Icimoto, Marcelo Yudi; Nantes, Iseli L.

doi:10.3390/pharmaceutics13081143

“…Brito et al. ( 20 ) showed that stability and gain of specific activity of bromelain protein complexes can be improved by immobilizing bromelain on gold nanoparticles. By applying CD spectroscopy, the authors observed structural changes in proteins upon binding to nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

BeStSel: webserver for secondary structure and fold prediction for protein CD spectroscopy

Micsonai

¹

,

Moussong

²

,

Wien

³

et al. 2022

Nucleic Acids Research

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Circular dichroism (CD) spectroscopy is widely used to characterize the secondary structure composition of proteins. To derive accurate and detailed structural information from the CD spectra, we have developed the Beta Structure Selection (BeStSel) method (PNAS, 112, E3095), which can handle the spectral diversity of β-structured proteins. The BeStSel webserver provides this method with useful accessories to the community with the main goal to analyze single or multiple protein CD spectra. Uniquely, BeStSel provides information on eight secondary structure components including parallel β-structure and antiparallel β-sheets with three different groups of twist. It overperforms any available method in accuracy and information content, moreover, it is capable of predicting the protein fold down to the topology/homology level of the CATH classification. A new module of the webserver helps to distinguish intrinsically disordered proteins by their CD spectrum. Secondary structure calculation for uploaded PDB files will help the experimental verification of protein MD and in silico modelling using CD spectroscopy. The server also calculates extinction coefficients from the primary sequence for CD users to determine the accurate protein concentrations which is a prerequisite for reliable secondary structure determination. The BeStSel server can be freely accessed at https://bestsel.elte.hu.

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“…Finally, a study on the collagenase activity found in bromelain was conducted; here, the enzyme was immobilized with the use of a gold nanoparticle interface, which resulted in improvements in the thermal stability and enzymatic activity of this biomaterial in therapeutic applications that rely on the anti-inflammatory, anticoagulant, antitumor, antimetastatic in vitro properties and in vivo immunogenicity properties [98].…”

Section: Enzymatic Productsmentioning

confidence: 99%

Pineapple Agro-Industrial Wastes to Produce Biomedical Applications Post-COVID-19 Pandemic: Biorefinery and the Circular Economy

Amores-Monge¹,

Goyanes²,

Ribba³

et al. 2022

Preprint

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Pineapple is a highly demanded fruit in international markets, thanks to its unique appearance and flavor, high fiber content, vitamins, folic acid, and minerals. It makes the pineapple production and processing market a significant source of income for producing countries, such as Costa Rica. Nowadays, its processing produces a large amount of waste with negative consequences for the environment. However, pineapple waste is an essential source of cellulose, hemicellulose, lignin, and other high-value products like enzymes (bromelain). These by-products can be obtained by pineapple waste biorefinery, generating an additional source of export goods and foreign currency, framing pineapple processing in the concept of the circular economy. This review discusses how incorporating biorefinery in the pineapple production processes can contribute to the post-COVID 19 economy in Costa Rica. Pineapple production in Costa Rica is explored, and the contamination of generated residues is delineated. Furthermore, the primary processes for by-product extraction via biorefinery, their general characteristics and applications in the medical field, and their contribution to the circular economy are presented.

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“…Finally, a study on the collagenase activity found in bromelain was conducted; here, the enzyme was immobilized with the use of a gold nanoparticle interface, which resulted in improvements in the thermal stability and enzymatic activity of this biomaterial in therapeutic applications that rely on the anti-inflammatory, anticoagulant, antitumor, antimetastatic in vitro properties and in vivo immunogenicity properties [97].…”

Section: Enzymatic Productsmentioning

confidence: 99%

Pineapple Agro-Industrial Biomass to Produce Biomedical Applications in a Circular Economy Context in Costa Rica

Amores-Monge¹,

Goyanes²,

Ribba³

et al. 2022

Preprint

2

0

View full text Add to dashboard Cite

Pineapple is a highly demanded fruit in international markets, thanks to its unique appearance and flavor, high fiber content, vitamins, folic acid, and minerals. It makes the pineapple production and processing market a significant source of income for producing countries, such as Costa Rica. Nowadays, its processing produces a large amount of waste with negative consequences for the environment. However, pineapple waste is an essential source of cellulose, hemicellulose, lignin, and other high-value products like enzymes (bromelain). These by-products can be obtained by pineapple waste biorefinery, generating an additional source of export goods and foreign currency, framing pineapple processing in the concept of the circular economy. This review discusses how incorporating biorefinery in the pineapple production processes can contribute to the post-COVID 19 economy in Costa Rica. Pineapple production in Costa Rica is explored, and the contamination of generated residues is delineated. Furthermore, the primary processes for by-product extraction via biorefinery, their general characteristics and applications in the medical field, and their contribution to the circular economy are presented.

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Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications

Cited by 16 publications

References 41 publications

BeStSel: webserver for secondary structure and fold prediction for protein CD spectroscopy

BeStSel: webserver for secondary structure and fold prediction for protein CD spectroscopy

Pineapple Agro-Industrial Wastes to Produce Biomedical Applications Post-COVID-19 Pandemic: Biorefinery and the Circular Economy

Pineapple Agro-Industrial Biomass to Produce Biomedical Applications in a Circular Economy Context in Costa Rica

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