Collagenase activities in healthy and inflamed gingiva of dogs

Abstract

The effects of inflammation on collagenase and gelatinase were studied in dog gingiva. Inflammation was induced by the placement of cervical ligatures. Collagenase activity was measured using soluble [14C]‐acetylated collagen with high specific activity as substrate and separating the reaction products by 50% dioxane (Terato et al. 1976). Two forms of collagenase, a soluble enzyme free from the substrate and an insoluble enzyme bound to the substrate, were extracted by neutral salt solution and by sequential sonication in low and high salt buffers, respectively. Treatment with a high concentration of trypsin was necessary for detection of collagenase activities in both extracts. The collagenase activities in the neutral salt extract and the first sonic extract were significantly higher in inflamed gingiva after 1 week of plaque formation than in healthy gingiva, but that in the second sonic extract showed no significant increase. After 3 weeks of plaque formation, the activities in neutral salt extract and the first sonic extract of inflamed gingiva similarly showed significant increases relative to controls. On the other hand, gelatinase activity did not vary significantly with the induction of inflammation.

The effect of oxygen‐derived free radicals on gingival proteoglycans and hyaluronic acid

“…The data reported herein are particularly interesting in light of the current concepts of the pathogenesis of periodontal disease (Bartold, Wiebkin & Thonard 1983c). At present, enzymes such as hyaluronidase (Gaffer, Coleman & Marcussen 1981), collagenase (Uitto, Appelgren & Robinson 1981, WooUey & Davies 1981, Yanagimura, Hara & Nohara 1983, and proteases released by leukocytes (Cergneaux, Anderson & Cimansoni 1982) are considered to be responsible for much of the disruption to the gingival connective tissues affected by periodontal disease. However, whilst these enzymes can undoubtedly degrade hyaiuronic acid, proteoglycans, and collagen in vitro, proponents of this philosophy have had difficulty in isolating these enzymes from gingivae as well as difficulties in demonstrating in vivo enzymatic activity.…”

Demonstration of tissue collagenase activity in vivo and its relationship to inflammation severity in human gingiva

“…Tissue collagenase has also been demonstrated in gingival crevicular fluid (Golub et al 1976, Uitto & Raeste 1978, Kryshtalskyj, Sodek & Ferrier 1986, Larivee, Sodek & Ferrier 1986, directly shown in inflamed gingiva by immunohistochemical-localization techniques (WooUey & Davies 1981) and extracted, with difflculty, from pooled gingival tissue homogenates (Uitto, Turto & Saxen 1978, Uitto, Appelgren & Robinson 1981, Yanagimura, Hara & Nohara 1983. However, direct evidence for in vivo collagenase activity in the periodontium during the course of the inflammatory process is lacking.…”

Factors Affecting IL-1-Mediated Collagen Metabolism By Fibroblasts and the Pathogenesis of Periodontal Disease: A Review of the Literature

“…Almost 10 years later, collagenolytic activity was extracted from homogenates of pooled human gingiva from subjects with periodontal disease (Uitto et al, 1978). The presence of collagenase activity in gingiva was also confirmed in experimental gingivitis in dogs (Yanagimura et al, 1983). Direct isolation of 3/4 cleavage fragments from tissue extracts has been used to implicate the activity of genuine collagenases in tissue destruction in inflamed gingiva in vivo (Overall et al, 1987).…”