Collagenase-1 (-1607 1G/2G), Gelatinase-A (-1306 C/T), Stromelysin-1 (-1171 5A/6A) functional promoter polymorphisms in risk prediction of type 2 diabetic nephropathy

Gantala, Srilatha Reddy; Kondapalli, Mrudula Spurthi; Kummari, Ramanjaneyulu; Padala, Chiranjeevi; Tupurani, Mohini Aiyengar; Kupsal, Keerthi; Galimudi, Rajesh Kumar; Gundapaneni, Kishore Kumar; Puranam, Kaushik; Shyamala, Nivas; Swarnalatha, G; Ram, R; Hanumanth, Surekha Rani

doi:10.1016/j.gene.2018.06.007

Cited by 13 publications

(18 citation statements)

References 40 publications

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“…DN development has been associated with various causes, including genetic predisposition, hyperglycemia, albuminuria, glomerular sclerosis and hemodynamic alterations in the kidney (Kaifu et al., 2018). A recent statistical report has revealed that patients with 15‐year DN history have a death rate of 30% to 40% (Gantala et al., 2018). Therefore, managing this fatal complication of DM is of utmost importance.…”

Section: Introductionmentioning

confidence: 99%

Cassia auriculata leaf extract ameliorates diabetic nephropathy by attenuating autophagic necroptosis via RIP‐1/RIP‐3‐p‐p38MAPK signaling

et al. 2021

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Diabetic nephropathy (DN) is the most common manifestation of high glucose induced diabetes mellitus. In this study, we report the effects of Cassia auriculata ethanol leaf extract (CALE) on DN‐associated cell toxicity and complications. The effects of CALE were screened in vitro using RGE cells. Cell viability was assessed using MTT and flow cytometry. Male Sprague‐Dawley rats were divided into control, DN and treatment groups (n = 8). The DN and treatment groups received 60 mg/kg/bw of streptozotocin in citrate buffer, while the treatment group was administered 150 mg/kg/bw of CALE for 10 weeks. Biochemical analysis was conducted using spectrophotometry. Kidney tissues were analyzed using hematoxylin and eosin staining and transmission electron microscopy. CD365‐KIM‐1 expression was assessed using flow cytometry and signalling proteins were detected using western blotting. Treatment with 30‐mM glucose reduced the viability of RGE cells in a time‐dependent manner and increased the population of dead RGE cells. Cotreatment with CALE reduced cell death and glucose induced protein expression of LC3‐II, RIP‐1 and RIP‐3 in a dose‐dependent manner. In addition, CALE improved the biochemical complications, renal dysfunction and pathophysiology of rats with DN and partially or fully restored the expression of key DN‐associated signalling proteins, such as KIM‐1 LC3‐II, RIP‐1, RIP‐3 and p‐p38MAPK in kidney cells. CALE showed protective effects, and improved DN‐associated complications in RGE cells under high glucose stress conditions, potentially by inhibiting autophagic‐necroptosis signals. Additionally, CALE improved the biochemical and pathological features of kidney injury while reducing autophagic‐necroptosis in rat renal cells via the LC3‐II‐RIP‐p38MAPK pathway. Practical applications Results from the current investigation will add information to the literature on glucose induced renal toxicity and the protective effects of CALE over the complications of diabetic nephropathy (DN). The mechanistic investigations of the study will add light on the autophagic/necroptosis signals in DN and open new routes of investigations to study the efficacy of CALE in diabetes‐related complications.

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Section: Introductionmentioning

confidence: 99%

Cassia auriculata leaf extract ameliorates diabetic nephropathy by attenuating autophagic necroptosis via RIP‐1/RIP‐3‐p‐p38MAPK signaling

et al. 2021

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“…Lastly, we predicted transcription factors located near 10 topranked DMPs through in silico analysis. The DNA sequences around the methylation locations were obtained using ENSENBL, and the promotor binding sites located near the methylated points were determined using Alibaba 2.1 [28]. 1).…”

Section: Discussionmentioning

confidence: 99%

Differential DNA methylation following chemotherapy for breast cancer is associated with lack of memory improvement at one year

Yang

Jackson‐Cook

et al. 2019

Epigenetics

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The biological basis underlying cognitive dysfunction in women with early-stage breast cancer (BC) remains unclear, but could reflect gene expression changes that arise from the acquisition and long-term retention of soma-wide alterations in DNA methylation in response to chemotherapy. In this longitudinal study, we identified differences in peripheral methylation patterns present in women prior to treatment (T1) and 1 year after receiving chemotherapy (T4) and evaluated relationships among the differential methylation (DM) ratios with changes in cognitive function. A total of 58 paired (T1 and T4) blood specimens were evaluated. Methylation values were determined for DNA isolated from whole blood using a genome-wide array . Cognitive function was measured using the validated, computerized CNS Vital Signs platform. Relationships between methylation patterns and cognitive domain scores were compared using a stepwise linear regression analysis, with demographic variables as covariates. The symptom comparison analysis was restricted to 2,199 CpG positions showing significant methylation ratio changes between T1 and T4. The positions with DM were enriched for genes involved in the modulation of cytokine concentrations. Significant DM ratios were associated with memory domain (56 CpGs). Eight of the ten largest DM ratio changes associated with lack of memory improvement were localized to genes involved in either neural function (ECE2, PPFIBP2) or signalling processes (USP6NL, RIPOR2, KLF5, UBE2V1, DGKA, RPS6KA1). These results suggest that epigenetic changes acquired and retained for at least one year in non-tumour cells following chemotherapy may be associated with a lack of memory improvement following treatment in BC survivors.

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“…Some show that certain polymorphisms of MMP-9 may be predictive factors in the development of diabetic nephropathy [30] and some suggest the protective role of the T allele SNP -1562C>T of MMP-9 against diabetic nephropathy [31,32]. It has also been shown that MMP-2 gene polymorphism is associated with susceptibility and disease progression of DN [33].…”

Section: Introductionmentioning

confidence: 99%

Matrix metalloproteinase-2 (MMP-2 ) and-9 (MMP-9) gene variants and microvascular complications in type 2 diabetes patients

Andjelic

Radojković

Nikolić

et al. 2022

Balkan Journal of Medical Genetics

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Vascular complications are the leading cause of increased morbidity and mortality of diabetic patients. It has been postulated that matrix metalloproteinases MMP-2 and MMP-9, zinc-dependent endopeptidases through remodeling of the extracellular matrix, can contribute to the onset and progression of diabetic vascular complications. The aim of our study was to assess whether there is a major difference in single nucleotide polymorphisms in the MMP-2 (at position -1306C˃T) and MMP-9 (at position -1562C˃T) gene in type 2 diabetic patients and healthy controls and to determine whether there is an association of these gene variants with the presence of microvascular complications in diabetic patients. Our study included 102 type 2 diabetes patients and a control group which was comprised of 56 healthy controls. All diabetic patients were screened for microvascular diabetes complications. Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies were determined. The MMP-2 variant -1306C>T showed a negative correlation with type 2 diabetes (p=0.028). It was also shown that the presence of the -1306C allele increases the probability of developing type 2 diabetes. This was a 2.2 fold increase and that the -1306 T allele has a protective role in regards to type 2 diabetes. The MMP-2 variant -1306T showed a negative correlation with diabetic polyneuropathy (p=0.017), meaning that allele-1306T has a protective role in regards to diabetic polyneuropathy while the presence of allele -1306C increases the probability of developing diabetic polyneuropathy by 3.4 fold. Our study showed that the MMP-2 gene variant (-1306C) doubles the risk of developing type 2 diabetes, and for the first time an association of this gene variant and the presence of diabetic polyneuropathy was shown.

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Collagenase-1 (-1607 1G/2G), Gelatinase-A (-1306 C/T), Stromelysin-1 (-1171 5A/6A) functional promoter polymorphisms in risk prediction of type 2 diabetic nephropathy

Cited by 13 publications

References 40 publications

Cassia auriculata leaf extract ameliorates diabetic nephropathy by attenuating autophagic necroptosis via RIP‐1/RIP‐3‐p‐p38MAPK signaling

Cassia auriculata leaf extract ameliorates diabetic nephropathy by attenuating autophagic necroptosis via RIP‐1/RIP‐3‐p‐p38MAPK signaling

Differential DNA methylation following chemotherapy for breast cancer is associated with lack of memory improvement at one year

Matrix metalloproteinase-2 (MMP-2 ) and-9 (MMP-9) gene variants and microvascular complications in type 2 diabetes patients

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