Collagen type V modulates fibroblast behavior dependent on substrate stiffness

Breuls, Roel G.M.; Klumpers, Darinka D.; Everts, Vincent; Smit, Theo H.

doi:10.1016/j.bbrc.2009.01.110

Cited by 38 publications

(34 citation statements)

References 26 publications

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“…Here we have focused on differences in extracellular matrix and adhesion protein expression, and show that types V and XII collagens as well as integrin subunits alpha 3 and alpha 6 and thrombospondin-1 are upregulated in RDEBF and UVSCCF fibroblasts and further upregulated in RDEBSCCF fibroblasts. Types V and XII collagens are reported to have roles in cellular adhesion and migration (30,31) and the increased fibroblast-mediated contraction of type V collagen-containing gels is integrin dependent (32) in agreement with integrin a3-dependent increased SCC invasion into fibroblast remodeled gels (16 (34,35), although more recently type XII collagen has been reported to be highly expressed by cancer-associated fibroblasts in colon cancer (36), suggesting that cancer-associated type XII collagen expression is context dependent. Thrombospondin upregulation in the stroma of esophageal adenocarcinoma identifies patients with poor prognosis (37) and a recent gene expression study correlating stromal activation with metastasis in head and neck SCC identified COL5A1 and TSP1 as the top 2 differentially expressed genes (38) supporting their clinical relevance in other aggressive cancers.…”

Section: Discussionmentioning

confidence: 67%

Fibroblast-Derived Dermal Matrix Drives Development of Aggressive Cutaneous Squamous Cell Carcinoma in Patients with Recessive Dystrophic Epidermolysis Bullosa

et al. 2012

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Patients with the genetic skin blistering disease recessive dystrophic epidermolysis bullosa (RDEB) develop aggressive cutaneous squamous cell carcinoma (cSCC). Metastasis leading to mortality is greater in RDEB than in other patient groups with cSCC. Here we investigate the dermal component in RDEB using mRNA expression profiling to compare cultured fibroblasts isolated from individuals without cSCC and directly from tumor matrix in RDEB and non-RDEB samples. Although gene expression of RDEB normal skin fibroblasts resembled that of cancer-associated fibroblasts, RDEB cancer-associated fibroblasts exhibited a distinct and divergent gene expression profile, with a large proportion of the differentially expressed genes involved in matrix and cell adhesion. RDEB cancer-associated fibroblasts conferred increased adhesion and invasion to tumor and nontumor keratinocytes. Reduction of COL7A1, the defective gene in RDEB, in normal dermal fibroblasts led to increased type XII collagen, thrombospondin-1, and Wnt-5A, while reexpression of wild type COL7A1 in RDEB fibroblasts decreased type XII collagen, thrombospondin-1, and Wnt-5A expression, reduced tumor cell invasion in organotypic culture, and restricted tumor growth in vivo. Overall, our findings show that matrix composition in patients with RDEB is a permissive environment for tumor development, and type VII collagen directly regulates the composition of matrix proteins secreted by dermal and cancer-associated fibroblasts.

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Section: Discussionmentioning

confidence: 67%

Fibroblast-Derived Dermal Matrix Drives Development of Aggressive Cutaneous Squamous Cell Carcinoma in Patients with Recessive Dystrophic Epidermolysis Bullosa

et al. 2012

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“…Tumor progression in particular is characterized by increased ECM deposition, termed "desmoplasia." Moreover, collagen V is increased in desmoplastic stroma (Barsky et al 1982), which is mechanically significant as collagen V changes the structure of collagen I fibrils (Wenstrup et al 2004;Berendsen et al 2006;Breuls et al 2009). In addition, several other proteins such as fibronectin and proteoglycans bind to collagens and affect the organization of collagen fibers, and thereby have effects on the mechanical milieu.…”

mentioning

confidence: 99%

Mammary Gland ECM Remodeling, Stiffness, and Mechanosignaling in Normal Development and Tumor Progression

Schedin¹,

Keely²

2010

Cold Spring Harbor Perspectives in Biology

365

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“…High elevation of production of collagen type III as compared to collagen type I in phases of early ligament healing is detected. Liu et al [19] and Breuls et al [20] state that collagen type V is associated with collagen type I and regulates the collagen fibril diameter. The enhanced levels of type V collagen influence the stiffness of the extracelular matrix by changing the fibril organization of extracellular matrix [21].…”

Section: Types Of Collagens In the Elmentioning

confidence: 99%

The Epiligament-The Main Donor of Cells and Vessels during Healing Of the Collateral Ligaments of the Knee

Landzhov¹

2015

Anat Physiol

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Ligaments are composed of dense connective tissue and attach bones in joints. The thin connective tissue sheath, covering these fascicles is called endoligament and is connected to a more vascular connective tissue structure that envelopes the entire ligament and is referred to as epiligament. The tissue of the epiligaments is composed of different cell types such as: fibroblasts, fibrocytes, adipocytes, neurovascular bundles, and a multitude of collagen fibers, disposed in different directions. The main structural protein of epiligament is collagen type I. Collagens types III and V were also found in the structure of epiligament. Type I collagen is the main collagen in normal and healing ligaments. The ligament repair requires presence of collagen type III. Collagen type V is associated with collagen type I and regulates the collagen fibril diameter. Knowledge of variation of cells and collagen types of epiligament in normal and injured ligaments is crucial for understanding of the healing process. Keywords: Collateral ligament epiligament; Knee Abbreviations:EL: Epiligament; MCL: Medial Collateral Ligament Ligament and Epiligament (EL) StructureThe structures, composed of dense connective tissues that attach bones in joints are known as ligaments. The main components of ligaments connective tissue are ligament cells and extracellular matrix [1,2]. The internal gross organization of ligaments includes fascicles which are composed of collagen fibers arranged in longitudinal groups [3]. The thin connective tissue sheath, covering these fascicles is called endoligament and is connected to a more vascular connective tissue structure that envelopes the entire ligament and is referred to as epiligament (EL) [4].The first definition of the term in the scientific literature is given by Bray et al. [5], described it as "surrounding adherent connective tissue removed simultaneously with the ligament but which was grossly distinguishable from ligament tissue proper". In the external area of medial collateral ligament (MCL) in rabbits only two types of cells were observed -spinous and cuboidal shaped fibroblasts and fat cells [4]. In addition Georgiev et al. [6,7] described different types of fibroblasts -spinous-shaped, spindle-shaped, elongated, irregular in shape and fat cells.There are relatively few data about the structural and physiological features of the EL, therefore herein we review the existing data about this structure in the literature and lay emphasis on its clinical significance. Macroscopic AppearanceThe EL is a thin translucent layer which entirely covers the ligament and in the same time mobile in all directions. Its compliance is higher than that of the underlying ligament. At the ligament insertion sites the EL is continuous with the periosteum. It is attached to the MCL by fronds of synovium. In the deeper areas which are towards the joint cavity, the EL is attached tighter to the ligament than in the superficial ones. The EL is more difficult to distinguish from MCL in immature animals. There...

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Collagen type V modulates fibroblast behavior dependent on substrate stiffness

Cited by 38 publications

References 26 publications

Fibroblast-Derived Dermal Matrix Drives Development of Aggressive Cutaneous Squamous Cell Carcinoma in Patients with Recessive Dystrophic Epidermolysis Bullosa

Fibroblast-Derived Dermal Matrix Drives Development of Aggressive Cutaneous Squamous Cell Carcinoma in Patients with Recessive Dystrophic Epidermolysis Bullosa

Mammary Gland ECM Remodeling, Stiffness, and Mechanosignaling in Normal Development and Tumor Progression

The Epiligament-The Main Donor of Cells and Vessels during Healing Of the Collateral Ligaments of the Knee

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