Collagen synthesis is required for ascorbic acid-enhanced differentiation of mouse embryonic stem cells into cardiomyocytes

Sato, Hajime; Takahashi, Masafumi; Ise, Hirohiko; Yamada, Ai; Hirose, Seiyu; Tagawa, Yoh‐ichi; Morimoto, Hajime; Izawa, Atsushi; Ikeda, Uichi

doi:10.1016/j.bbrc.2006.01.116

Cited by 87 publications

(72 citation statements)

References 22 publications

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“…Media changes were performed every day. Differentiation of ES cells to cardiomyocites 36,37 and neurons 38 was performed following published protocols. Non-lineage specific differentiation was performed by removal of the feeder layer and LIF from the culture media.…”

Section: Methodsmentioning

confidence: 99%

MDM4 downregulates p53 transcriptional activity and response to stress during differentiation

Menéndez

Goh²,

Camus³

et al. 2011

Cell Cycle

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Section: Methodsmentioning

confidence: 99%

MDM4 downregulates p53 transcriptional activity and response to stress during differentiation

Menéndez

Goh²,

Camus³

et al. 2011

Cell Cycle

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“…121 Many cell types such as fibroblasts, mesenchymal stem cells, epithelial cells and neural crest cells show different adhesions when grown on 3D matrices as opposed to 2D cell culture. [122][123][124] A possible reason is that the 3D architecture could distribute binding sites in a variety of special locations rather than on only the single plane of rigid substrate as in traditional 2D architecture. 124,125 Cells may have cytoskeletal adaptor proteins on 3D matrix in addition to proteins present in 2D focal adhesions.…”

Section: Cellular Interactionmentioning

confidence: 99%

“…[122][123][124] A possible reason is that the 3D architecture could distribute binding sites in a variety of special locations rather than on only the single plane of rigid substrate as in traditional 2D architecture. 124,125 Cells may have cytoskeletal adaptor proteins on 3D matrix in addition to proteins present in 2D focal adhesions. 124,126 For example, focal adhesion kinase (FAK) in 3D matrix adhesion is poorly phosphorylated at its major tyrosine phosphorylation site for cell adhesion.…”

Section: Cellular Interactionmentioning

confidence: 99%

“…124,125 Cells may have cytoskeletal adaptor proteins on 3D matrix in addition to proteins present in 2D focal adhesions. 124,126 For example, focal adhesion kinase (FAK) in 3D matrix adhesion is poorly phosphorylated at its major tyrosine phosphorylation site for cell adhesion. Such differences in cell adhesion between 2D and 3D matrices lead to different signal transduction and subsequent alteration in cellular rearrangement.…”

Section: Cellular Interactionmentioning

confidence: 99%

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Cell colonization in degradable 3D porous matrices

Lawrence

Madihally

2008

Cell Adhesion & Migration

174

155

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Cell colonization is an important in a wide variety of biological processes and applications including vascularization, wound healing, tissue engineering, stem cell differentiation and biosensors. During colonization porous 3D structures are used to support and guide the ingrowth of cells into the matrix. In this review, we summarize our understanding of various factors affecting cell colonization in three-dimensional environment. The structural, biological and degradation properties of the matrix all play key roles during colonization. Further, specific scaffold properties such as porosity, pore size, fiber thickness, topography and scaffold stiffness as well as important cell material interactions such as cell adhesion and mechanotransduction also influence colonization.

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“…Many cell types such as fibroblasts and mesenchymal, epithelial, and neural crest cells attached to 3D matrices show distinct adhesions from 2D culture. 165,174 A possible consensus is that the 3D architecture could distribute binding sites differently from 2D architecture. 174,175 3D focal adhesions appear distinct from 2D focal adhesions on a rigid 2D matrix and were termed as ''3D matrix adhesions'' to separate them from 2D counterparts.…”

Section: Performing Cell Cultures In 3dmentioning

confidence: 99%

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Hong

Madihally

2011

Tissue Engineering Part B: Reviews

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Electrospinning is a widely established polymer-processing technology that allows generation of fibers (in nanometer to micrometer size) that can be collected to form nonwoven structures. By choosing suitable process parameters and appropriate solvent systems, fiber size can be controlled. Since the technology allows the possibility of tailoring the mechanical properties and biological properties, there has been a significant effort to adapt the technology in tissue regeneration and drug delivery. This review focuses on recent developments in adapting this technology for tissue regeneration applications. In particular, different configurations of nozzles and collector plates are summarized from the view of cell seeding and distribution. Further developments in obtaining thick layers of tissues and thin layered membranes are discussed. Recent advances in porous structure spatial architecture parameters such as pore size, fiber size, fiber stiffness, and matrix turnover are summarized. In addition, possibility of developing simple three-dimensional models using electrosprayed fibers that can be utilized in routine cell culture studies is described. Tissue EngineeringT issue engineering or regeneration is a multidisciplinary study to restore, maintain, and enhance tissue and organ function. 1 In tissue engineering, biodegradable scaffolds are used to support and guide cells to proliferate, organize, and produce their own extracellular matrix (ECM). Scaffolding material eventually disappears leaving only the necessary healthy tissue in a topologically required form. 2,3 Assembly and maturation of ECM elements is important in determining the biomechanics and the quality of the tissue. For example, collagen provides tensile strength to the tissue, elastic fibers contribute to the elasticity of the tissue, while proteoglycans fills the extracellular space, creating a space for the tissue regulation of growth factors and other interactions. 4 Delicate balance between different matrix elements is necessary to generate healthy tissue. The size and shape of collagen fibers in ECM relies on tissues and organs even in the same species. The shape is cord or tape with a width of 1-20 mm and the collagen fibrils (unit can be observed by electron microscopy) are cylindrical with a diameter ranging from 10 to over 500 nm where cell is attaching and hugging. 5 Obtaining a biodegradable matrix conducive for cell colonization is a fundamental requirement for tissue regeneration. Like ECM, biomimic scaffold should allow cell attachment and migration, enables diffusion of vital cell nutrients, and retains cells. Further, chemical and mechanical properties of scaffold influence cell viability and proliferation. 6 Appropriate pore size and porosity are essential to modulate cell seeding and diffusion. Biodegradability is essential because scaffolds are absorbed and distributed to the nearby tissue, which allows no surgical removal from body. The surface of scaffold is suitable for cell attachment and migration. The mechanical properties...

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Collagen synthesis is required for ascorbic acid-enhanced differentiation of mouse embryonic stem cells into cardiomyocytes

Cited by 87 publications

References 22 publications

MDM4 downregulates p53 transcriptional activity and response to stress during differentiation

MDM4 downregulates p53 transcriptional activity and response to stress during differentiation

Cell colonization in degradable 3D porous matrices

Next Generation of Electrosprayed Fibers for Tissue Regeneration

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