Collagen-Supplemented Incubation Rapidly Augments Mechanical Property of Fibroblast Cell Sheets

Zhu, Yuanjia; Thakore, Akshara D.; Farry, Justin M.; Jung, Jinsuh; Anilkumar, Shreya; Wang, Shuangfeng; Imbrie-Moore, Annabel M.; Park, Matthew H.; Tran, Nicholas A.; Woo, Y. Joseph

doi:10.1089/ten.tea.2020.0128

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Cell sheet engineering using UpCell dishes is a modern technique that allows for the rapid formation of cell monolayers without the use of ECM enzymatic digestion. It is hydrophilic when the temperature is reduced to allow cell separation without altering cell-cell junctions and ECM deposition [133]. SDF-1, VEGF, HGF, and TGF-were all produced by these cell sheets [134] and increased the activation of FLK1 and VEGFR-2.…”

Section: Co-culturementioning

confidence: 99%

Recent Advances in Bioengineering Bone Revascularization Based on Composite Materials Comprising Hydroxyapatite

Niu

Chen

2023

IJMS

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The natural healing process of bone is impaired in the presence of tumors, trauma, or inflammation, necessitating external assistance for bone regeneration. The limitations of autologous/allogeneic bone grafting are still being discovered as research progresses. Bone tissue engineering (BTE) is now a crucial component of treating bone injuries and actively works to promote vascularization, a crucial stage in bone repair. A biomaterial with hydroxyapatite (HA), which resembles the mineral makeup of invertebrate bones and teeth, has demonstrated high osteoconductivity, bioactivity, and biocompatibility. However, due to its brittleness and porosity, which restrict its application, scientists have been prompted to explore ways to improve its properties by mixing it with other materials, modifying its structural composition, improving fabrication techniques and growth factor loading, and co-cultivating bone regrowth cells to stimulate vascularization. This review scrutinizes the latest five-year research on HA composite studies aimed at amplifying vascularization in bone regeneration.

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Section: Co-culturementioning

confidence: 99%

Recent Advances in Bioengineering Bone Revascularization Based on Composite Materials Comprising Hydroxyapatite

Niu

Chen

2023

IJMS

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“…Collectively, these findings also have potential for clinical translation as vascular conduits, demonstrated by using tubularized cell sheets in a rat femoral artery interposition graft model ( von Bornstädt et al, 2018 ). Importantly, the mechanical properties and specifically the stiffness of cell sheets can be easily modified by titrating the collagen content during incubation ( Zhu et al, 2020 ).…”

Section: Tissue Engineered Solutionsmentioning

confidence: 99%

The Expanding Armamentarium of Innovative Bioengineered Strategies to Augment Cardiovascular Repair and Regeneration

Elde

Wang

Woo

2021

Front. Bioeng. Biotechnol.

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Cardiovascular disease remains the leading cause of death worldwide. While clinical trials of cell therapy have demonstrated largely neutral results, recent investigations into the mechanisms of natural myocardial regeneration have demonstrated promising new intersections between molecular, cellular, tissue, biomaterial, and biomechanical engineering solutions. New insight into the crucial role of inflammation in natural regenerative processes may explain why previous efforts have yielded only modest degrees of regeneration. Furthermore, the new understanding of the interdependent relationship of inflammation and myocardial regeneration have catalyzed the emergence of promising new areas of investigation at the intersection of many fields.

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Decellularized Scaffolds with Double‐Layer Aligned Microchannels Induce the Oriented Growth of Bladder Smooth Muscle Cells: Toward Urethral and Ureteral Reconstruction

Cheng,

Zhang,

Zhang

et al. 2023

Adv Healthcare Materials

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There is a great clinical need for regenerating urinary tissue. Native urethras and ureters have bidirectional aligned smooth muscle cells (SMCs) layers, which plays a pivotal role in micturition and transporting urine and inhibiting reflux. Thus far, urinary scaffolds have not been designed to induce the native‐mimicking aligned arrangement of SMCs. In this study, a tubular decellularized extracellular matrix (dECM) with an intact internal layer and bidirectional aligned microchannels in the tubular wall, which is realized by the subcutaneous implantation of a template, followed by the removal of the template, and decellularization, is engineered. The dense and intact internal layer effectively increases the leakage pressure of the tubular dECM scaffolds. Rat‐derived dECM scaffolds with three different sizes of microchannels are fabricated by tailoring the fiber diameter of the templates. The rat‐derived dECM scaffolds exhibiting microchannels of ≈65 µm show suitable mechanical properties, good ability to induce the bidirectional alignment and growth of human bladder SMCs, and elevated higher functional protein expression in vitro. These data indicate that rat‐derived tubular dECM scaffolds manifesting double‐layer aligned microchannels may be promising candidates to induce the native‐mimicking regeneration of SMCs in urethra and ureter reconstruction.

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Collagen-Supplemented Incubation Rapidly Augments Mechanical Property of Fibroblast Cell Sheets

Cited by 5 publications

References 28 publications

Recent Advances in Bioengineering Bone Revascularization Based on Composite Materials Comprising Hydroxyapatite

Recent Advances in Bioengineering Bone Revascularization Based on Composite Materials Comprising Hydroxyapatite

The Expanding Armamentarium of Innovative Bioengineered Strategies to Augment Cardiovascular Repair and Regeneration

Decellularized Scaffolds with Double‐Layer Aligned Microchannels Induce the Oriented Growth of Bladder Smooth Muscle Cells: Toward Urethral and Ureteral Reconstruction

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