Collagen structural organization of healing colonic anastomoses and the effect of growth hormone treatment

Abstract

Healing colonic anastomoses are characterized by new-formed collagen fibrils at postoperative day 4, and bGH seems to stimulate structural organization of the anastomotic collagen fibrils into fibers.

Does the Administration Route of Leucovorin Have Any Influence on the Impairment of Colonic Healing Caused by Intraperitoneal 5-Fluorouracil Treatment?

“…The colonic wall derives its strength mainly from collagen fibrils located in the submucosa [28]. After surgery early anastomotic strength depends on the suture-holding capacity because up to 4 days normal anastomosis was filled with loosely packed and unorganized collagen fibrils.…”

The Effect of Azathioprine on Anastomotic Healing: An Experimental Study in Rats

“…The latter are mainly responsible for the formation of new collagen fibrils in the healing zone, being decisive for the anastomotic breaking strength. [14][15][16] No reports of experiments with tensiometery of colonic anastomoses after AZA treatment exist, but tensiometery studies of skin and musculofascial wounds and of bronchial anastomoses after combined AZA and corticosteroid treatment in animals showed significant reduction in breaking strength of cutaneous wounds and of bronchial anastomoses in the postoperative course. 17,18 However, these data cannot simply be transferred to the conditions in the gastrointestinal tract, because the strength of intestinal wounds increases far more rapidly than the strength of cutaneous (and bronchial) wounds.…”

Growth Hormone is a Stimulating but Not An Essential Factor in Healing of Colon

“…Female rats given asubcutaneous dose of 1.0 mg bGH ·kg -1 twice daily reached serum levels of 420-760 μg rhGH ·l -1 after 1 hour,t he concentration was halved after another 2 hours and immeasurable after 8hours (20). This dose was used in the present study sinceithas been shown significantly to accelerate anastomotic healinginnormal rats (9,14). Although, the effect of GH on healing is showne xperimentallyi nd ifferent organsa s wounds, bones, skin and colon it is not known whether the effect is accelerated by GH itself or by stimulation of others factorsa sI GF-I, other anabolich ormones.…”

“…Previously,i th as been shown that GH stimulates the deposition of newly formed collagen in the anas- tomotics egment (9) and promotes the structural reorganization of collagen fibrils in the gap between the cut intestinal edges (14). If the content of collagenis unchanged while an increase in strength is noted, this is explained by apositive modulating effect of GH on the quality of the anastomotic collagen, presumably as an expression of positive effect on the balance between the collagen lysis and synthesis, or as am ark of effect on crosslinking or changed expression of collagensubtypes.…”