Abstract:Due to its low tolerance to external factors such as enzymes, dentin collagen often requires stabilization, which can be achieved through cross-linking. In this study, qualitative and quantitative Fourier transform infrared (FTIR) analyses were used to assess dentin collagen stabilization effects of three structurally-different flavonoids -A-type linkage proanthocyanidins (A-PA), B-type linkage proanthocyanidins (B-PA), and epigallocatechin gallate (EGCG), all from natural extracts. Particularly, transmission … Show more
“…Furthermore, an intriguing observation was the shift of the amide III vibration of the membranes (initially at ~1240 cm −1 ) to a peak at ~1230 cm −1 because of the in-plane bending of C–N and N–H. 37 Furthermore, XPS imaging of collagen membranes after 7 and 14 d of incubation showed the presence of cerium, and the ratio of Ce (IV) to Ce (III) was 54.12%: 45.88% (7 d) and 74.7%: 25.3% (14 d) respectively. Figure 2 TEM, HRTEM, FTIR and XPS images of collagen membranes treated with BMS.…”
Section: Resultsmentioning
confidence: 99%
“…The peak at ~1545 cm −1 of amide II was shifted to ~1490 cm −1 which was associated with C=O stretching, and the peak at ~1640 cm −1 of amide I was shifted to ~1630 cm −1 which was associated with N–H bending and C–N stretching. 37 These were caused by the incorporation of cerium oxide in the mineralizing dentin. XPS analysis of dentin surface ( Figure 3h ) mineralized for 7 and 14 d showed that the ratio of tetravalent to trivalent cerium was 30.4%:69.6% for 7-day mineralized samples and 62.28%:37.72% for 14-day samples.…”
Objective
This study was to investigate a novel antibacterial biomimetic mineralization strategy for exploring its potential application for root canal disinfection when stabilized cerium oxide was used.
Material and Methods
A biomimetic mineralization solution (BMS) consisting of cerium nitrate and dextran was prepared. Single-layer collagen fibrils, collagen membranes, demineralized dentin, and root canal system were treated with the BMS for mineralization. The mineralized samples underwent comprehensive characterization using various techniques, including transmission electron microscopy (TEM), high-resolution TEM (HRTEM), Fourier transform infrared spectroscopy (FTIR), scanning transmission electron microscopy (STEM), selected-area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and micro-CT. Additionally, the antimicrobial properties of the BMS and the remineralized dentin were also analyzed with broth microdilution method, live/dead staining, and SEM.
Results
Cerium ions in the BMS underwent a transformation into cerium oxide nanoparticles, which were deposited in the inter- and intra-fibrillar collagen spaces through a meticulous bottom-up process. XPS analysis disclosed the presence of both Ce (III) and Ce (IV) of the generated cerium oxides. A comprehensive examination utilizing SEM and micro-CT identified the presence of cerium oxide nanoparticles deposited within the dentinal tubules and lateral canals of the root canal system. The BMS and remineralized dentin exhibited substantial antibacterial efficacy against E. faecalis, as substantiated by assessments involving the broth dilution method and live/dead staining technique. The SEM findings revealed the cell morphological changes of deceased E. faecalis.
Conclusion
This study successfully demonstrated antibacterial biomimetic mineralization as well as sealing dentinal tubules and lateral branches of root canals using cerium nitrate and dextran. This novel biomimetic mineralization could be used as an alternative strategy for root canal disinfection.
“…Furthermore, an intriguing observation was the shift of the amide III vibration of the membranes (initially at ~1240 cm −1 ) to a peak at ~1230 cm −1 because of the in-plane bending of C–N and N–H. 37 Furthermore, XPS imaging of collagen membranes after 7 and 14 d of incubation showed the presence of cerium, and the ratio of Ce (IV) to Ce (III) was 54.12%: 45.88% (7 d) and 74.7%: 25.3% (14 d) respectively. Figure 2 TEM, HRTEM, FTIR and XPS images of collagen membranes treated with BMS.…”
Section: Resultsmentioning
confidence: 99%
“…The peak at ~1545 cm −1 of amide II was shifted to ~1490 cm −1 which was associated with C=O stretching, and the peak at ~1640 cm −1 of amide I was shifted to ~1630 cm −1 which was associated with N–H bending and C–N stretching. 37 These were caused by the incorporation of cerium oxide in the mineralizing dentin. XPS analysis of dentin surface ( Figure 3h ) mineralized for 7 and 14 d showed that the ratio of tetravalent to trivalent cerium was 30.4%:69.6% for 7-day mineralized samples and 62.28%:37.72% for 14-day samples.…”
Objective
This study was to investigate a novel antibacterial biomimetic mineralization strategy for exploring its potential application for root canal disinfection when stabilized cerium oxide was used.
Material and Methods
A biomimetic mineralization solution (BMS) consisting of cerium nitrate and dextran was prepared. Single-layer collagen fibrils, collagen membranes, demineralized dentin, and root canal system were treated with the BMS for mineralization. The mineralized samples underwent comprehensive characterization using various techniques, including transmission electron microscopy (TEM), high-resolution TEM (HRTEM), Fourier transform infrared spectroscopy (FTIR), scanning transmission electron microscopy (STEM), selected-area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and micro-CT. Additionally, the antimicrobial properties of the BMS and the remineralized dentin were also analyzed with broth microdilution method, live/dead staining, and SEM.
Results
Cerium ions in the BMS underwent a transformation into cerium oxide nanoparticles, which were deposited in the inter- and intra-fibrillar collagen spaces through a meticulous bottom-up process. XPS analysis disclosed the presence of both Ce (III) and Ce (IV) of the generated cerium oxides. A comprehensive examination utilizing SEM and micro-CT identified the presence of cerium oxide nanoparticles deposited within the dentinal tubules and lateral canals of the root canal system. The BMS and remineralized dentin exhibited substantial antibacterial efficacy against E. faecalis, as substantiated by assessments involving the broth dilution method and live/dead staining technique. The SEM findings revealed the cell morphological changes of deceased E. faecalis.
Conclusion
This study successfully demonstrated antibacterial biomimetic mineralization as well as sealing dentinal tubules and lateral branches of root canals using cerium nitrate and dextran. This novel biomimetic mineralization could be used as an alternative strategy for root canal disinfection.
“…Proanthocyanidins (PA), which make up a significant fraction of the extract we used, have been shown to induce effects both to the secondary and tertiary structure of collagen, and a significant role for hydrogen bonding in the stabilization of collagen by PA has been suggested [48][49][50]. Besides hydrogen bonding, true covalent interactions have been detected by infrared spectroscopy between PA and collagen [51,52]. In particular, they occur through autooxidation of catechol moieties to ortho-quinone groups, available for Schiff base formation with free amine groups on proteins.…”
To endow an implant surface with enhanced properties to ensure an appropriate seal with the host tissue for inflammation/infection resistance, next-generation bone implant collagen–polyphenol nanolayers were built on conventional titanium surfaces through a multilayer approach. X-ray Photoelectron Spectroscopy (XPS) analysis was performed to investigate the chemical arrangement of molecules within the surface layer and to provide an estimate of their thickness. A short-term (2 and 4 weeks) in vivo test of bone implants in a healthy rabbit model was performed to check possible side effects of the soft surface layer on early phases of osteointegration, leading to secondary stability. Results show the building up of the different nanolayers on top of titanium, resulting in a final composite collagen–polyphenol surface and a layer thickness of about 10 nm. In vivo tests performed on machined and state-of-the-art microrough titanium implants do not show significant differences between coated and uncoated samples, as the surface microroughness remains the main driver of bone-to-implant contact. These results confirm that the surface nanolayer does not interfere with the onset and progression of implant osteointegration and prompt the green light for specific investigations of the potential merits of this bioactive coating as an enhancer of the device/tissue seal.
“…This could explain the highest values recorded in groups using the total-etch mode, disregarding the dentine pre-treatment agents used. On the other hand, stable bonds can only be achieved if the adhesive infiltrates uniformly the etched dentine in order to prevent the different degrees of incomplete infiltration (20) that, if happens, a trapped demineralized zone at the bottom of the hybrid layer (HL) will occur (21) .…”
Aim:The current study was performed to investigate the effect of using two nano PA-based cross-linkers on microtensile bond strength of superficial dentin to total-etch and self-etch adhesive modes using two nano proanthocyanidins cross-linkers in an attempt to improve the bond strength.
Materials and Methods:Sixty sound human molars were used in this study. Teeth were divided into3 main groups (n= 20) according to the cross-linker used. Group 1: Teeth treated with 1%nano pine bark extract gel. Group 2: Teeth treated with 1% nano cocoa seed extract gel. Group 3: Teeth had no treatment (control group). Each group was subdivided into two subgroups according to the adhesive system applied total-etch and self-etch. All specimens were subjected to a micro tensile bond strength test.
Results:The highest micro tensile bond strength values were recorded for group 1 either with total-etch mode (42.3±0.99) or with self-etch mode (34.49±1.05) with a significant difference between groups (p ≤ 0.05).
Conclusion:Natural dentin bio modifiers enhanced the micro tensile bond strength while the 1% nano pine bark uniquely affected the micro tensile bond strength.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
