Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton's jelly and menstrual blood stem cells

Artificial skin substitute made of polymeric films are of great demand in the field of skin tissue engineering. We report here the fabrication of carboxymethyl cellulose (CMC) and poly(ethylene glycol) (PEG) blend films by solution casting method for wound healing applications. The physicochemical characteristics and the thermal stability of the films were analyzed. The surface morphology shows crystalline structures with large hexagonal-like platelet crystals of CMC on the surface of the films. Pure CMC films exhibited higher tensile strength than the CMC/PEG blend films. The swelling ratio (SR) of the films was influenced by the pH of Tris–HCL buffer (2.0, 5.0, and 7.0), which increased with increase in pH. The hemocompatibility assay and cytotoxicity test using NIH 3T3 fibroblast cells showed that the films were biocompatible. To evaluate the wound healing efficacy, the films were applied in full-thickness wounds created in normal and diabetic Wistar albino rats. The wounds healed faster with pure CMC film compared to blend films in both normal and diabetic rats, evidenced by intensive collagen formation in histopathological analysis. Thus, the films have potential application in skin regeneration, thereby to restore the structural and functional characteristics of the skin.

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“… 40 The seeding of cells may lead to strengthening of the films due to the secretion of extracellular matrix by the cells. 41 …”

Section: Discussionmentioning

confidence: 99%

Characterization and Evaluation of Carboxymethyl Cellulose-Based Films for Healing of Full-Thickness Wounds in Normal and Diabetic Rats

Basu¹,

Narendrakumar²,

Arunachalam

et al. 2018

ACS Omega

102

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“…Currently, various tissues are under consideration for MSC isolation, including adipose tissue, muscle, amniotic fluid, menstrual blood [ 74 , 75 ], fetal blood [ 76 ], and periodontal ligaments (PDL) [ 61 ]. The human umbilical cord is a promising source of MSCs, as MSCs can be isolated either from the whole umbilical cord [ 16 , 77 ], the umbilical vein subendothelium [ 78 ], or WJ [ 14 , 19 , 74 ]. One group of researchers divided the umbilical cord into three anatomical segments, i.e., the maternal, middle, and fetal segments [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

“…Collagen type I can be isolated from Sprague-Dawley rat tails after processing and pelleting. Genipin has been selectively used for crosslinking collagen scaffolds to improve the stability and mechanical strength of the scaffolds in the culture medium [ 74 ]. Other crosslinkers have also been used, such as glutaraldehyde and formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic Induction of Wharton’s Jelly-Derived Mesenchymal Stem Cell for Bone Regeneration: A Systematic Review

Ansari

Yazid

Sainik

et al. 2018

Stem Cells International

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Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are emerging as a promising source for bone regeneration in the treatment of bone defects. Previous studies have reported the ability of WJ-MSCs to be induced into the osteogenic lineage. The purpose of this review was to systematically assess the potential of WJ-MSC differentiation into the osteogenic lineage. A comprehensive search was conducted in Medline via Ebscohost and Scopus, where relevant studies published between 1961 and 2018 were selected. The main inclusion criteria were that articles must be primary studies published in English evaluating osteogenic induction of WJ-MSCs. The literature search identified 92 related articles, but only 18 articles met the inclusion criteria. These include two animal studies, three articles containing both in vitro and in vivo assessments, and 13 articles on in vitro studies, all of which are discussed in this review. There were two types of osteogenic induction used in these studies, either chemical or physical. The studies demonstrate that WJ-MSCs are able to differentiate into osteogenic lineage and promote osteogenesis. In light of these observations, it is suggested that WJ-MSCs can be a potential source of stem cells for osteogenic induction, as an alternative to bone marrow-derived mesenchymal stem cells.

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“…This protein is non‐cytotoxic, biocompatible, and biodegradable, but has low elasticity and mechanical strength, poor dimensional stability due to swelling in vivo, ability of being cross‐linked to tailor the mechanical, degradation, and water‐uptake properties, and the possibility of an antigenic response . Collagen can be processed in films, fibers, and foams, to engineer various tissues such as bone, cartilage, heart, ligament, and nerve. Collagen is also suitable to produce scaffolds for the culture of mesenchymal stem cells (MSCs) in tissue engineering …”

Section: Natural Polymers and Calcium Phosphate Materials Propertiesmentioning

confidence: 99%

Natural‐Based Nanocomposites for Bone Tissue Engineering and Regenerative Medicine: A Review

2015

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Tissue engineering and regenerative medicine has been providing exciting technologies for the development of functional substitutes aimed to repair and regenerate damaged tissues and organs. Inspired by the hierarchical nature of bone, nanostructured biomaterials are gaining a singular attention for tissue engineering, owing their ability to promote cell adhesion and proliferation, and hence new bone growth, compared with conventional microsized materials. Of particular interest are nanocomposites involving biopolymeric matrices and bioactive nanosized fillers. Biodegradability, high mechanical strength, and osteointegration and formation of ligamentous tissue are properties required for such materials. Biopolymers are advantageous due to their similarities with extracellular matrices, specific degradation rates, and good biological performance. By its turn, calcium phosphates possess favorable osteoconductivity, resorbability, and biocompatibility. Herein, an overview on the available natural polymer/calcium phosphate nanocomposite materials, their design, and properties is presented. Scaffolds, hydrogels, and fibers as biomimetic strategies for tissue engineering, and processing methodologies are described. The specific biological properties of the nanocomposites, as well as their interaction with cells, including the use of bioactive molecules, are highlighted. Nanocomposites in vivo studies using animal models are also reviewed and discussed.

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Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton's jelly and menstrual blood stem cells

Cited by 11 publications

References 35 publications

Characterization and Evaluation of Carboxymethyl Cellulose-Based Films for Healing of Full-Thickness Wounds in Normal and Diabetic Rats

Characterization and Evaluation of Carboxymethyl Cellulose-Based Films for Healing of Full-Thickness Wounds in Normal and Diabetic Rats

Osteogenic Induction of Wharton’s Jelly-Derived Mesenchymal Stem Cell for Bone Regeneration: A Systematic Review

Natural‐Based Nanocomposites for Bone Tissue Engineering and Regenerative Medicine: A Review

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