Collagen Scaffolds Reinforced with Biomimetic Composite Nano-Sized Carbonate-Substituted Hydroxyapatite Crystals and Shaped by Rapid Prototyping to Contain Internal Microchannels

Sachlos, Eleftherios; Gotora, Duce; Czernuszka, Jan T.

doi:10.1089/ten.2006.12.2479

Cited by 146 publications

(92 citation statements)

References 47 publications

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“…30 Because porous collagen sponges lack rigidity that could be required for weight-bearing applications, composites can be developed with additives such as a bone-like calcium phosphate. 31 One of the known limiting factors of traditional high-density 3D culture is that cells in the center of the mass do not survive because of poor exchange of nutrients and dissolved gases. To optimize conditions for engineering of different tissues, we developed a system to perfuse a medium through collagen sponges seeded with different cell types.…”

Section: In Vitro Histogenesismentioning

confidence: 99%

Collagen scaffolds for tissue engineering

2007

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There are two major approaches to tissue engineering for regeneration of tissues and organs. One involves cell‐free materials and/or factors and one involves delivering cells to contribute to the regeneraion process. Of the many scaffold materials being investigated, collagen type I, with selective removal of its telopeptides, has been shown to have many advantageous features for both of these approaches. Highly porous collagen lattice sponges have been used to support in vitro growth of many types of tissues. Use of bioreactors to control in vitro perfusion of medium and to apply hydrostatic fluid pressure has been shown to enhance histogenesis in collagen scaffolds. Collagen sponges have also been developed to contain differentiating‐inducing materials like demineralized bone to stimulate differentiation of cartilage tissue both in vitro and in vivo. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 338–344, 2008.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: In Vitro Histogenesismentioning

confidence: 99%

Collagen scaffolds for tissue engineering

2007

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“…Such syntheses were denoted as ''biologically inspired'' which means they reproduce an ordered pattern and an environment very similar to natural ones [594][595][596]. The biologically inspired biocomposites of collagen and calcium orthophosphates (mainly, apatites) for bone substitute have a long history [29,364,499,[597][598][599][600][601][602][603][604][605][606][607][608][609][610][611][612][613][614][615] and started from the pioneering study by Mittelmeier and Nizard [616], who mixed calcium orthophosphate granules with a collagen web. Such combinations were found to be bioactive, osteoconductive, osteoinductive [29,585,[617][618][619] and, in general, artificial grafts manufactured from this type of the biocomposites are likely to behave similarly to bones and be of more use in surgery than those prepared from any other materials.…”

Section: Biocomposites With Collagenmentioning

confidence: 99%

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Dorozhkin¹

2009

J Mater Sci

283

146

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“…[3,4] However, bone tissue engineering is currently compromised by its inability to produce load-bearing scaffolds. For example, collagen scaffolds are used for a range of osteogenic applications, [5][6][7] but exhibit a compressive strength of ca. 0.034 MPa, [8] approximately three orders of magnitude lower than that of cancellous bone with values between 10 and 50 MPa.…”

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Bone‐like Resorbable Silk‐based Scaffolds for Load‐bearing Osteoregenerative Applications

Collins

Skaer²,

Gheysens³

et al. 2009

Advanced Materials

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The relative paucity of techniques currently available to repair bone tissue necessitates the development of innovative and more effective clinical strategies. [1,2] Of these, the combined integration of macroporous scaffolds, primed human-cell populations, and growth factors to organize and promote tissue formation is a particularly attractive approach. [3,4] However, bone tissue engineering is currently compromised by its inability to produce load-bearing scaffolds. For example, collagen scaffolds are used for a range of osteogenic applications, [5][6][7] but exhibit a compressive strength of ca. 0.034 MPa, [8] approximately three orders of magnitude lower than that of cancellous bone with values between 10 and 50 MPa. On the other hand, pure calcium phosphate mineral-based scaffolds, which currently dominate the commercial bone substitute materials market, lack a fibrillar protein component and are correspondingly brittle, typically failing catastrophically at compressive loads of less than 5 MPa.Recent studies have explored the possibility of replacing collagen with silk-based resorbable implants. [9][10][11][12] Silk is a fibrillar protein with excellent biocompatibility and mechanical strength, [13] and methodologies exist to convert silk fibers into regenerated silk fibroin solutions that can be subsequently reconstituted into macroporous 3D architectures with b-sheet secondary structure by salt leaching, gas foaming, extrusion layering or freeze drying. [9,[13][14][15][16][17][18] Although these scaffolds are potentially suitable for tissue engineering, similarly to collagenbased biomaterials the reconstituted silks are compromised by low mechanical strength, due in part to partial degradation of the native protein structure during fibroin dissolution. Moreover, attempts to impart significant mechanical reinforcement by calcium phosphate mineralization have not been very successful, due to poor adhesion and integration at the protein/mineral interface. [19,20] As a consequence, more advanced uses of silk are currently focused on novel delivery devices for morphogens, cytokines, and cell populations in models of bone defects. [21][22][23][24][25] In contrast, we present herein the first example of a silk/calcium phosphate macroporous scaffold that is load-bearing with mechanical properties comparable to cancellous bone. The mechanical strength is far in excess of other materials previously produced, and is achieved through the use of high-quality silk fibroins [26] and an integrated procedure for gelation, freezing, and mineralization. Moreover, we demonstrate the effectiveness of these load-bearing materials as nonpyrogenic osteoregenerative scaffolds by in vitro and in vivo testing, and suggest that such materials represent a new class of potentially implantable alternatives to the use of allograft and autograft procedures, for example in surgical applications, where immediate load bearing is required.Silk/calcium phosphate macroporous scaffolds were prepared by freezing phosphate-containing aqueous gels...

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Collagen Scaffolds Reinforced with Biomimetic Composite Nano-Sized Carbonate-Substituted Hydroxyapatite Crystals and Shaped by Rapid Prototyping to Contain Internal Microchannels

Cited by 146 publications

References 47 publications

Collagen scaffolds for tissue engineering

Collagen scaffolds for tissue engineering

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Bone‐like Resorbable Silk‐based Scaffolds for Load‐bearing Osteoregenerative Applications

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