Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo

Ryan, Emily J.; Ryan, Alan J.; González-Vázquez, Arlyng; Philippart, Anahí; Ciraldo, Francesca E.; Hobbs, Christopher; Nicolosi, Valeria; Boccaccını, Aldo R.; Kearney, Cathal J.; O’Brien, Fergal J.

doi:10.1016/j.biomaterials.2019.01.031

Cited by 164 publications

(128 citation statements)

References 77 publications

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“…133,135 Similarly, Ryan et al developed a Cu-doped bioactive glass scaffold to stimulate bone regeneration. 134 In this study, they demonstrated that Cu induced osteogenic differentiation of MSCs through promoting collagen maturation by lysyl oxidase crosslinking. Autefage et al designed a porous scaffold to achieve controlled release of Sr, which induced tissue infiltration and encouraged bone formation.…”

Section: Controlled Release Of Active Chemical Components For Bone Tementioning

confidence: 70%

“…This novel magnetic construct is highly promising for bone regeneration, especially γIONP-CPC, because the internalized magnetic γIONPs inside the cell membrane reoriented and distorted, resulting in an alteration of the cell cycles and differentiation. Additionally, some metal ions such as calcium (Ca), magnesium (Mg), 132,133 strontium (Sr), 121 and copper (Cu) 134 , which mediate chemobiological homeostasis of human, are widely applied in chemical modifications on bone substitute scaffolds to stimulate the osteogenesis and angiogenesis. Minardi et al added Mg to the HA/Col I composite and showed that cells seeded in vivo in the scaffold retained high viability and reproducibility for mature cortical bone formation.…”

Section: Controlled Release Of Active Chemical Components For Bone Tementioning

confidence: 99%

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Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

2020

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Natural bone is a mineralized biological material, which serves a supportive and protective framework for the body, stores minerals for metabolism, and produces blood cells nourishing the body. Normally, bone has an innate capacity to heal from damage. However, massive bone defects due to traumatic injury, tumor resection, or congenital diseases pose a great challenge to reconstructive surgery. Scaffold-based tissue engineering (TE) is a promising strategy for bone regenerative medicine, because biomaterial scaffolds show advanced mechanical properties and a good degradation profile, as well as the feasibility of controlled release of growth and differentiation factors or immobilizing them on the material surface. Additionally, the defined structure of biomaterial scaffolds, as a kind of mechanical cue, can influence cell behaviors, modulate local microenvironment and control key features at the molecular and cellular levels. Recently, nano/micro-assisted regenerative medicine becomes a promising application of TE for the reconstruction of bone defects. For this reason, it is necessary for us to have in-depth knowledge of the development of novel nano/micro-based biomaterial scaffolds. Thus, we herein review the hierarchical structure of bone, and the potential application of nano/micro technologies to guide the design of novel biomaterial structures for bone repair and regeneration.

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Section: Controlled Release Of Active Chemical Components For Bone Tementioning

confidence: 70%

Section: Controlled Release Of Active Chemical Components For Bone Tementioning

confidence: 99%

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

2020

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“…CAM assay was used to investigate scaffolds' angiogenic potential in vivo, as previously described (Ryan et al, 2019). All experimentation carried out on chick embryos was in accordance with the EU Directive 2010/63/EU for animal experiments.…”

Section: Chick Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

“…As a final way to test angiogenic/vascularization properties, we challenged our scaffolds in the CAM assay. Since the CAM is highly vascularised, and chick embryos only become immunocompetent by day 18 of development, the CAM assay has been extensively used to test the angiogenic potential of scaffolds without inducing an immune response (Azzarello et al, 2007;Fishman et al, 2013;Li et al, 2015;Woloszyk et al, 2016;Moreno-Jimenez et al, 2017;Ryan et al, 2019). In this work, we directly compared collagen-GAG ( Figure 8G) with composite scaffolds (Figure 8H).…”

Section: Composite Scaffolds Promoted Greater Angiogenesis In Vivo Comentioning

confidence: 99%

Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential

Amaral

Zayed

Pascu

et al. 2019

Front. Bioeng. Biotechnol.

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Porous collagen-glycosaminoglycan (collagen-GAG) scaffolds have shown promising clinical results for wound healing; however, these scaffolds do not replace the dermal and epidermal layer simultaneously and rely on local endogenous signaling to direct healing. Functionalizing collagen-GAG scaffolds with signaling factors, and/or additional matrix molecules, could help overcome these challenges. An ideal candidate for this is platelet-rich plasma (PRP) as it is a natural reservoir of growth factors, can be activated to form a fibrin gel, and is available intraoperatively. We tested the factors released from PRP (PRPr) and found that at specific concentrations, PRPr enhanced cell proliferation and migration and induced angiogenesis to a greater extent than fetal bovine serum (FBS) controls. This motivated us to develop a strategy to successfully incorporate PRP homogeneously within the pores of the collagen-GAG scaffolds. The composite scaffold released key growth factors for wound healing (FGF, TGFβ) and vascularization (VEGF, PDGF) for up to 14 days. In addition, the composite scaffold had enhanced mechanical properties (when compared to PRP gel alone), while providing a continuous upper surface of extracellular matrix (ECM) for keratinocyte seeding. The levels of the factors released from the composite scaffold were sufficient to sustain proliferation of key cells involved in wound healing, including human endothelial cells, mesenchymal stromal cells, fibroblasts, and keratinocytes; even in the absence of FBS supplementation. In functional in vitro and in vivo vascularization assays, our composite scaffold demonstrated increased angiogenic and vascularization potential, which is known to lead to enhanced wound healing. Upon pro-inflammatory induction, macrophages released lower levels of the pro-inflammatory marker MIP-1α when treated with PRPr; and released higher levels of the anti-inflammatory marker IL1-ra upon both pro-and anti-inflammatory induction when treated with the composite scaffold. Finally, our composite scaffold supported a co-culture system of human fibroblasts and do Amaral et al. PRP Incorporation Into Collagen-Based Scaffold keratinocytes that resulted in an epidermal-like layer, with keratinocytes constrained to the surface of the scaffold; by contrast, keratinocytes were observed infiltrating the PRPfree scaffold. This novel composite scaffold has the potential for rapid translation to the clinic by isolating PRP from a patient intraoperatively and combining it with regulatory approved scaffolds to enhance wound repair.

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“…As previously noted, EDS measurements also confirm the presence of Cu in the mWEDM treated surface (figure 4). Cu has been proven to have good anti-bacterial and anti-infection capabilities, enhancing alkaline phosphatase activity, and increasing the bone apposition rate in the early phases of osteogenesis [48,49]. It is believed that the heavy metal ions such as Cu 2+ can kill bacteria by destroying their proteins [50].…”

Section: Microbiological Studiesmentioning

confidence: 99%

Advanced surface treatment techniques counteract biofilm-associated infections on dental implants

Koopaie

Bordbar‐Khiabani

Kolahdooz

et al. 2020

Mater. Res. Express

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Topography and surface chemistry can significantly affect biofilm formation on dental implants. Recently, the γ-TiAl alloy was considered as the most reliable candidates for the preparation of dental implants because of its excellent mechanical strength, chemical stability and biocompatibility. The emphasis of this study lies in the effects of high-speed milling assisted the minimum quantity of lubrication (HSM-MQL), micro-current wire electrical discharge machining (mWEDM), Er,Cr:YSGG laser and sandblasting/largegrit/acid-etching (SLA) treatments on surface morphology, topography, chemical composition, wettability and biofilm-associated infections on the surface of each group. The surface-treated samples were analyzed using a scanning electron microscope (SEM), SEM surface reconstruction, energy dispersive x-ray spectroscopy (EDS) and water contact angle measuring system. SEM and topography images of mWEDM and laser-treated surfaces showed more irregular surfaces compared to SLA and HSM-MQL surfaces. Results showed that mWEDM and laser-treated surfaces revealed hydrophobic behavior. A significant decrease of biofilm formation was observed on mWEDM treated surface due to the hydrophobicity and existence of the copper element in the recast layer chemical composition. Moreover, EDS confirmed that the zirconium, silicon, and fluorine elements were decorated onto the SLA treated γ-TiAl surface that can have a direct effect on the anti-bacterial activity.

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Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo

Cited by 164 publications

References 77 publications

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential

Advanced surface treatment techniques counteract biofilm-associated infections on dental implants

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