Collagen Scaffold and Lipoaspirate Fluid-derived Stem Cells for the Treatment of Cartilage Defects in a Rabbit Model

Many methods of stem cells collection and isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. The present study purposed to describe a minimally invasive surgical protocol for adipose tissue collection from sheep�s inter-scapular area. The procedure was carried out on adult sheeps, in aseptic conditions. A light sedation protocol with Detomidine was performed, the recovery from anesthesia being carried out with Atipamezole. Throughout the sedation, the surgical procedure and the recovery from anesthesia, the vital functions of the animal were monitored. The adipose tissue samples collected in sterile tubes with culture medium (Dulbecco�s modified Eagle�s medium - DMEM/10% / FBS10 - fetal bovine serum, 2% antibiotic/antifungal), have been succesfully used by our research team for adipose tissue derived stem cells (ADSCs) isolation for further use in cardiac valves tissue engineering.

Section: Abstract: Adipose Tissue Sheep Stem Cells Detomidine Atimentioning

confidence: 99%

Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model

Hussein¹,

Harpa²,

Movileanu³

et al. 2019

“…In clinical practice, many surgical interventions need the support given by the synthetic biomaterials. Also, the various aspects related to the interface between synthetic biomaterials and human tissue appear to be interesting not just for neurosurgery [1,2], but also for many clinical specializations like orthopedics [3][4][5][6][7], dentistry [8][9][10], abdominal surgery [11][12][13][14], gynecology [15][16][17][18], cardiovascular surger y [19,20], and ophthalmology [21].…”

mentioning

confidence: 99%

Influence of the Bone Cements Processing on the Mechanical Properties in Cranioplasty

Riviș¹,

Pricop²,

Talpoș³

et al. 2018

Self Cite

The aim of this study is to observe the time of mixing influence on the properties of commercially available PMMA bone cement, widely used in cranioplasty. The studied bone cement is provided in the form of a solid powder (the copolymer) and a liquid monomer. The increase of the mixing phase duration and the use of two mixing methods (manual and mechanical) effect on surface and mechanical characteristics were studied. The samples were prepared as if in the operation room. Surface characteristics were studied by means of contact angle measurements, morphology by scanning electron microscopy (SEM) and mechanical characteristics determined by flexural tests in a three point bending configuration. The conclusion of this study is that by using a mechanical mixing method and increasing mixing time higher flexural strength can be achieved by reducing pore content within bone cement.

“…The relevance of the adhesion phenomena is important for various clinical specializations like cardiovascular surgery [1], orthopedics [2][3][4][5], dentistry [6][7][8][9], neurosurgery [10,11], abdominal surgery [12][13][14][15], gynecology [16,17], and ophthalmology [18].…”

mentioning

confidence: 99%

Bioadhesives Used in Cardiovascular Surgery

Moldovan¹,

Gheorghiță²,

Antoniac³

et al. 2018

Self Cite