Collagen remodelling in the guinea-pig uterine cervix at term is associated with a decrease in progesterone receptor expression

Rodríguez, Horacio Adolfo; Kass, Laura; Varayoud, Jorgelina; Ramos, Jorge G.; Ortega, Hugo Héctor; Durando, Milena; Muñoz-de-Toro, Mónica; Luque, Enrique H.

doi:10.1093/molehr/gag099

Cited by 37 publications

(42 citation statements)

References 38 publications

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“…The uterine cervix undergoes dramatic adaptation processes during pregnancy, parturition and postpartum (Shi et al 2000, Montes et al 2002, Rodríguez et al 2003. There are striking changes in uterine vascular permeability, growth, density, vasodilatation and blood flow as a physiological response to cellular modifications, especially during pregnancy (Pavelock et al 2001, Reynolds et al 2002.…”

Section: Discussionmentioning

confidence: 99%

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Varayoud¹,

Ramos²,

Bosquiazzo³

et al. 2004

Reproduction

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During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and a-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.

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Section: Discussionmentioning

confidence: 99%

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Varayoud¹,

Ramos²,

Bosquiazzo³

et al. 2004

Reproduction

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“…The feasibility of using commercial kits prepared for human plasma E 2 and P 4 determination in guinea pigs has previously been validated by others (Rodriguez et al 2003a;Rodriguez et al 2003b). The maximal interassay coefficient of variation for P 4 was 9.8% and for E 2 it was 7.5%.…”

Section: Methodsmentioning

confidence: 98%

The effect of deslorelin acetate on the oestrous cycle of female guinea pigs

Kohutova¹,

Jekl²,

Knotek³

et al. 2015

Vet. Med. - Czech

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Deslorelin acetate, a GnRH agonist, is commonly used to prevent folliculogenesis in several species. However, little is known of the effect of deslorelin acetate implants on the oestrous cycle of female guinea pigs. Fifteen intact healthy female guinea pigs were investigated in this study. Signs of sexual behaviour, the presence of a vaginal membrane along with plasma concentrations of oestradiol (E 2 ) and progesterone (P 4 ), were monitored during two consecutive oestrous cycles. At the beginning of the third oestrous cycle each guinea pig was administered an implant of the GnRH analogue, deslorelin acetate, (4.7 mg). When compared to the untreated state, deslorelin implantation was associated with altered signs of oestrus. The average time to opening of the vaginal membrane was delayed. After opening, the vaginas were found to be variably opened and closed. A significant reduction in P 4 (to less than 1.0 ng/ml) and cessation of P 4 cyclical variation was observed. Plasma E 2 remained high during the whole experimental period. This study shows that cessation of the oestrous cycle by the deslorelin implant might be useful in preventing pregnancy in guinea pigs.Keywords: guinea pig; deslorelin; progesterone; oestradiol; oestrous cycle List of abbreviations E 2 = oestradiol, FSH = follicle-stimulating hormone, GnRH = gonadotropin-releasing hormone, gpGnRH = guinea pig gonadotropin-releasing hormone, LH = luteinising hormone, P 4 = progesterone

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“…For granulosa cell proliferation, we used an anti-Ki67 affinitypurified rabbit polyclonal antibody generated and tested in our laboratory (Varayoud et al 2008, Rivera et al 2011. The specificity of each antibody was tested using western blot analysis of protein extracts (Rodríguez et al 2003) obtained from intact uterine or gonad samples of ewes (data not shown). Each immunohistochemical run included positive tissues and negative controls replacing the primary antibody with nonimmune serum (Sigma-Aldrich).…”

Section: Immunohistochemistry and Tunel Assaymentioning

confidence: 99%

Neonatal exposure to xenoestrogens impairs the ovarian response to gonadotropin treatment in lambs

Rivera¹,

Varayoud²,

Rodríguez³

et al. 2015

Reproduction

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Bisphenol A (BPA) and diethylstilbestrol (DES) are xenoestrogens, which have been associated with altered effects on reproduction. We hypothesized that neonatal xenoestrogen exposure affects the ovarian functionality in lambs. Thus, we evaluated the ovarian response to exogenous ovine FSH (oFSH) administered from postnatal day 30 (PND30) to PND32 in female lambs previously exposed to low doses of DES or BPA (BPA50: 50 mg/kg per day, BPA0.5: 0.5 mg/kg per day) from PND1 to PND14. We determined: i) follicular growth, ii) circulating levels of 17b-estradiol (E 2 ), iii) steroid receptors (estrogen receptor alpha, estrogen receptor beta, and androgen receptor (AR)) and atresia, and iv) mRNA expression levels of the ovarian bone morphogenetic protein (BMPs) system (BMP6, BMP15, BMPR1B, and GDF9) and FSH receptor (FSHR). Lambs neonatally exposed to DES or BPA showed an impaired ovarian response to oFSH with a lower number of follicles R2 mm in diameter together with a lower number of atretic follicles and no increase in E 2 serum levels in response to oFSH treatment. In addition, AR induction by oFSH was disrupted in granulosa and theca cells of lambs exposed to DES or BPA. An increase in GDF9 mRNA expression levels was observed in oFSH-primed lambs previously treated with DES or BPA50. In contrast, a decrease in BMPR1B was observed in BPA0.5-postnatally exposed lambs. The modifications in AR, GDF9, and BMPR1B may be associated with the altered ovarian function due to neonatal xenoestrogen exposure in response to an exogenous gonadotropin stimulus. These alterations may be the pathophysiological basis of subfertility syndrome in adulthood.Reproduction (2015) 149 645-655

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Collagen remodelling in the guinea-pig uterine cervix at term is associated with a decrease in progesterone receptor expression

Cited by 37 publications

References 38 publications

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

The effect of deslorelin acetate on the oestrous cycle of female guinea pigs

Neonatal exposure to xenoestrogens impairs the ovarian response to gonadotropin treatment in lambs

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