Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization

Abstract

Ulcerative colitis (UC) is an inflammatory bowel disease caused by mucosal immune system disorder, which has increased steadily all over the world. Previous studies have shown that collagen peptide (CP) has various beneficial biological activities, it is not clear whether the effect of CP on UC is positive or negative. In this study, 2.5% dextran sulfate sodium (DSS) was used to establish acute colitis in mice. Our results suggested that CP supplementation (200, 400 mg/kg/day) promoted the progression of colitis, increased the expression of inflammatory factors and the infiltration of colonic lamina propria macrophages. Gut microbiota analysis showed the composition changed significantly and inflammation promoted bacteria was after CP treatment. Meanwhile, the effect of CP on macrophage polarization was further determined in Raw264.7 cell line. The results showed that CP treatment could increase the polarization of M1 macrophages and promote the expression of inflammatory factors. In conclusion, our results showed that CP treatment could disrupt the gut microbiota of host, promote macrophage activation and aggravate DSS-induced colitis. This may suggest that patients with intestinal inflammation should not take marine derived CP.

C-Phycocyanin Ameliorates the Senescence of Mesenchymal Stem Cells through ZDHHC5-Mediated Autophagy via PI3K/AKT/mTOR Pathway

“…The level of MDA and the activity of SOD in serum were also improved by C-PC [ 27 ]. The gut microbiota may also be involved in these inflammation diseases [ 42 ]. Altogether, it’s a complex process to delay senility in vivo for C-PC which needs further research in future.…”

Preventive effect of tilapia skin collagen hydrolysates on ulcerative colitis mice based on metabonomic and 16 S rRNA gene sequencing

“…This leads to the transmembrane pattern recognition receptors of bacteria being revitalized, which disrupts the balance of immunity, eventually activating the inflammatory pathways of NF‐κB, Akt, and MAPK, and stimulating the discharge of inflammatory cytokines, promoting immune response, and damage to the intestinal mucosal barrier 52‐55 . IL‐6 plays a significant regulatory role in the pathogenesis of UC, which can activate the STAT3 and NF‐κB signaling pathway that further increase the permeability of epithelial cells and enhance intestinal mucosal injury 56,57 . NF‐κB is considered to play a key role in the release of proinflammatory mediators; it has been reported that colonic inflammation can be alleviated by inhibiting the translocation of p65 from the cytosol to the nucleus, the phosphorylation and degradation of IκB‐α, and suppression of the expressions of NF‐κB‐related proteins 58 .…”

“…[52][53][54][55] IL-6 plays a significant regulatory role in the pathogenesis of UC, which can activate the STAT3 and NF-κB signaling pathway that further increase the permeability of epithelial cells and enhance intestinal mucosal injury. 56,57 NF-κB is considered to play a key role in the release of proinflammatory mediators; it has been reported that colonic inflammation can be alleviated by inhibiting the translocation of p65 from the cytosol to the nucleus, the phosphorylation and degradation of IκB-⊍, and suppression of the expressions of NF-κB-related proteins. 58 IL-6 was significantly increased in mice with DSS-induced colitis, which might be involved in the regulation of signaling pathways such as apoptosis, migration, and protein synthesis.…”

Glycated Casein by TGase-Type Exerts Protection Potential against DSS-Induced Colitis via Inhibiting TLR4/NF-κB Signaling Pathways in C57BL/6J Mice

“…Green tea extract containing piper retrofractum fruit inhibits DSS-induced colonic inflammation by reducing the expression of miR-21 and NF-κB [ 25 ]. Collagen peptide promotes DSS-induced colitis by disrupting the gut microbiota and controlling macrophage polarization [ 26 ]. The Maillard product of ovalbumin blocks the release of lysine and essential amino acids, as well as improves the gut microbiota and levels of short-chain fatty acids in vivo [ 11 ].…”