Collagen Modulates Gene Activation of Plasminogen Activator System Molecules

Jones, Jenny M.; Cohen, Rhonna L.; Chambers, Donald A.

doi:10.1006/excr.2002.5644

Cited by 14 publications

(10 citation statements)

References 57 publications

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“…An increased vascular permeability after a single MED is, however, possible because the VEGF mRNA level tends to be increased (P = 0.09). As reported by Marionnet et al (29), in human epidermis after UV-B exposure, a single MED of SSR increased the PAI-1 mRNA level in human skin in vivo, and this might be the result of collagen degradation (19).…”

Section: Discussionsupporting

confidence: 61%

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Seité¹,

Colige²,

Deroanne³

et al. 2004

Photochem Photobiol

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Damage to the skin extracellular matrix (ECM) is the hallmark of long-term exposure to solar UV radiation. The aim of our study was to investigate the changes induced in unexposed human skin in vivo after single or repeated (five times a week for 6 weeks) exposure to 1 minimal erythemal dose (MED) of UV solar-simulated radiation. Morphological and biochemical analyses were used to evaluate the structural ECM components and the balance between the degrading enzymes and their physiologic inhibitors. A three-fold increase in matrix metalloproteina.se 2 messenger RNA (mRNA) (P < 0.02, unexposed versus exposed) was observed after both single and repeated exposures. Fibrillin 1 mRNA level was increased by chronic exposure (P < 0.02) and unaltered by a single MED. On the contrary, a single MED significantly enhanced mRNA levels of interleukin-1α (IL-1α), IL-1β (P < 0.02) and plasminogen activator inhibitor-1 (P < 0.05). Immunohisto-chemistry demonstrated a significant decrease in Type-I procollagen localized just below the dermal-epidermal junction in both types of exposed sites. At the same location, the immunodetected tenascin was significantly enhanced, whereas a slight increase in Type-IΠ procollagen deposits was also observed in chronically exposed areas. Although we were unable to observe any change in elastic fibers in chronically exposed buttock skin, a significant increase in lysozyme and alpha-1 antitrypsin deposits on these fibers was observed. These results demonstrate the existence of a differential regulation, after chronic exposure compared with an acute one, of some ECM components and inflammatory mediators.

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Section: Discussionsupporting

confidence: 61%

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Seité¹,

Colige²,

Deroanne³

et al. 2004

Photochem Photobiol

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“…MTMMPs are likely to activate pro-MMPs (Sato et al 1994;Okada et al 1997). Plasminogen activators (serine proteinases) also induce active MMPs (Jones et al 2002). The expression of MMP-2/MT 1-MMP is also increased in endothelial tubulogenesis in fibrin gel (Lafleur et al 2002).…”

Section: Discussionmentioning

confidence: 97%

Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture

et al. 2004

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In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odontoblast differentiation (Kikuchi et al. 2002), we examined whether dental papilla cells in rat mandibular incisors could differentiate into tubular dentine-forming cells. In our previously established organ culture system, CM-Dil-labeled cells that were microinjected into isolated dental papillae were replaced by a row of odontoblasts. In a three-dimensional cell culture system, which consists of two kinds of type I collagen in the upper layer over multi-layered cells seeded onto collagen containing Matrigel in the lower layer and which acts as a structural meshwork, dental papilla cells were incubated as multi-layered cells in an artificial extracellular matrix (ECM). The cells aggregated to form a cell mass and invaginated as a cell mass into the ECM. The cells also extended fine fibrillar processes into the ECM. With regard to invagination, the proteolytic activities of matrix metalloproteinase-2 (MMP-2)/membrane type 1-matrix metalloproteinase (MT 1-MMP) were observed on the outer multi-layers of cells within a cell mass adjacent to the ECM. The cell mass progressively shrank to about one-half to one-third of its original diameter and was organized as a tissue surrounded by a newly secreted ECM, like dental pulp-dentine. The cells adjacent to the secreted ECM were constructed as a row of polarized columnar cells. They extended slender processes into the new ECM, which is characteristic of tubular matrix. Dentine sialophosphoprotein (DSPP) and dentine matrix protein 1 (DMP 1) genes, which are specific for odontoblast differentiation, were expressed in an aggregated cell mass where tubular matrix-forming cells were induced. Furthermore, the tubular matrix became mineralized under prolonged culture. These results imply that the putative progenitor cells/stem cells residing in dental papillae can differentiate into odontoblasts under appropriate conditions in vitro.

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“…For stimulation experiments, the same number of keratinocytes (between 10 000 and 15 000 cells/cm 2 ) was added to each well of the 24‐well plates without collagen I coating (Becton Dickinson Labware) and grown to 70–80% confluence in KGM. Since collagen I‐coating has previously been reported to modulate tPA mRNA expression [28] GABEB keratinocytes were kept in 24‐well plates without collagen I‐coating for at least 48 h before stimulation experiments were initiated. Hydrocortisone was omitted 12 h prior to stimulation to exclude interference with PA production [29].…”

Section: Methodsmentioning

confidence: 99%

Elevated expression and release of tissue-type, but not urokinase-type, plasminogen activator after binding of autoantibodies to bullous pemphigoid antigen 180 in cultured human keratinocytes

Schmidt

Wehr

Tabengwa

et al. 2004

Clinical and Experimental Immunology

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SUMMARYIn bullous pemphigoid (BP), the binding of BP180-specific antibodies to their hemidesmosomal target antigen is not sufficient for blister formation, but must be accompanied by the release of proteases. Using plasminogen activator (PA) knock-out mice, the PA system has previously been shown to be a prerequisite for blister formation in experimental murine BP. Here, we found elevated levels of plasmin and tPA, but not of uPA, in blister fluid from BP patients ( n = 7) compared to blisters from patients with toxic epidermal necrolysis ( n = 4) and suction blisters in healthy controls ( n = 7). Subsequently, we addressed the question whether keratinocytes release PA in response to the binding of anti-BP180 antibodies. Treatment of cultured normal human keratinocytes with BP IgG, but not with control IgG, led to both increased protein and mRNA levels of tPA, but not of uPA, as determined by ELISA and RT-PCR, respectively. The specificity of this finding was confirmed using BP180-deficient keratinocytes from a patient with generalized atrophic benign epidermolysis bullosa, where no tPA release was observed after stimulation with BP IgG. Our results show the elevated expression and release of tPA from normal human keratinocytes upon stimulation with antibodies to human BP180. Keratinocytes, by secreting tPA, may thus play an active role in blister formation of BP.

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Collagen Modulates Gene Activation of Plasminogen Activator System Molecules

Cited by 14 publications

References 57 publications

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Changes in Matrix Gene and Protein Expressions After Single or Repeated Exposure to One Minimal Erythemal Dose of Solar-simulated Radiation in Human Skin In Vivo

Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture

Elevated expression and release of tissue-type, but not urokinase-type, plasminogen activator after binding of autoantibodies to bullous pemphigoid antigen 180 in cultured human keratinocytes

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