Collagen minipellet as a controlled release delivery system for tetanus and diphtheria toxoid

Implants as Sustained Release Delivery Devices for Vaccine Antigens

“…The authors were able to show that the cylindrical-shaped implants degraded within 35 days of implantation. Surprisingly, only a few studies can be found in literature using this type of polymer: the studies by Lofthouse and co-workers and a study by Higaki et al using tetanus and diphtheria toxoids (Higaki et al 2001 ). One more study by Ochiya et al utilized a collagen minipellet for the controlled delivery of plasmid DNA to mice (Ochiya et al 1999 ).…”

“…For example, Higaki et al ( 2001 ) used a lyophilized cake of collagen containing the antigens tetanus toxoid or diphtheria toxoid, and added a small amount of distilled water to achieve a swelling of the lyophilized matrix (Higaki et al 2001 ). The spongy mass was then passed through a nozzle with an inner diameter of 1.7 mm and the resulting rod-shaped implants were air dried at 4 °C in a humid atmosphere for 24 h. Similarly, Yamagata et al utilized a melted mixture of monoglycerides and lyophilized IFN-α which was loaded into a 14 G stainless steel needle and extruded to form a rod with a diameter of 1.2 mm (Yamagata et al 2000 ).…”

Microspheres for Drug Delivery

“…In recent years, much work has focused on developing microsphere-based, single-administration, vaccine delivery vehicles [44,45,149,158] using a variety of materials including hydroxypropyl cellulose/PLG [101], poly(ε-caprolactone) [160], PLA [141], chitosan [31], and collagen [74], though the majority have been fabricated with PLG [7,44,47,158,162]. Maintenance of antigen bioactivity has been problematic due to contact of the proteins with organic solvents and the hydrophobic polymer, and the use of strong physical forces to produce the microspheres [2,36,81].…”

Influence of systemic administration of atelocollagen on mouse livers: an ideal biomaterial for systemic drug delivery

“…During the last decade, studies have shown that AC is an efficient carrier of toxoid vaccines (Higaki et al, 2001), cytokines (interferon, bFGF, G-CSF, BMP-2) (Maeda et al, 1996;Hosokawa et al, 2000;Fujimura et al, 1995), plasmid DNA (Ochiya et al, 1999;Sano et al, 2003), siRNA (Honma et al, 2008), microRNA (Tazawa et al, 2007), and antisense oligonucleotides (Nakazawa et al, 2007). AC may function as an efficient DDS carrier by binding and forming a complex with compounds through intermolecular forces, and thereby protecting these compounds from degradation and/or releasing them slowly in target tissues or cells.…”