Collagen mimetic peptide-conjugated photopolymerizable PEG hydrogel

Lee, H. Janice; Lee, Jin Soo; Chansakul, Thanissara; Yu, Christopher; Elisseeff, Jennifer H.; Yu, S. Michael

doi:10.1016/j.biomaterials.2006.06.001

Cited by 180 publications

(154 citation statements)

References 37 publications

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“…This led to the discovery of CMP's structure-dependent binding affinity to type I collagen which has been applied to the fabrication of collagen substrates that repel cell attachment 19 and a poly(ethyleneglycol)-collagen hybrid scaffold ideal for chondrocyte and mesenchymal stem cell encapsulation. 24 In this article, we present temperature-dependent collagen binding/ release studies and nanoparticle localization experiments of a series of CMPs that provide new insights into the mechanism of CMP-collagen interactions. We also provide histological analyses of human liver tissue using labeled CMPs that highlight CMP's remarkable binding specificity to human collagens.…”

Section: Introductionmentioning

confidence: 99%

Spatio-Temporal Modification of Collagen Scaffolds Mediated by Triple Helical Propensity

et al. 2008

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Functionalized collagen that incorporates exogenous compounds may offer new and improved biomaterials applications, especially in drug-delivery, multifunctional implants, and tissue engineering. To that end, we developed a specific and reversible collagen modification technique utilizing associative chain interactions between synthetic collagen mimetic peptide (CMP) [(ProHypGly) x ; Hyp = hydroxyproline] and type I collagen. Here we show temperature-dependent collagen binding and subsequent release of a series of CMPs with varying chain lengths indicating a triple helical propensity driven binding mechanism. The binding took place when melted, singlestrand CMPs were allowed to fold while in contact with reconstituted type I collagens. The binding affinity is highly specific to collagen as labeled CMP bound to nanometer scale periodic positions on type I collagen fibers and could be used to selectively image collagens in ex vivo human liver tissue. When heated to physiological temperature, bound CMPs discharged from the collagen at a sustained rate that correlated with CMP's triple helical propensity, suggesting that sustainability is mediated by dynamic collagen-CMP interactions. We also report on the spatially defined modification of collagen film with linear and multi-arm poly(ethylene glycol)-CMP conjugates; at 37 °C, these PEG-CMP conjugates exhibited temporary cell repelling activity lasting up to 9 days. These results demonstrate new opportunities for targeting pathologic collagens for diagnostic or therapeutic applications and for fabricating multifunctional collagen coatings and scaffolds that can temporally and spatially control the behavior of cells associated with the collagen matrices.

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Section: Introductionmentioning

confidence: 99%

Spatio-Temporal Modification of Collagen Scaffolds Mediated by Triple Helical Propensity

et al. 2008

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“…PEG-diacrylate (PEGDA) is a photopolymerizable hydrogel that cross-links in presence of an initiator under UV light. PEGDA has been extensively used in cartilage tissue engineering as an inert encapsulating system or after modification with different peptides, growth factors, or in semi-interpenetrating networks with ECM proteins (Hwang et al, 2006;Lee et al, 2006;Sharma et al, 2007). It is a reproducible hydrogel system with fine-tunable physicochemical and mechanical properties that enable tissue regeneration without the potential risks associated to natural polymers, thus making it a promising candidate for clinical use.…”

Section: Biomaterials and Scaffold Fabrication Technologiesmentioning

confidence: 99%

Joint Cartilage Tissue Engineering and Pre-Clinical Safety and Efficacy Testing

Koch¹,

Moroni²,

Leysi-Derilou³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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“…Hydrogels were lyophilized for 48 h, and their dry weights were measured. Next, dry constructs were digested with papain (Worthington Biomedical, Lakewood, NJ) overnight [15]. Hoechst 33258 dye (Molecular Probes, Eugene, OR) was used for the DNA assay as previously described [16].…”

Section: Biochemical Assays Of Esdc-encapsulated Hydrogelmentioning

confidence: 99%