Collagen maturity and mineralization in mesenchymal stem cells cultured on the hydroxyapatite-based bone scaffold analyzed by ATR-FTIR spectroscopic imaging

Gieroba, Barbara; Przekora, Agata; Kalisz, Grzegorz; Kazimierczak, Paulina; Song, Cai Li; Wójcik, Michał; Ginalska, Grażyna; Kazarian, Sergei G.; Sroka-Bartnicka, Anna

doi:10.1016/j.msec.2020.111634

Cited by 34 publications

(24 citation statements)

References 59 publications

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“…There are positively weighted peaks around 1176cm -1 , 1130cm -1 , 1085cm -1 and 1043cm -1 , of which 1176cm -1 was related to the vibration band of sugar-phosphate, 1130cm -1 was assigned to the C=O stretching vibration of ribose in RNA ( 32 ), 1085cm -1 was ascribed to the symmetric phosphate vibrations ( 35 ), 1043cm -1 was attributed to the stretching vibration and bending vibration of C-O in carbohydrates ( 14 ). Two positively weighted peaks at 1226cm -1 and 1026cm -1 , of which 1226cm -1 was due to the asymmetric stretching vibration of

in nucleic acids ( 36 ), 1026cm -1 was related to the stretching vibration of C-O and bending vibration of C-H in aromatic amino acids ( 37 ). Therefore, the loading plot of Factor-1 showed that

in nucleic acids play a key role in distinguishing the serum patients with lung cancer from that of healthy people.…”

Section: Resultsmentioning

confidence: 99%

Diagnosis of Lung Cancer by ATR-FTIR Spectroscopy and Chemometrics

Yang

Qian

et al. 2021

Front. Oncol.

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Lung cancer is the leading cause of cancer-related death in the world. Early diagnosis has great significance for the survival of patients with lung cancer. In this paper, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy combined with chemometrics was used to study the serum samples from patients with lung cancer and healthy people. The results of spectral band area comparison showed that the concentrations of protein, lipid and nucleic acids molecules in serum of patients with lung cancer were increased compared with those in healthy people. The original spectra were preprocessed to improve the accuracy of principal component regression (PCR) and partial least squares-discriminant analysis (PLS-DA) models. PLS-DA results for first derivative spectral data in nucleic acids (1250-1000cm-1) band showed 80% sensitivity, 91.89% specificity and 87.10% accuracy with high Rc2 of 0.8949 and Rv2 of 0.8153, low RMSEC of 0.3136 and RMSEV of 0.4180. It is shown that ATR-FTIR spectroscopy combined with chemometrics might be developed as a simple method for clinical screening and diagnosis of lung cancer.

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in nucleic acids ( 36 ), 1026cm -1 was related to the stretching vibration of C-O and bending vibration of C-H in aromatic amino acids ( 37 ). Therefore, the loading plot of Factor-1 showed that

in nucleic acids play a key role in distinguishing the serum patients with lung cancer from that of healthy people.…”

Section: Resultsmentioning

confidence: 99%

Diagnosis of Lung Cancer by ATR-FTIR Spectroscopy and Chemometrics

Yang

Qian

et al. 2021

Front. Oncol.

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“…In all assays of the FTIR-derived spectra, overlapping peaks were deconvoluted and analyzed based on the second-order derivative spectra and pre-determined bell-type Gaussian peak fitting function using the OriginPro software (version 2021, OriginLab Corporation, Northampton, MA USA) (S4 Fig in S1 File ) [ 49 , 50 ].…”

Section: Methodsmentioning

confidence: 99%

Mechanisms of reducing joint stiffness by blocking collagen fibrillogenesis in a rabbit model of posttraumatic arthrofibrosis

et al. 2021

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Posttraumatic fibrotic scarring is a significant medical problem that alters the proper functioning of injured tissues. Current methods to reduce posttraumatic fibrosis rely on anti-inflammatory and anti-proliferative agents with broad intracellular targets. As a result, their use is not fully effective and may cause unwanted side effects. Our group previously demonstrated that extracellular collagen fibrillogenesis is a valid and specific target to reduce collagen-rich scar buildup. Our previous studies showed that a rationally designed antibody that binds the C-terminal telopeptide of the α2(I) chain involved in the aggregation of collagen molecules limits fibril assembly in vitro and reduces scar formation in vivo. Here, we have utilized a clinically relevant arthrofibrosis model to study the broad mechanisms of the anti-scarring activity of this antibody. Moreover, we analyzed the effects of targeting collagen fibril formation on the quality of healed joint tissues, including the posterior capsule, patellar tendon, and subchondral bone. Our results show that blocking collagen fibrillogenesis not only reduces collagen content in the scar, but also accelerates the remodeling of healing tissues and changes the collagen fibrils’ cross-linking. In total, this study demonstrated that targeting collagen fibrillogenesis to limit arthrofibrosis affects neither the quality of healing of the joint tissues nor disturbs vital tissues and organs.

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“…The synthesized nHA compound was subjected to FTIR as shown in Figure 3a. The presence of peaks at 1092 (PO 4 3− , ν 1 ), 1023 (PO 4 3− , ν 1 ), 963 (OH −1 ), 601 (HPO 4 2− ), and 561 cm −1 (PO 4 3− , ν 3 ) were the characteristic bands for nHA confirming its successful synthesis [35,36]. A very small peak of OH was visible at 3571 cm −1 affirming that the amount of H 2 O was very low.…”

Section: Ftir Analysismentioning

confidence: 74%

Comparative Study of Crystallization, Mechanical Properties, and In Vitro Cytotoxicity of Nanocomposites at Low Filler Loadings of Hydroxyapatite for Bone-Tissue Engineering Based on Poly(l-lactic acid)/Cyclo Olefin Copolymer

Nazir

Iqbal

2021

Polymers

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A poly(l-lactic acid)/nanohydroxyapatite (PLLA/nHA) scaffold works as a bioactive, osteoconductive scaffold for bone-tissue engineering, but its low degradation rate limits embedded HA in PLLA to efficiently interact with body fluids. In this work, nano-hydroxyapatite (nHA) was added in lower filler loadings (1, 5, 10, and 20 wt%) in a poly(l-lactic acid)/cyclo olefin copolymer10 wt% (PLLA/COC10) blend to obtain novel poly(l-lactic acid)/cyclo olefin copolymer/nanohydroxyapatite (PLLA/COC10-nHA) scaffolds for bone-tissue regeneration and repair. Furthermore, the structure-activity relationship of PLLA/COC10-nHA (ternary system) nanocomposites in comparison with PLLA/nHA (binary system) nanocomposites was systematically studied. Nanocomposites were evaluated for structural (morphology, crystallization), thermomechanical properties, antibacterial potential, and cytocompatibility for bone-tissue engineering applications. Scanning electron microscope images revealed that PLLA/COC10-nHA had uniform morphology and dispersion of nanoparticles up to 10% of HA, and the overall nHA dispersion in matrix was better in PLLA/COC10-nHA as compared to PLLA/nHA. Fourier transformation infrared spectroscopy (FTIR), powder X-ray diffraction (XRD), and differential scanning calorimetry (DSC) studies confirmed miscibility and transformation of the α-crystal form of PLLA to the ά-crystal form by the addition of nHA in all nanocomposites. The degree of crystallinity (%) in the case of PLLA/COC10-nHA 10 wt% was 114% higher than pure PLLA/COC10 and 128% higher than pristine PLLA, indicating COC and nHA are acting as nucleating agents in the PLLA/COC10-nHA nanocomposites, causing an increase in the degree of crystallinity (%). Moreover, PLLA/COC10-nHA exhibited 140 to 240% (1–20 wt% HA) enhanced mechanical properties in terms of ductility as compared to PLLA/nHA. Antibacterial activity results showed that 10 wt% HA in PLLA/COC10-nHA showed substantial activity against P. aeruginosa, S. aureus, and L. monocytogenes. In vitro cytocompatibility of PLLA/COC10 and PLLA nanocomposites with nHA osteoprogenitor cells (MC3T3-E1) and bone mesenchymal stem cells (BMSC) was evaluated. Both cell lines showed two- to three-fold enhancement in cell viability and 10- to 30-fold in proliferation upon culture on PLLA/COC10-nHA as compared to PLLA/nHA composites. It was observed that the ternary system PLLA/COC10-nHA had good dispersion and interfacial interaction resulting in improved thermomechanical and enhanced osteoconductive properties as compared to PLLA/nHA.

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Collagen maturity and mineralization in mesenchymal stem cells cultured on the hydroxyapatite-based bone scaffold analyzed by ATR-FTIR spectroscopic imaging

Cited by 34 publications

References 59 publications

Diagnosis of Lung Cancer by ATR-FTIR Spectroscopy and Chemometrics

Diagnosis of Lung Cancer by ATR-FTIR Spectroscopy and Chemometrics

Mechanisms of reducing joint stiffness by blocking collagen fibrillogenesis in a rabbit model of posttraumatic arthrofibrosis

Comparative Study of Crystallization, Mechanical Properties, and In Vitro Cytotoxicity of Nanocomposites at Low Filler Loadings of Hydroxyapatite for Bone-Tissue Engineering Based on Poly(l-lactic acid)/Cyclo Olefin Copolymer

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