Collagen matrix influences the morphologic features and steroid secretion of human granulosa cells

Ben‐Rafael, Zion; Benadiva, Claudio; Mastroianni, Luigi; García, Carmen Juan; Minda, Justina M.; Iozzo, Renato V.; Flickinger, George L.

doi:10.1016/0002-9378(88)90596-0

Cited by 30 publications

(26 citation statements)

References 16 publications

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“…In our model, we can suggest that the drastic cell rounding triggered by addition of the anti-6 IgG directly affected GC steroidogenesis. Accordingly, previous studies have also reported an enhancement of E2 secretion by bovine or human GC exhibiting a rounded rather than a flattened shape (Ben-Rafael et al 1988, Bussenot et al 1993, Gutiérrez et al 1997. However, in rat (Carnegie & Tsang 1988), human (Ben-Rafael et al 1988) and pig (Sites et al 1996), in contrast to sheep, a rounded cell shape has also been associated with an enhancement of P4 secretion by GC.…”

Section: Discussionmentioning

confidence: 91%

“…Accordingly, previous studies have also reported an enhancement of E2 secretion by bovine or human GC exhibiting a rounded rather than a flattened shape (Ben-Rafael et al 1988, Bussenot et al 1993, Gutiérrez et al 1997. However, in rat (Carnegie & Tsang 1988), human (Ben-Rafael et al 1988) and pig (Sites et al 1996), in contrast to sheep, a rounded cell shape has also been associated with an enhancement of P4 secretion by GC. Alternatively, one can hypothesize that activation of the integrin 6 1 by LN would have promoted activation of signaling pathways modulating E2 and P4 secretion differently, independently of modulation of cell shape.…”

Section: Discussionmentioning

confidence: 91%

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Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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This study aimed to determine the physiological role of laminin (LN) and its receptor, 6 1 integrin, in controlling the functions of granulosa cells (GC) during follicular development in sheep ovary. Immunohistochemistry experiments showed the presence of increasing levels of LN (P<0·0001), and high levels of mature 6 1 integrin in GC layers of healthy antral follicles during the follicular and the preovulatory phases of the estrous cycle. In vitro, the addition of a function-blocking antibody raised against 6 subunit (anti-6 IgG) to the medium of ovine GC cultured on LN impaired cell spreading (P<0·0001), decreased the proliferation rate (P<0·05) and increased the apoptosis rate (P<0·05). Furthermore, addition of anti-6 IgG enhanced estradiol (E2) secretion by GC in the presence or absence of follicle-stimulating hormone (FSH), luteinizing hormone or insulin-like growth factor-I in culture medium (P<0·0001), and inhibited progesterone (P4) secretion in basal conditions or in the presence of low (0·5 ng/ml) FSH concentrations only (P<0·0001). The anti-6 IgG effect was specific to an interaction of LN with 6 1 integrin since it was ineffective on GC cultured on heat-denatured LN, RGD (arginine-glycine-aspartic acid) peptides and non-coated substratum. Hence, this study established that 6 1 integrin 1) was expressed in GC of antral follicles, 2) mediated the actions of LN on survival, proliferation and steroidogenesis of GC, and 3) was able to dramatically modulate P4 and E2 secretion by GC in vitro. It is suggested that during the follicular and the preovulatory phases of the estrous cycle, the increasing levels of LN in GC of large antral follicles might support their final development to ovulation.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 91%

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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“…27 Rat and human granulosa cells plated on bovine corneal epithelium ECM or type I collagen gel attached significantly faster compared with cells plated on tissue culture plastic (all cells were observed as round/ epithelioid in shape). [28][29][30] The interaction between the granulosa cell and ECM is highly regulated and specific, given that ovine granulosa cell binding to laminin could be reduced with antibodies specific to anti-α 6 integrin antibody. 31 In addition to the ECM identity, the density of ECM molecules can regulate granulosa cell adhesion.…”

Section: Cell Shape and Attachmentmentioning

confidence: 99%

“…16 In rat and human granulosa cells, progesterone levels have been shown to be increased in the presence of bovine corneal endothelial ECM, HR9 mouse endodermal ECM, fibronectin, laminin, and type I collagen, but decreased with Matrigel. 21,28,29,34,39,41,46 In contrast, human granulosa cells plated on laminin have decreased progesterone levels compared with uncoated plates. 56 Type I collagen coating, type I collagen gels, poly-hema modified plates, and heparincoated plates affect the production of progesterone negatively in ovine granulosa cells isolated from large follicles (4 to 7 mm).…”

Section: Regulation Of Progesteronementioning

confidence: 99%

Ambulante Nachsorge aus gesundheitsökonomischer Sicht

Brandes¹,

Mau²,

Beck³

et al. 2006

Gesundheitswesen

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The extracellular matrix (ECM) promotes and/or inhibits many cellular processes, including but not limited to proliferation, differentiation, and survival, which must occur for follicle growth and oocyte maturation. The ECM regulation of cellular processes in ovarian cells is being investigated in many animal models, including avian, rat, bovine, porcine, rabbit, sheep, human, and mouse. Granulosa cells are more frequently employed; however, the culture of intact follicles and ovaries has been developed and enables ECM functions in folliculogenesis to be studied. ECMcomponents that have been examined are used individually (collagen, laminin, fibronectin) or collectively (Matrigel, isolated basal lamina, and ECM produced by cell lines) in both two-and three-dimensional model systems. In granulosa cell cultures, ECM affects morphology, aggregation and communication, survival, proliferation, and steroidogenesis; whereas follicle and ovary cultures demonstrate a regulation of folliculogenesis. This article describes the ECM functionality on ovarian cells throughout development, and highlights the potential of developing technologies to identify structure-function relationships in follicle maturation. KeywordsExtracellular matrix; granulosa cells; morphology; proliferation; survival Ovarian follicle development is regulated by endocrine, paracrine, and autocrine factors in a spatially and temporally regulated manner, and is characterized by dramatic changes throughout maturation. The ovary contains a large reserve of inactive primordial follicles arrested in prophase I, with a small cohort activated daily. Prior to activation, these immature follicles contain nongrowing oocytes and nondividing, flattened (squamous) pregranulosa cells surrounded by a basal lamina. Following activation, follicle growth begins with the transformation of squamous pregranulosa cells into a single layer of cuboidal granulosa cells surrounding a growing oocyte. Granulosa cells proliferate to form multiple layers, requiring expansion of the basal lamina, and leading to two-layered and multilayered follicle stages. At this time, an outer layer of cells, termed the theca layer, is recruited (or differentiated from existing stroma 1 ) and surrounds the basal lamina. A fluid-filled antral cavity, or antrum, forms in the granulosa cell compartment of the follicle later in folliculogenesis. Small antral follicles develop into mature preovulatory follicles in the presence of follicle-stimulating hormone

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“…Base-ment membrane, a thin ECM, is continuous in intact follicles whereas in luteinized follicles, it becomes diffuse and fragmented, which suggests an increased exposure of basement membrane components to luteal cells [5]. To understand the effects of the ECM on granulosa cells, collagen [6,7], laminin [8][9][10], fibronectin [10], and ECM produced by bovine corneal endothelial cells [11][12][13] have been examined as culture substrates.…”

mentioning

confidence: 99%

Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture.

Hwang

Kee

Kim³

et al. 2000

Endocr J

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Abstract.The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3~-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization.

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Collagen matrix influences the morphologic features and steroid secretion of human granulosa cells

Cited by 30 publications

References 16 publications

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Ambulante Nachsorge aus gesundheitsökonomischer Sicht

Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture.

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