Collagen-IV and laminin-1 regulate estrogen receptor α expression and function in mouse mammary epithelial cells

Novaro, Virginia; Roskelley, Calvin D.; Bissell, Mina J.

doi:10.1242/jcs.00523

Cited by 83 publications

(65 citation statements)

References 54 publications

(57 reference statements)

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“…The following primers were used to amplify ␤-casein, ␥-casein, lactoferrin, and GAPDH cDNA sequences: forward primer of the ␤-casein gene 5Ј-GCT CAG GCT CAA ACC ATC TC-3Ј and reverse primer 5Ј-TGT GGA AGG AAG GGT GCT AC-3Ј; forward primer of the ␥-casein gene: 5Ј-CCC AGG AGT CTT CCT TTT CC-3Ј and reverse primer 5Ј-GGA AAC CAC GAA GAA ACC AA-3Ј; forward primer of the lactoferrin gene: 5Ј-AGT GAG GAG AAG CGC AAG TGT G-3Ј and reverse primer 5Ј-AGC CCC AGT GTA GCC TTG GTA T-3Ј; and forward primer for GAPDH gene 5ЈCCC CTG GCC AAG GTC ATC CAT GAC-3Ј and reverse primer 5ЈCAT ACC AGG AAA TGA GCT TGA CAA AG-3Ј. Quantitative real time PCR analysis was performed with the Lightcycler System (Roche Applied Science) using the Lightcycler FastStart DNA Master SYBR Green I kit (Roche Applied Science) (31). The following Lightcycler PCR amplification protocol was used: 95°C for 10 min (initial denaturation) and 45 amplification cycles (95°C for 5 s, 60°C for 10 s, and 72°C for 5 s).…”

Section: Methodsmentioning

confidence: 99%

Extracellular Matrix-regulated Gene Expression Requires Cooperation of SWI/SNF and Transcription Factors

Spencer

Bissell

2007

Journal of Biological Chemistry

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Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation assays and mammary-specific genes as models, we show here that extracellular matrix molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the ␤-and ␥-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/ SNF complex, significantly reduced both ␤-and ␥-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. Chromatin immunoprecipitation analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Co-immunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, CCAAT/enhancer-binding protein ␤, and glucocorticoid receptor. Thus, extracellular matrix-and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.Differentiated function of mammary epithelial cells is regulated by signals from both ECM 2 and lactogenic hormones (1-3). The gene encoding the milk protein, ␤-casein, has been used widely as a marker for functional differentiation of MECs. We and others have shown that in both primary mouse mammary epithelial cells and immortalized mammary epithelial cell lines (4 -6), transcription of ␤-casein requires signals from both laminin-111 (previously referred to as laminin-1) and prolactin (1, 2, 7-10). A number of transcription factors, including STAT5, C/EBP␤, and GR, have been shown to be involved in this process (reviewed in Ref. 7).Modulation of chromatin structure by histone modifications and ATP-dependent remodeling has been implicated in cell differentiation and transcriptional control of tissue-specific and inducible genes (11-13). Histone-modifying enzymes are believed to be recruited to promoter regions through their association with transcription factors and are critical for tissue-specific gene expression and functional differentiation of specific cell types (14, 15). Histone acetylation is a dynamic process and is regulated by histone acetyltransferases and histone deacetylases (16). The mapping of global histone acetylation patterns has demonstrated that chromatin accessibility and gene expression are correlated with histone hyperacetylation of promoters and other cis-elements (17, 18). The p300 histone acetyltransferase cooperates with STAT5 to enhance exogenous ␤-casein promoter activity in COS cells, indicating that histone acetylation may play a role in ␤-casein transcription (19). Trichostatin A (TSA), an inhibitor of histone deacetylase, was shown to activate the bovine casein ECM response element (BCE-1) in an ECM-independent...

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Section: Methodsmentioning

confidence: 99%

Extracellular Matrix-regulated Gene Expression Requires Cooperation of SWI/SNF and Transcription Factors

Spencer

Bissell

2007

Journal of Biological Chemistry

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“…Alternatively, in vitro work has suggested that ECM can alter expression subtype markers, further confounding the link to prognosis [65,66] As a result, studies with different clinical samples can show very different results, and caution is needed in interpreting findings.…”

Section: Breast Cancer Subtypesmentioning

confidence: 99%

The extracellular matrix in breast cancer predicts prognosis through composition, splicing, and crosslinking

Robertson

2016

Experimental Cell Research

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The extracellular matrix in breast cancer predicts prognosis through composition, splicing, and crosslinking a b s t r a c tThe extracellular matrix in the healthy breast has an important tumor suppressive role, whereas the abnormal ECM in tumors can promote aggressiveness, and has been linked to breast cancer relapse, survival and resistance to chemotherapy. This review article gives an overview of the elements of the ECM which have been linked to prognosis of breast cancers, including changes in ECM protein composition, splicing, and microstructure. Published by Elsevier Inc.

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“…13 Although these cells do not secrete basement membrane components collagen IV 17 or laminin V (our own observations), they do form organized structures in 3D culture. 13,17,18 The rationale for using T47D breast cancer cells is based on the following considerations: (i) the fact that there are no established normal human breast cell lines that are estrogen sensitive regarding the control of cell proliferation, 19 (ii) that cells in primary cultures rapidly lose ER expression, 20 and (iii) that of all established estrogensensitive cell lines, T47D cells seem to form the most normallike structures among those tested. 13,21 Here we examine the effect of the mammotrophic hormones 17 b-estradiol (E2), the progestagen promegestone, and prolactin in a 3D culture model and conclude that overall, cells in this model respond to these hormones in a comparable fashion to that which occurs in vivo with respect to cell proliferation, morphology, and matrix organization.…”

Section: Introductionmentioning

confidence: 99%

Hormonal Regulation of Epithelial Organization in a Three-Dimensional Breast Tissue Culture Model

Speroni

Whitt

Xylas

et al. 2014

Tissue Engineering Part C: Methods

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The establishment of hormone target breast cells in the 1970's resulted in suitable models for the study of hormone control of cell proliferation and gene expression using two-dimensional (2D) cultures. However, to study mammogenesis and breast tumor development in vitro, cells must be able to organize in three-dimensional (3D) structures like in the tissue. We now report the development of a hormone-sensitive 3D culture model for the study of mammogenesis and neoplastic development. Hormone-sensitive T47D breast cancer cells respond to estradiol in a dose-dependent manner by forming complex epithelial structures. Treatment with the synthetic progestagen promegestone, in the presence of estradiol, results in flat epithelial structures that display cytoplasmic projections, a phenomenon reported to precede side-branching. Additionally, as in the mammary gland, treatment with prolactin in the presence of estradiol induces budding structures. These changes in epithelial organization are accompanied by collagen remodeling. Collagen is the major acellular component of the breast stroma and an important player in tumor development and progression. Quantitative analysis of second harmonic generation of collagen fibers revealed that collagen density was more variable surrounding budding and irregularly shaped structures when compared to more regular structures; suggesting that fiber organization in the former is more anisotropic than in the latter. In sum, this new 3D model recapitulates morphogenetic events modulated by mammogenic hormones in the breast, and is suitable for the evaluation of therapeutic agents.

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Collagen-IV and laminin-1 regulate estrogen receptor α expression and function in mouse mammary epithelial cells

Cited by 83 publications

References 54 publications

Extracellular Matrix-regulated Gene Expression Requires Cooperation of SWI/SNF and Transcription Factors

Extracellular Matrix-regulated Gene Expression Requires Cooperation of SWI/SNF and Transcription Factors

The extracellular matrix in breast cancer predicts prognosis through composition, splicing, and crosslinking

Hormonal Regulation of Epithelial Organization in a Three-Dimensional Breast Tissue Culture Model

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