Collagen-induced Arthritis: A Model for Murine Autoimmune Arthritis

Pietrosimone, Kathryn; Jin, Mengyao; Poston, Brad; Liu, Peng

doi:10.21769/bioprotoc.1626

Cited by 71 publications

(64 citation statements)

References 3 publications

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“…The mice were divided into three groups: control (treated with saline), CIA, and CIA + HDL. The CIA and CIA + HDL groups were induced according to a previous report (10). On day 0, DBA/1 mice were immunized with bovine type II collagen in an emulsion with FCA.…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory Effects of HDL in Mice With Rheumatoid Arthritis Induced by Collagen

Wang

Zhang

et al. 2018

Front. Immunol.

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ObjectiveTo investigate the anti-inflammatory effects of high-density lipoprotein (HDL) in mice with rheumatoid arthritis (RA) induced by collagen.MethodsMale DBA/1 mice (8-week-old) were divided into three groups: control (treated with saline), collagen-induced arthritis (CIA), and CIA + HDL. CIA was induced with bovine type II collagen, and after the injection of bovine type II collagen, the CIA + HDL group received an injection of HDL on day 28 followed by HDL injections four times every 3 days. Mice were weighed, the paws were scored, and paw thickness was measured beginning on day 21. Additionally, the levels of tumor necrosis factor-alpha (TNF-α) and IL-6 were measured by ELISA kits, tissue sections of paws were stained with hematoxylin and eosin, and the inflammatory signaling pathway was analyzed by western blotting.ResultsWe found that the production of pro-inflammatory cytokines TNF-α and IL-6 in mice which received HDL decreased 45.14 and 35.02%, respectively. And we also found that HDL could significantly decrease the level of anti-type-II-collagen IgG2a and inhibit the neutrophil infiltration and cell proliferation and protect the ankle joint from type II collage-induced injury. Western blot analysis indicated that HDL could also inhibit the activation of the NF-κB, MAPK, and ERK signaling pathways in RA mice.ConclusionHDL can inhibit the inflammation induced by bovine type II collagen and the development of RA.

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Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory Effects of HDL in Mice With Rheumatoid Arthritis Induced by Collagen

Wang

Zhang

et al. 2018

Front. Immunol.

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“…The most widely used model for studying the complete disease process is the collagen-immunization model [51]. This model includes both the priming or initiation phase of the disease, which requires T- and B-cell responses leading to production of pathogenic anti-collagen and other antibodies, as well as the effector phases, which involve recruitment of neutrophils to the inflamed joint, leading to cellular activation with cartilage and bone injury.…”

Section: Roles For Neutrophils In Classical Autoimmune Disease Modelsmentioning

confidence: 99%

Neutrophils in animal models of autoimmune disease

Németh

Mócsai

Lowell

2016

Seminars in Immunology

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Neutrophils have traditionally been thought to play only a peripheral role in the genesis of many autoimmune and inflammatory diseases. However, recent studies in a variety of animal models suggest that these cells are central to the initiation and propagation of autoimmunity. The use of mouse models, which allow either deletion of neutrophils or the targeting of specific neutrophil functions, has revealed the many complex ways these cells contribute to autoimmune/inflammatory processes. This includes generation of self antigens through the process of NETosis, regulation of T-cell and dendritic cell activation, production of cytokines such as BAFF that stimulate self-reactive B-cells, as well as indirect effects on epithelial cell stability. In comparing the many different autoimmune models in which neutrophils have been examined, a number of common underlying themes emerge – such as a role for neutrophils in stimulating vascular permeability in arthritis, encephalitis and colitis. The use of animal models has also stimulated the development of new therapeutics that target neutrophil functions, such as NETosis, that may prove beneficial in human disease. This review will summarize neutrophil contributions in a number of murine autoimmune/inflammatory disease models.

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“…CIA is induced in mice with articular bovine type II collagen (CII) and the resulting disease is essentially mediated by an autoimmune response. CIA is commonly used to study in vivo the pathological mechanisms of RA and to evaluate new antiarthritic drugs as it shares clinical, histological, and immunological features of human RA [9,10]. e sublingual administration is the mode of administration of MIM, including for the tested MIM.…”

Section: Introductionmentioning

confidence: 99%

The Micro-Immunotherapy Medicine 2LARTH® Reduces Inflammation and Symptoms of Rheumatoid Arthritis In Vivo

Floris¹,

Palomares

Appel³

et al. 2020

International Journal of Rheumatology

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Background. Rheumatoid arthritis (RA) is a chronic inflammatory joint disease, which can cause cartilage and bone damages as well as pain and disability. In order to prevent disease progression, reduce pain, and major symptoms of RA, one good strategy consists in targeting proinflammatory cytokines that have the key role in the vicious circle of synovial inflammation and pain. The micro-immunotherapy medicine (MIM) 2LARTH® targets cytokines involved in inflammation. Aim. The aim of the study is to evaluate the effect of the MIM compared to vehicle in an in vivo model of RA, induced in mice after immunization with articular bovine type II collagen. Methods. Vehicle and MIM were dissolved in pure water (1 capsule in 100 ml) and 100 µl was given by gavage daily for 14 days. To evaluate the severity of arthritis, wrist and ankle thickness was determined, paw edema was measured, and a clinical score from 0 to 4 was established. Furthermore, histological analysis was performed. To evaluate systemic inflammation, circulating levels of IL-1β and TNF-α were measured by ELISA. Results. Ankle thickness was found to be significantly reduced in MIM-treated mice compared to vehicle-treated mice (P<0.05) and compared to untreated me (P<0.01). Paw edema was reduced, as well as clinical score attributed to MIM-treated mice in comparison with vehicle-treated mice and untreated CIA mice (P<0.01). In line with these results, histological analysis confirmed that MIM reduced inflammation and joint destruction in comparison to controls. No significant changes were found in plasmatic IL-1β levels between CIA and controls, while the levels of TNF-α significantly increased in the CIA group, and were lowered in MIM-treated mice (P<0.05 vs. vehicle and vs. CIA). Conclusion. The results indicate that the tested medicine reduces inflammation, histological, and clinical signs of RA in a CIA model.

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Collagen-induced Arthritis: A Model for Murine Autoimmune Arthritis

Cited by 71 publications

References 3 publications

Anti-Inflammatory Effects of HDL in Mice With Rheumatoid Arthritis Induced by Collagen

Anti-Inflammatory Effects of HDL in Mice With Rheumatoid Arthritis Induced by Collagen

Neutrophils in animal models of autoimmune disease

The Micro-Immunotherapy Medicine 2LARTH® Reduces Inflammation and Symptoms of Rheumatoid Arthritis In Vivo

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