Collagen-induced arthritis

Abstract

The collagen-induced arthritis (CIA) mouse model is the most commonly studied autoimmune model of rheumatoid arthritis. Autoimmune arthritis is induced in this model by immunization with an emulsion of complete Freund's adjuvant and type II collagen (CII). This protocol describes the steps necessary for acquisition, handling and preparation of CII, as well as selection of mouse strains, proper immunization technique and evaluation of the arthritis incidence and severity. Typically, the first signs of arthritis appear in this model 21-28 days after immunization, and identification of the arthritic limbs is not difficult. Using the protocol described, the investigator should be able to reproducibly induce a high incidence of CIA in various strains of genetically susceptible mice as well as learn how to critically evaluate the pathology of the disease. The total time for the preparation of reagents and the immunization of ten mice is about 1.5 h.

Pharmacological inhibition of Dock5 prevents osteolysis by affecting osteoclast podosome organization while preserving bone formation

“…It can be modelled by immunization of susceptible DBA/ 1 mouse strain with type II collagen, the major protein component of articular cartilage 18 (Fig. 3a).…”

Olfactory ecto-mesenchymal stem cells possess immunoregulatory function and suppress autoimmune arthritis

“…Joint tissues were sectioned at 4 mm thickness and stained with hematoxylin and eosin for histopathological evaluation. 19 Co-culture of OE-MSCs and T cells OE-MSCs were co-cultured with splenic CD4 1 T cells that were sorted from wild-type mice using CD4 1 T-cell microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Responder cells were co-cultured with different OE-MSC ratios in U-bottomed 96-well plates (Costar, Corning, NY) in the presence of anti-CD3 (10 mg mL 21 , Biolegend, San Diego, CA) and anti-CD28 (5 mg mL 21 , Biolegend, San Diego, CA) monoclonal antibodies (mAbs) for 72 h, and they were pulsed with [ 3 H]-thymidine (Pharmacia, Stockholm, Sweden, 1 mCi/well) for the last 16 h of culture.…”

Rapid Response Fluorescence Probe Enabled In Vivo Diagnosis and Assessing Treatment Response of Hypochlorous Acid‐Mediated Rheumatoid Arthritis

“…This disease affects ≈25 million people, ≈1% of adults in the world population 2. It is reported that genetic and environmental factors maybe the cause for RA, but the etiology for this inflammatory disease is not fully elucidated 3. For the treatment of RA, low dose of methotrexate (MTX) has long been recognized as a standard for patients suffering from this disease,4 yet the mechanism involved in its activity against RA remains less clear 1, 5.…”