Collagen implants remain supple not allowing fibroblast ingrowth

Boon, Mathilde E.; Ruijgrok, J.M.; Vardaxis, M.J.

doi:10.1016/0142-9612(95)98905-t

Cited by 20 publications

(14 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is not yet known whether in the in vivo setting SM cells will invade Permacol. However, a study by Boon et al [17] showed that fibroblasts were unable to penetrate cross-linked collagen implanted subcutaneously into rats. Although the body is a far better incubator than any in vitro system, the present data suggest that SM cells would be inhibited from migrating from the remnant bladder into Permacol to form a new SM cell layer.…”

Section: Fig 3 Hande Stained (A-d) and Immunoperoxidase-labelled (Efmentioning

confidence: 99%

In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction

2004

View full text Add to dashboard Cite

The contribution by the units in York and Leeds to tissue‐engineering research is considerable, and they describe here their work to assess a natural matrix for use in the urinary tract, and to develop an in vitro regimen for assessing the biocompatibility of potential biomaterials. They describe how they have developed a simple, reproducible and rigorous regimen which will help to identify the causes of potential bio‐incompatibility. Buccal mucosa has become the tissue of choice for urethroplasty. Authors from Edinburgh report on the oral complications after its harvesting; they found that most patients were satisfied with its use, but that it had long‐term complications, about which patients should be informed. OBJECTIVES To assess the potential of PermacolTM (Tissue Science Laboratories, Swillington, UK), a natural matrix derived from decellularized porcine dermis, as a matrix for urological tissue engineering, and thus to develop an in vitro regimen for assessing the biocompatibility of potential biomaterials before experimentation in animal models. MATERIALS AND METHODS Urinary tract‐derived normal human urothelial (NHU) and smooth muscle (SM) cells were grown in monoculture as autologous cell lines. Permacol was assessed for its ability to support colonization by NHU and SM cells. The failure of the Permacol matrix to be infiltrated by SM cells was further investigated using the highly invasive EJ bladder cancer cell line. RESULTS NHU cells readily attached and grew as a monolayer on the surface of Permacol. Cells stratified when the culture medium was supplemented with 2 mmol/L calcium. EJ cells initially grew on the surface and subsequently invaded the matrix, while SM cells only colonized the surface of Permacol when cocultured with NHU cells. Cytoxicity, evaluated by contact inhibition and conditioned‐medium assays, excluded the presence of soluble toxins in the biomaterial. CONCLUSIONS We developed a simple, reproducible and rigorous regimen for assessing potential biomaterials in vitro. Applying this system might reduce the use of animals and help to identify causes of potential bio‐incompatibility. The inability of SM cells to penetrate the Permacol matrix suggests that required matrix‐bound signalling factors are absent, possibly as a result of the procedures used for processing Permacol. Identifying the key regulatory factors that regulate SM cell growth and orchestrate regenerative processes in the urinary tract will be important for developing suitable biomaterials for the bladder.

show abstract

Section: Fig 3 Hande Stained (A-d) and Immunoperoxidase-labelled (Efmentioning

confidence: 99%

In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction

2004

View full text Add to dashboard Cite

show abstract

“…Therefore, the extent of cross-linking of the material affects the rate of degradation. Varying degradation rates of glutaraldehydecross-linked collagen implants have been reported, ranging from a few weeks [62,63] to several months [64]. In the current study, a loss of the spherical structure of the collagen spheres was observed as early as 4 weeks following immersion in PBS at 37°C.…”

Section: Discussionmentioning

confidence: 64%

Microporous collagen spheres produced via thermally induced phase separation for tissue regeneration

Keshaw

Thapar²,

Burns³

et al. 2010

Acta Biomaterialia

View full text Add to dashboard Cite

“…This new methodology of reticulation was probably responsible for the reduced inflammatory reaction in our study. The use of 0.05% GA as the cross-linking reagent, to control the biodegradability of the collagen membranes, was based on the fact that it has been the reagent of choice to improve the mechanical properties and biocompatibility of many implantable collagen devices [38]. Anionic collagen membranes cross-linked with 0.05% GA displayed good biocompatibility with no adverse biological effects and controlled biodegradation was achieved as a function of the time of GA exposure.…”

Section: Discussionmentioning

confidence: 99%

Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation

et al. 2010

View full text Add to dashboard Cite

Collagen implants remain supple not allowing fibroblast ingrowth

Cited by 20 publications

References 10 publications

In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction

In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction

Microporous collagen spheres produced via thermally induced phase separation for tissue regeneration

Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation

Contact Info

Product

Resources

About