Collagen I Promotes Adipocytogenesis in Adipose-Derived Stem Cells In Vitro

Abstract

A hallmark of ageing is the redistribution of body fat. Particularly, subcutaneous fat decreases paralleled by a decrease of skin collagen I are typical for age-related skin atrophy. In this paper, we hypothesize that collagen I may be a relevant molecule stimulating the differentiation of adipose-derived stem cells (ASCs) into adipocytes augmenting subcutaneous fat. In this context lipogenesis, adiponectin, and collagen I receptor expression were determined. Freshly isolated ASCs were characterized by stemness-associated surface markers by FACS analysis and then transdifferentiated into adipocytes by specific medium supplements. Lipogenesis was evaluated using Nile Red staining and documented by fluorescence microscopy or quantitatively measured by using a multiwell spectrofluorometer. Expression of adiponectin was measured by real-time RT-PCR and in cell-free supernatants by ELISA, and expression of collagen I receptors was observed by western blot analysis. It was found that supports coated with collagen I promote cell adhesion and lipogenesis of ASCs. Interestingly, a reverse correlation to adiponectin expression was observed. Moreover, we found upregulation of the collagen receptor, discoidin domain-containing receptor 2; receptors of the integrin family were absent or downregulated. These findings indicate that collagen I is able to modulate lipogenesis and adiponectin expression and therefore may contribute to metabolic dysfunctions associated with ageing.

Adipose Tissue Transplant Impregnated with Collagen Increases Engraftment by Promoting Cell Proliferation, Neovascularization, and Macrophage Activity in a Rat

“…Furthermore, it has been reported that hypoxia results in the ASC secretion of neovascularization growth factors such as VEGF, and therefore induces neovascularization in an ischemic environment after transplantation [33]. ASC presents in subcutaneous adipose tissue can differentiate into adipocytes [34]; it has been reported that when ASC are cultured from collagen, more lipids are produced, which is a marker of adipose differentiation [35]. Therefore, collagen impregnation could support ASC differentiation into adipocytes.…”

Modeling Adipogenesis: Current and Future Perspective

“…To address how ECM affects adipogenesis and adipocyte function, Type I collagen-based hydrogel can be utilized to mimic physiologically relevant adipogenic microenvironments, since it can be modified in terms of biophysical and biochemical properties [ 112 ]. Tissue culture plastic coated with type I collagen has been reported to enhance adipogenesis [ 113 ], while type VI collagen appears to restrict adipose expansion [ 114 ]. Interestingly, by blocking an attachment of integrin alpha 6 to laminin, it allowed differentiation of adipocytes due to sustained anti-adipogenic RhoA activity [ 115 ].…”

Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation

“…Biomaterials should provide an adequate environment for cultures cells and thereby play a crucial role in tissue engineering process. To date there are a variety of available materials for adipose tissue engineering such as gelatine [1], collagen [2], alginate [3] or combinations thereof. However, these materials cannot represent the complexity of the natural ECM and the typical organization of native tissue.…”