Collagen gel matrix assay as an in vitro chemosensitivity test for malignant astrocytic tumors

Ono, Atsushi; Kanno, Hiroshi; Hayashi, Akimune; Nishimura, Satoshi; Kyuma, Yoshikazu; Sato, Hidemitsu; Ito, Susumu; Shimizu, Nobuyuki; Chang, Chia‐Cheng; Gondo, Gakuji; Yamamoto, Isao; Sasaki, Takuma; Tanaka, Motohiro

doi:10.1007/s10147-006-0636-8

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Patients were treated with those cytostatics which exhibited a high growth inhibitory rate in 3-dimensional cultures. Individual chemotherapy based on in vitro chemosensitivity contributed to longer survival of the treated patients [14].…”

Section: Resultsmentioning

confidence: 99%

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

Zdzisińska

Roliński²,

Piersiak³

et al. 2009

Folia Histochem Cytobiol.

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Abstract:We examined cytokine production by bone marrow stromal cells (BMSCs) of patients with multiple myeloma (MM) in response to contact with myeloma RPMI8226 cells in standard 2-dimensional (2D) cultures and in 3-dimensional (3D) cultures on a gelatine sponge scaffold. It was detected that BMSCs in the 3D cultures produced more IL-11 and HGF and less IL-10 than in the 2D cultures. Moreover, RPMI8226 cells after contact with BMSCs in 3D cultures produced more sIL-6R than in the classic 2D cultures. We concluded that 3D cultures of BMSCs with myeloma cells offered a promising model for in vitro examination of interactions between myeloma cells and the bone marrow stroma and for examination of potent antimyeloma agents.

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Section: Resultsmentioning

confidence: 99%

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

Zdzisińska

Roliński²,

Piersiak³

et al. 2009

Folia Histochem Cytobiol.

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“…To better compare with clinical outcomes, anti-glioma inhibition rates were investigated according to drug PPCs of human blood in 2D and 3D culture. PPCs of the three tested drugs were TMZ: 258.0 μM, CCNU: 14.0 μM and DDP: 12.8 μM [ 48 – 50 ]. After 24 h, drug-containing medium was replaced with fresh medium and cells were incubated for 48 h. After treatment, chemosensitivity was determined using the CCK8 assay.…”

Section: Methodsmentioning

confidence: 99%

A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics

Ping

et al. 2016

Oncotarget

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Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics.

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“…Previous studies focused on maintaining human GBM tissue, date back to the 1970s, and using static culture techniques in culture dishes that required the culture media to be replenished every 1–4 days [[40], [41], [42], [43]]. More recently, a number of groups have maintained human GBM tissues on collagen gel matrices [44] and on membrane culture inserts (Millipore) [45,46] but none of these experimental models have applied continuous tissue flow. Compared with previous static culture experiments, the length of tissue culture in the present study is considerably shorter, with only 2 tissue sections cultured for more than 3 days, one for 5 days and another for a week.…”

Section: Discussionmentioning

confidence: 99%

“…Ono et al. cultured 22 GBM tissues on collagen gel matrix assays for 7 days and treated the tissues with a variety of chemotherapeutic agents, and although the group were able to assess the inhibitory effects of the drugs, they did not report on the viability of the untreated tissues [44]. Merz et al.…”

Section: Discussionmentioning

confidence: 99%

Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model?

Olubajo

Achawal

Greenman

2020

Translational Oncology

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BACKGROUND: One way to overcome the genetic and molecular variations within glioblastoma is to treat each tumour on an individual basis. To facilitate this, we have developed a microfluidic culture paradigm that maintains human glioblastoma tissue ex vivo. METHODS: The assembled device, fabricated using a photolithographic process, is composed of two layers of glass bonded together to contain a tissue chamber and a network of microchannels that allow continued tissue perfusion. RESULTS: A total of 128 tissue biopsies (from 33 patients) were maintained in microfluidic devices for an average of 72 hours. Tissue viability (measured with Annexin V and propidium iodide) was 61.1% in tissue maintained on chip compared with 68.9% for fresh tissue analysed at commencement of the experiments. Other biomarkers, including lactate dehydrogenase absorbance and trypan blue exclusion, supported the viability of the tissue maintained on chip. Histological appearances remained unchanged during the tissue maintenance period, and immunohistochemical analysis of Ki67 and caspase 3 showed no significant differences when compared with fresh tissues. A trend showed that tumours associated with poorer outcomes (recurrent tumours and Isocitrate Dehydrogenase - IDH wildtype) displayed higher viability on chip than tumours linked with improved outcomes (low-grade gliomas, IDH mutants and primary tumours). conclusions: This work has demonstrated for the first time that human glioblastoma tissue can be successfully maintained within a microfluidic device and has the potential to be developed as a new platform for studying the biology of brain tumours, with the long-term aim of replacing current preclinical GBM models and facilitating personalised treatments.

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Collagen gel matrix assay as an in vitro chemosensitivity test for malignant astrocytic tumors

Cited by 9 publications

References 10 publications

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics

Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model?

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