Collagen-functionalized electrospun smooth and porous polymeric scaffolds for the development of human skin-equivalent

Girija, Aswathy Ravindran; Palaninathan, Vivekanandan; Strudwick, Xanthe L.; Balasubramanian, Sivakumar; Nair, Sakthikumar Dasappan; Cowin, Allison J.

doi:10.1039/d0ra04648e

Cited by 21 publications

(18 citation statements)

References 38 publications

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“…When fibroblast cells were cultured on collagen-modified fibers, the cells exhibited spindle-like elongated patterns, whereas keratinocytes exhibited a uniform patch of cells with good cell–cell interactions. As discussed in our in vitro study [ 34 ], while the unmodified smooth scaffold displayed better cell adhesion than unmodified porous scaffolds, collagen-modified scaffolds (both porous and smooth) produced confluent and uniform epidermal sheets of keratinocytes on one plane, with healthy fibroblasts within the scaffolds. Collagen-modified electrospun smooth and porous PLLA scaffolds of 10 mm diameter and unmodified counterparts were applied to the mice on the day of injury; full-thickness excisional wounds beneath a Tegaderm dressing ( Figure 1 b) were used to assess their ability to improve wound healing.…”

Section: Resultsmentioning

confidence: 92%

“…PLLA scaffolds were fabricated by an electrospinner (Nanon- O1A MECC Co. Ltd. Fukuda, Japan) with PLLA, (Mw of 80,000–100,000, Polyscience Inc., Warrington, PA, USA) [ 34 ]. Smooth fibers were fabricated by dissolving PLLA in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) to obtain a 14.5% w / v of PLLA solution using magnetic stirring for 4 h; this was followed by 4 h rest at room temperature (RT).…”

Section: Methodsmentioning

confidence: 99%

“…For collagen-modified scaffolds, both UV-sterilized smooth and porous PLLA scaffolds were soaked in 0.1% collagen for 30 min prior to application. Cocultures of keratinocytes and fibroblasts upon the scaffolds were prepared as previously described [ 34 ], and the morphology of the original polymeric scaffolds (before and after collagen coating) and the adhered cells were examined after 5 days’ coculture by scanning electron microscopy (SEM) using a Zeiss Merlin FEG SEM following previously described methods [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…Collagen can be coated or grafted using different methods such as drop casting (adsorption) and cross linking, or by creating chemical bonding with activated carboxylic functional groups [ 31 , 32 , 33 ]. We have previously used a simple drop casting method to coat PLLA electrospun scaffolds with collagen, and have shown that the electrospun scaffolds with variation in surface topology and surface chemistry affected the cellular responses including adhesion, proliferation, differentiation, and migration of keratinocytes and fibroblasts, with collagen-modified scaffolds (both porous and smooth) producing confluent and uniform epidermal sheets of keratinocytes on one plane, with healthy fibroblasts populated within the scaffolds [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds

et al. 2023

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Background: Electrospun fibers are widely studied in regenerative medicine for their ability to mimic the extracellular matrix (ECM) and provide mechanical support. In vitro studies indicated that cell adhesion and migration is superior on smooth poly(L-lactic acid) (PLLA) electrospun scaffolds and porous scaffolds once biofunctionalized with collagen. Methods: The in vivo performance of PLLA scaffolds with modified topology and collagen biofunctionalization in full-thickness mouse wounds was assessed by cellular infiltration, wound closure and re-epithelialization and ECM deposition. Results: Early indications suggested unmodified, smooth PLLA scaffolds perform poorly, with limited cellular infiltration and matrix deposition around the scaffold, the largest wound area, a significantly larger panniculus gape, and lowest re-epithelialization; however, by day 14, no significant differences were observed. Collagen biofunctionalization may improve healing, as collagen-functionalized smooth scaffolds were smallest overall, and collagen-functionalized porous scaffolds were smaller than non-functionalized porous scaffolds; the highest re-epithelialization was observed in wounds treated with collagen-functionalized scaffolds. Conclusion: Our results suggest that limited incorporation of smooth PLLA scaffolds into the healing wound occurs, and that altering surface topology, particularly by utilizing collagen biofunctionalization, may improve healing. The differing performance of the unmodified scaffolds in the in vitro versus in vivo studies demonstrates the importance of preclinical testing.

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Section: Resultsmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds

et al. 2023

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“… Structure of PLLA-based scaffold designed for bone, cartilage, blood vessel, and skin regeneration. Reprinted with permission from Elsevier [ 82 ], Taylor & Francis [ 113 ], Dovepress [ 161 ], and RSC [ 162 ]. …”

Section: Figurementioning

confidence: 99%

Poly-l-Lactic Acid (PLLA)-Based Biomaterials for Regenerative Medicine: A Review on Processing and Applications

et al. 2022

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Synthetic biopolymers are effective cues to replace damaged tissue in the tissue engineering (TE) field, both for in vitro and in vivo application. Among them, poly-l-lactic acid (PLLA) has been highlighted as a biomaterial with tunable mechanical properties and biodegradability that allows for the fabrication of porous scaffolds with different micro/nanostructures via various approaches. In this review, we discuss the structure of PLLA, its main properties, and the most recent advances in overcoming its hydrophobic, synthetic nature, which limits biological signaling and protein absorption. With this aim, PLLA-based scaffolds can be exposed to surface modification or combined with other biomaterials, such as natural or synthetic polymers and bioceramics. Further, various fabrication technologies, such as phase separation, electrospinning, and 3D printing, of PLLA-based scaffolds are scrutinized along with the in vitro and in vivo applications employed in various tissue repair strategies. Overall, this review focuses on the properties and applications of PLLA in the TE field, finally affording an insight into future directions and challenges to address an effective improvement of scaffold properties.

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Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling

Gonçalves¹,

Leal²,

Moreira³

et al. 2023

Advanced NanoBiomed Research

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Herein, intestinal, skin, and pulmonary in vitro tissue models based on electrospun membranes of poly(ε‐caprolactone) (PCL) and cellulose acetate (CA), cellulose acetate phthalate (CAP), ethylcellulose (EC), or methylcellulose (MC) are presented. Physicochemical characterization and biocompatibility analyses of the scaffolds are carried out using colorectal adenocarcinoma cells (intestine), keratinocytes and fibroblasts (skin), and bronchial and alveolar epithelial cells (lung). PCL, PCL:CA, and PCL:EC are composed of nanofibers, whereas PCL:CAP and PCL:MC scaffolds comprise a combination of micro‐ and nanofibers. PCL, PCL:CA, PCL:CAP, and PCL:EC samples demonstrate water contact angles greater than 90° and are, therefore, hydrophobic, while PCL:MC mats display a hydrophilic behavior. In intestinal models, cells adhere and proliferate on all scaffolds; in turn, studies with skin cell models reveal that PCL:CA and PCL:CAP blends outperform all other substrates. Lung cell models show that, while 16HBE cells adhere to and proliferate in PCL, PCL:CA, PCL:EC, and PCL:MC scaffolds, A549 cells only have the same biological response on PCL, PCL:CA, and PCL:MC. In summary, all fibrous meshes prepared are biocompatible toward most cell types tested, thus suggesting the potential of PCL‐cellulose derivative blends as substrates suitable for in vitro epithelial tissue modeling and toxicity screening.

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Collagen-functionalized electrospun smooth and porous polymeric scaffolds for the development of human skin-equivalent

Abstract: Collagen-functionalized electrospun smooth and porous poly(l-lactide) scaffolds supporting keratinocytes and fibroblasts as a potential model to serve as self-organized skin substitute.

Cited by 21 publications

References 38 publications

Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds

Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds

Poly-l-Lactic Acid (PLLA)-Based Biomaterials for Regenerative Medicine: A Review on Processing and Applications

Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling

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