Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization

Abstract

Abstract:In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively.

Cosmetic Potential of Marine Fish Skin Collagen

“…Other sources such as marine collagen are arising as a relevant alternative to their mammalian counterparts. Several organisms such as marine sponges [5,6], jellyfish [7,8], squid [9,10], and fishes [11][12][13][14] have been exploited for the extraction of marine collagen. About 75% of a fish's…”

Collagen peptides from soft‑shelled turtle induce calpain‑1 expression and regulate inflammatory cytokine expression in HaCaT human skin keratinocytes

“…certain studies have demonstrated that collagen regulates cell proliferation or apoptosis (8,9). In this decade, collagens of marine origin (e.g., fish, sponges and mollusks) have been considered a useful resource due to their high availability (10)(11)(12)(13)(14)(15)(16)(17). These collagens have been widely used as functional foods or dietary supplements.…”

Shotgun label‐free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft‐shelled turtle

“…In addition, many Muslims and Jews do not consume pig‐derived food products, and many Hindus do not consume cow‐derived products . Therefore, collagen of marine origin, such as fish, sponges, and mollusks, was recently considered as a useful alternative to mammalian sources because of its high availability . In addition, we previously reported that soft‐shelled turtle ( Pelodiscus sinensis ) tissue could be a useful alternative for collagen .…”

“…17 Therefore, collagen of marine origin, such as fish, sponges, and mollusks, was recently considered as a useful alternative to mammalian sources because of its high availability. [18][19][20][21][22][23][24][25] In addition, we previously reported that soft-shelled turtle (Pelodiscus sinensis) tissue could be a useful alternative for collagen. 26 Recently, several reports demonstrated its usefulness, 27,28 making collagen from soft-shelled turtle a useful material for cosmetics, pharmaceuticals, and medical care products.…”