Collagen Film with Bionic Layered Structure and High Light Transmittance for Personalized Corneal Repair Fabricated by Controlled Solvent Evaporation Technique
Abstract:Corneal blindness is a common phenomenon, and corneal transplantation is an effective treatment for corneal defects. However, there is usually a mismatch between the corneal repair material and the degree of the patient’s corneal defect. Therefore, patients with different corneal defects need suitable corneal repair materials with a specific microstructure for personalized treatment. In this research, collagen films with bionic structures were fabricated through ethanol evaporation technique by regulating the … Show more
“…The water absorption performance of the Col-Tau film is 79.3 ± 3.5%. The results are very similar to the native cornea tissue (78.0 ± 3.0%), but significantly higher than the blank Col sample (67.9 ± 2.8%) [ 12 , 31 ]. The results indicate that the saturated water absorption of the Col-Tau film is promoted by the chemical grafting of the taurine’s sulfonic groups.…”
Section: Resultssupporting
confidence: 63%
“…The light transmittance of the pre-wetted Col-Tau and Col sample indicate minor differences. With the increase of wavelength, the light transmittance curve of the collagen-based films reached the highest level (higher than 90%), which is very similar to that of natural cornea tissue (about 87%) [ 11 , 12 , 31 ]. In addition, Figure 5 B exhibits a picture of pre-wetted Col-Tau sample.…”
Section: Resultsmentioning
confidence: 99%
“…To mitigate this problem, many researchers have tried to prepare various artificial materials using different macromolecules to reconstruct cornea scaffolds that mimic normal corneal tissue [ 7 , 8 , 9 , 10 ]. Collagen fibrils, as the predominant component of the human cornea, with good biocompatibility, biodegradability and biological activity, have been extensively studied for corneal repair and regeneration [ 11 , 12 , 13 ].…”
Corneal defects can seriously affect human vision, and keratoplasty is the most widely accepted therapy method for visual rehabilitation. Currently, effective treatment for clinical patients has been restricted due to a serious shortage of donated cornea tissue and high-quality artificial repair materials. As the predominant component of cornea tissue, collagen-based materials have promising applications for corneal repair. However, the corneal nerve repair and epithelization process after corneal transplantation must be improved. This research proposes a new collagen-based scaffold with good biocompatibility and biological functionality enhanced by surface chemical grafting of natural taurine molecular. The chemical composition of collagen-taurine (Col-Tau) material is evaluated by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, and its hydrophilic properties, light transmittance, swelling performance and mechanical tensile properties have been measured. The research results indicate that the Col-Tau sample has high transmittance and good mechanical properties, and exhibits excellent capacity to promote corneal nerve cell growth and the epithelization process of corneal epithelial cells. This novel Col-Tau material, which can be easily prepared at a low cost, should have significant application potential for the treating corneal disease in the future.
“…The water absorption performance of the Col-Tau film is 79.3 ± 3.5%. The results are very similar to the native cornea tissue (78.0 ± 3.0%), but significantly higher than the blank Col sample (67.9 ± 2.8%) [ 12 , 31 ]. The results indicate that the saturated water absorption of the Col-Tau film is promoted by the chemical grafting of the taurine’s sulfonic groups.…”
Section: Resultssupporting
confidence: 63%
“…The light transmittance of the pre-wetted Col-Tau and Col sample indicate minor differences. With the increase of wavelength, the light transmittance curve of the collagen-based films reached the highest level (higher than 90%), which is very similar to that of natural cornea tissue (about 87%) [ 11 , 12 , 31 ]. In addition, Figure 5 B exhibits a picture of pre-wetted Col-Tau sample.…”
Section: Resultsmentioning
confidence: 99%
“…To mitigate this problem, many researchers have tried to prepare various artificial materials using different macromolecules to reconstruct cornea scaffolds that mimic normal corneal tissue [ 7 , 8 , 9 , 10 ]. Collagen fibrils, as the predominant component of the human cornea, with good biocompatibility, biodegradability and biological activity, have been extensively studied for corneal repair and regeneration [ 11 , 12 , 13 ].…”
Corneal defects can seriously affect human vision, and keratoplasty is the most widely accepted therapy method for visual rehabilitation. Currently, effective treatment for clinical patients has been restricted due to a serious shortage of donated cornea tissue and high-quality artificial repair materials. As the predominant component of cornea tissue, collagen-based materials have promising applications for corneal repair. However, the corneal nerve repair and epithelization process after corneal transplantation must be improved. This research proposes a new collagen-based scaffold with good biocompatibility and biological functionality enhanced by surface chemical grafting of natural taurine molecular. The chemical composition of collagen-taurine (Col-Tau) material is evaluated by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, and its hydrophilic properties, light transmittance, swelling performance and mechanical tensile properties have been measured. The research results indicate that the Col-Tau sample has high transmittance and good mechanical properties, and exhibits excellent capacity to promote corneal nerve cell growth and the epithelization process of corneal epithelial cells. This novel Col-Tau material, which can be easily prepared at a low cost, should have significant application potential for the treating corneal disease in the future.
“…Collagen makes up about 70% of the dry-wet of the cornea and plays an important role in supporting CECs, LESCs and corneal fibroblast cell growth [4,6,9,10,23]. Three forms of collagen biomatrix are normally used in tissue engineering research, such as collagen sponge [45][46][47][48], collagen hydrogel [49][50][51] and collagen film [52][53][54].…”
Since the past few decades, numerous modifications and innovations have been done to design the optimal corneal biomatrix for corneal epithelial cells (CECs) or limbal epithelial stem cells (LESCs) carriers. However, researchers have yet to discover the ideal optimization strategies in the development of corneal biomatrix design and its effects on cultured CECs or LESCs. This review further discusses and summarizes recent optimization strategies to develop an ideal collagen biomatrix and its interaction with CECs and LESCs. Using PRISMA guidelines, the articles published from June 2012 to June 2022 were systematically searched using Web of Science (WoS), Scopus, PubMed, Wiley, and EBSCOhost databases. The literature search identified 444 potential relevant published articles, with 29 relevant articles selected based on the inclusion and exclusion criteria after the screening and appraising processes. The current paper highlights the physicochemical and biocompatibility (in vitro and in vivo) characterization methods, which were inconsistent throughout the different studies. Despite the variability in the methodology approach, the reviewer postulated that the modification of the collagen biomatrix improves its mechanical and biocompatibility properties toward CECs and LESCs. All findings were discussed in this review; thus, it provides a general view of up-to-date trends in this field.
“…Collagen makes up about 70% of the dry–wet weight of the cornea and plays an important role in supporting CECs, LESCs, and corneal fibroblast cell growth [ 4 , 6 , 9 , 10 , 23 ]. Three forms of collagen biomatrix are normally used in tissue engineering research, such as collagen sponge [ 45 , 46 , 47 , 48 ], collagen hydrogel [ 49 , 50 , 51 ], and collagen film [ 52 , 53 , 54 ].…”
Over the last several decades, numerous modifications and advancements have been made to design the optimal corneal biomatrix for corneal epithelial cell (CECs) or limbal epithelial stem cell (LESC) carriers. However, researchers have yet to discover the ideal optimization strategies for corneal biomatrix design and its effects on cultured CECs or LESCs. This review discusses and summarizes recent optimization strategies for developing an ideal collagen biomatrix and its interactions with CECs and LESCs. Using PRISMA guidelines, articles published from June 2012 to June 2022 were systematically searched using Web of Science (WoS), Scopus, PubMed, Wiley, and EBSCOhost databases. The literature search identified 444 potential relevant published articles, with 29 relevant articles selected based on inclusion and exclusion criteria following screening and appraising processes. Physicochemical and biocompatibility (in vitro and in vivo) characterization methods are highlighted, which are inconsistent throughout various studies. Despite the variability in the methodology approach, it is postulated that the modification of the collagen biomatrix improves its mechanical and biocompatibility properties toward CECs and LESCs. All findings are discussed in this review, which provides a general view of recent trends in this field.
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