Collagen fibrils as skeletal frameworks in the monkey trabecular meshwork

Spatial mapping of collagen fibril organisation in primate cornea—an X-ray diffraction investigation

“…It is likely that a significant proportion of the alignment we observe in the marmoset annulus is located in more posterior parts of the cornea and in particular the trabecular meshwork. Indeed, Nishida and Mizutani (1994) observed the primate trabecular meshwork using electron microscopy and concluded that in peripheral regions of the corneo-scleral sheet most collagen bundles appear to run parallel to the circumference of the limbus.…”

“…As early as 1966, it was noted that the fibrillary texture of monkey cornea closely resembled that of man (Ehlers, 1966). However, subsequent investigation of stromal collagen organisation to date has been limited to highly localised studies of the central cornea (Jacobsen et al, 1984;Ozanics et al, 1976Ozanics et al, , 1977 and the trabecular meshwork (Nishida and Mizutani, 1994). Collectively, the literature points to a lamellar arrangement and general morphology grossly similar to that seen in human cornea, while small-angle X-ray scattering (SAXS) patterns from the centre of macaque monkey corneas have disclosed collagen fibrils only marginally larger and more widely spaced than those observed in humans (Meek and Leonard, 1993).…”

Three-Dimensional Arrangement of Collagen Fibrils in Human Ciliary Body

“…With the NaOH cellmaceration method, which removes cellular and extracellular elements while preserving the collagen fibrils [8,[15][16][17][18][19], we have demonstrated the three-dimensional architecture of the collagen fibrils in the ciliary body unobscured by other tissues or cells in the present study. We visualized the orientation of the collagen fibrils or fiber bundles in various parts of the ciliary body and the special structure of the 'honeycomb' in Bruch's mem- The general morphology appears to be similar to that in vivo.…”

“…Recently, Ohtani et al [8] have developed a cell-maceration method with NaOH solution for SEM observation, which permitted the observation of the three-dimensional architecture of the collagen fibrils in tissues. Studies with this method have been successful in various tissues or organs and have brought us much new information about the collagen [8,[15][16][17][18][19]. The purposes of the present study, therefore, were to visualize the three-dimensional architecture of the collagen fibrils in the aged ciliary body with this new method and discuss its significance.…”