Collagen fibers in the rectus abdominis muscle of cadavers of different age

Calvi, E. N. C.; Nahas, Fábio Xerfan; Barbosa, Marcus Vinícius Jardini; Calil, José Augusto; Ihara, Sílvia Saiuli Miki; Juliano, Yára; Ferreira, Lydia Masako

doi:10.1007/s10029-014-1213-0

Cited by 17 publications

(9 citation statements)

References 27 publications

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“…The study in male cadavers was divided into two broad age groups (18-30 years and 31-60 years), with similar results in collagen composition in linea alba in the supra and infra umbilical regions, compared with the results of the groups in this study. The decrease in the amount of collagen type I and III was observed in the older age group, reinforcing the theory of weakness in the abdominal wall over the years, even in patients that were not distributed according to risk factors 4 . Even in studies that point to a previously existent incisional hernia, age is classified as a risk factor for adverse events in the postoperative period 3 , 16 .…”

Section: Discussionsupporting

confidence: 71%

Linea Alba Collagen Assessment in Morbidly Obese Patients

Grossi

Nicola

Zepeda

et al. 2016

ABCD, arq. bras. cir. dig.

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Background:The evaluation of collagen in the abdominal wall has been increasingly studied because of the relevance on collagen in the healing process after laparotomy. Aim: To evaluate the amount of collagen in the linea alba of patients undergoing laparotomic bariatric surgery and comparing with non-obese cadavers. Methods:Were evaluated 88 samples of aponeurosis from abdominal linea alba of 44 obese patients (obesity group) and 44 non-obese cadavers (control group). The samples were collected in 2013 and 2104, and were sorted according to age (18-30, 31-45 and 46-60), gender, BMI, waist and cervical circumference, and subcutaneous tissue thickness. Material for biopsy was collected from the supraumbilical region of the linea alba for immunohistochemical analysis differentiating collagen type 1 and type 3 and the 1/3 ratio. Image-Pro Plus pixel counting software was used to measure the amount of collagen. Results:The obesity group evidenced mean age 44.11±9.90 years; 18-30 age group had three (6.8%) obese individuals; 31-45 had 22 (50%) and 46-60 had 19 (43.1%). Females were present in 81.8% (n=36); BMI (kg/m²) was 48.81±6.5; waist circumference (cm) was 136.761±13.55; subcutaneous tissue thickness (cm) 4.873±0.916. Considering age groups, gender and BMI, there were statistical differences in all tests when compared with the cadavers. Conclusion:The amount of collagen in the linea alba above the umbilical region in the morbidly obese patients was smaller than in the non-obese cadavers in the same age group.

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Section: Discussionsupporting

confidence: 71%

Linea Alba Collagen Assessment in Morbidly Obese Patients

Grossi

Nicola

Zepeda

et al. 2016

ABCD, arq. bras. cir. dig.

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“…Two pitfalls of the histology analysis performed in this study were that Masson’s trichrome stain was not used to evaluate collagen fibre arrangement 27 and that immunohistochemical techniques were not used to detect vascularity by identifying CD31 positive cells in the tendon histology sections 28 . However, the tendons were evaluated by using a classic tendon histology score 29 30 , in which the tissue samples were stained with haematoxylin and eosin and alcian blue.…”

Section: Discussionmentioning

confidence: 99%

Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model

Gonzalez

López

Álvarez

et al. 2016

Sci Rep

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Leukocyte-reduced platelet-rich plasma (LR-PRP) is a therapy for tendinopathy of the Achilles tendon (TAT); however, there is scarce information regarding LR-PRP effects in rabbit models of TAT. We compared, at 4 and 12 weeks (w), the LR-PRP and placebo (PBS) effects on ultrasonography, histology and relative gene expression of collagen types I (COL1A1) and III (COL3A1) and vascular endothelial growth factor (VEGF) in 24 rabbits with TAT induced by collagenase. The rabbits (treated with both treatments) were euthanatised after either 4 or 12 w. A healthy group (HG (n = 6)) was included. At 4 and 12 w, the LR-PRP group had a no statistically different histology score to the HG. At w 4, the COL1A1 expression was significantly higher in the LR-PRP group when compared to HG, and the expression of COL3A1from both LR-PRP and PBS-treated tendons was significantly higher when compared to the HG. At w 12, the expression of COL3A1 remained significantly higher in the PBS group in comparison to the LR-PRP group and the HG. At w 4, the LR-PRP group presented a significantly higher expression of VEGF when compared to the PBS group and the HG. In conclusion, LR-PRP treatment showed regenerative properties in rabbits with TAT.

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“…Type I collagen was negatively correlated with passive excursion, while type III was positively correlated with passive excursion. Type I and III collagen are important components of the perimysium and epimysium, and their relative amounts differ between species and between muscle groups within the same species [10, 11]. Therefore, different ratios might be observed between type I and type III collagen content if excursion is analyzed according to hydroxyproline content.…”

Section: Discussionmentioning

confidence: 99%

“…We reported that muscle excursion was decreased and collagen content was increased over time after tenotomy and that collagen content was negatively correlated with passive excursion [9]. Type I, III, and IV collagen are the main subtypes of muscle collagen [10, 11]. It is thought that type I collagen resists tension and type III collagen provides structural stability to expandable organs [12].…”

Section: Introductionmentioning

confidence: 99%

Correlation between change in muscle excursion and collagen content after tendon rupture and delayed repair

Koh

Kang

et al. 2017

J Orthop Surg Res

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BackgroundThe objectives of the present study were to compare changes in muscle excursion, total collagen, and collagen subtypes after tenotomy over time and after delayed tendon repair.MethodsTenotomy on the extensor digitorum tendon of the right second toes of 48 New Zealand White rabbits was performed; toes on the left leg were used as controls. Passive muscle excursion, total collagen content, and type I, III, and IV collagen contents were measured at 1, 2, 4, and 6 weeks after tenotomy. Next, passive muscle excursion and total collagen content were measured at 8 weeks after delayed tendon repair at 1, 2, 4, and 6 weeks after a tenotomy.ResultsPassive muscle excursion decreased sequentially over time after tenotomy. Meanwhile, total collagen increased over time. These changes were significant after 4 weeks of injury. Type I collagen significantly increased, type III collagen significantly decreased, and type IV collagen had no significant change over time. Passive muscle excursion was negatively correlated with total collagen and type I collagen after tenotomy at each time point after tenotomy (p < 0.05). After tendon repair, increases in total collagen content after tenotomy were not reversed, despite early repairs at 1 and 2 weeks after tenotomy.ConclusionsIncreases in type I collagen were found to be associated with decreased excursion after tendon rupture. The increase in collagen that was observed after tenotomy was not reversed by repair within 8 weeks.

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Collagen fibers in the rectus abdominis muscle of cadavers of different age

Abstract: The amounts of types I and III collagen in the rectus abdominis muscle were significantly smaller in older subjects.

Cited by 17 publications

References 27 publications

Linea Alba Collagen Assessment in Morbidly Obese Patients

Linea Alba Collagen Assessment in Morbidly Obese Patients

Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model

Correlation between change in muscle excursion and collagen content after tendon rupture and delayed repair

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